Cyclodextrin‐Based Bimodal Fluorescence/MRI Contrast Agents: An Efficient Approach to Cellular Imaging

Kotková, Zuzana; Kotek, Jan; Jirák, Daniel; Jendelová, Pavla; Herynek, Vı́t; Berková, Zuzana; Hermann, Petr; Lukeš, Ivan

doi:10.1002/chem.200903519

Cited by 50 publications

(36 citation statements)

References 77 publications

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“…Conjugates 295d are biocompatible and no toxicity was detected. The contrast agents prepared were used for imaging pancreatic islets or stem cells [224]. The MRI probe based on a cyclodextrin scaffold was internalized into cells and resulted in sufficient labelling for MRI visualization.…”

Section: Phosphinic Acids For Mri or Radioligand Deliverymentioning

confidence: 99%

Synthesis and Biological Applications of Phosphinates and Derivatives

Virieux

Volle

Bakalara

et al. 2014

Topics in Current Chemistry

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This review first outlines general considerations on phosphinic acids and derivatives as bioisosteric groups. The next sections present key aspects of phosphinic acid-based molecules and include a brief description of the biological pathways involved for their activities. The synthetic aspects and the biological activities of such compounds reported in the literature between 2008 and 2013 are also described.

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Section: Phosphinic Acids For Mri or Radioligand Deliverymentioning

confidence: 99%

Synthesis and Biological Applications of Phosphinates and Derivatives

Virieux

Volle

Bakalara

et al. 2014

Topics in Current Chemistry

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“…The use of contrast agents for MRI provides comparable spatial resolution with far better contrast resolution than CT imaging (21,22). Furthermore, the use of both MRI and fluorescent labeling (i.e., bimodal imaging) to track proteins, cells, and angiogenesis was recently reported (23)(24)(25).…”

Section: Significancementioning

confidence: 99%

“…Bimodal MRI/fluorescence imaging stands to become a powerful research tool in the pursuit of optimized biodegradable materials for tissue regeneration. Even though various methods have been developed to document the fate of biomaterials and to track cellular events in vivo (24,(49)(50)(51), none of these has been able to noninvasively monitor the precise dynamics of implant integration. The rate at which a biomaterial is degraded has usually been quantified using in vitro methods (14,20,52,53), which are poorly correlated with in vivo resorption of the material and offer limited insight into the ultimate fate of the implant.…”

Section: Significancementioning

confidence: 99%

Using bimodal MRI/fluorescence imaging to identify host angiogenic response to implants

Berdichevski

Yameen

Dafni³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Therapies that promote angiogenesis have been successfully applied using various combinations of proangiogenic factors together with a biodegradable delivery vehicle. In this study we used bimodal noninvasive monitoring to show that the host response to a proangiogenic biomaterial can be drastically affected by the mode of implantation and the surface area-to-volume ratio of the implant material. Fluorescence/MRI probes were covalently conjugated to VEGF-bearing biodegradable PEG-fibrinogen hydrogel implants and used to document the in vivo degradation and liberation of bioactive constituents in an s.c. rat implantation model. The hydrogel biodegradation and angiogenic host response with three types of VEGFbearing implant configurations were compared: preformed cylindrical plugs, preformed injectable microbeads, and hydrogel precursor, injected and polymerized in situ. Although all three were made with identical amounts of precursor constituents, the MRI data revealed that in situ polymerized hydrogels were fully degraded within 2 wk; microbead degradation was more moderate, and plugs degraded significantly more slowly than the other configurations. The presence of hydrogel degradation products containing the fluorescent label in the surrounding tissues revealed a distinct biphasic release profile for each type of implant configuration. The purported in vivo VEGF release profile from the microbeads resulted in highly vascularized s.c. tissue containing up to 16-fold more capillaries in comparison with controls. These findings demonstrate that the configuration of an implant can play an important role not only in the degradation and resorption properties of the materials, but also in consequent host angiogenic response.biomaterial scaffold | angiogenesis | hydrogel | contrast agents | tissue regeneration W ith progress in the field of regenerative medicine relying more on approaches that deliver cells and/or bioactive factors using minimally invasive procedures, functional donor-tohost integration remains a critical challenge. In this context, biodegradable hydrogel scaffolds provide certain advantages, such as excellent tissue compatibility, temporary protection from host inflammation, and controlled resorption based on cell-mediated degradation (1). Effective transport properties to and within an implanted hydrogel are critical in the design of cell-seeded scaffolds, where the delivery of nutrients and removal of waste products from regions deep within the implant can severely limit the use of a patch for only the smallest of grafts (i.e., <0.5 mm) (2-4). Therefore, a great deal of research in the field of regenerative medicine has been devoted to ameliorate the survival of the cells and tissues with implants that use proangiogenic factors.One strategy is to enhance the localized formation of vascularized networks to improve the perfusion of oxygen and nutrients to the intended region in the body (2, 4, 5). This process is regulated by a number of different growth factors that stimulate a complex angiogenic res...

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“…The aromatic amino group has been converted into isothiocyanate that can be coupled with other amines. This reaction sequence was used for synthesis of a ditopic ligand [316] structures with dendrimeric or cyclodextrin cores [317,318]. Aqueous conditions has also been used for preparation of monophosphinate bearing 2-carboxyethyl group [319].…”

Section: Derivatives Containing Phosphorus Acid Pendant Arm(s)mentioning

confidence: 99%