Cyclodextrin‐modified capillary electrophoresis for achiral and chiral separation of ergostane and lanostane compounds extracted from the fruiting body of Antrodia camphorata

Abstract

A CD-modified capillary electrophoretic method has been developed for achiral and chiral analysis of seven bioactive compounds isolated from the fruiting body of Antrodia camphorata. Such important target analytes exhibit similar chemical structures and are known for their diverse properties including antioxidant and anticancer effects. The analytes were separated in 25 min using a pH 9.3, 20 mM sodium borate buffer containing 20 mM methyl-b-CD and 30 mM sulfobutylether-b-CD. With the exception of the optical isomer pairs (antcin B or zhankuic acid A, zhankuic acid C, and antcin A), the remaining bioactive compounds including the chiral pair antcin C were baseline-separated. Analysis time was noticeably longer to baseline separate all of the above chiral pairs ($38 min) by adding 5% DMF to the running buffer. The migration order was reversed compared with the HPLC elution. More hydrophobic compounds complexed favorably with methyl-b-CD and emerged earlier in the electropherogram than their more hydrophilic counterparts which were strongly associated with sulfobutylether-b-CD. The simple capillary electrophoretic method developed was applicable for rapid separation and characterization of several important bioactive compounds isolated from the fruiting body of A. camphorata.

Review of Pharmacological Effects of Antrodia camphorata and Its Bioactive Compounds

“…Thus, the combined, synergistic effects of a mixture of active components that are present in A. camphorata on biological activities need to be thoroughly assessed. Finally, though we recently developed a cyclodextrin-modified capillary electrophoresis method for the separation and analysis of achiral and chiral triterpenoids from fruiting bodies of A. camphorata [110], there however, is a need to establish suitable quality parameters and analytical methods to determine active compounds.…”

Macrophage Mediated Anti-Proliferation Effects ofAnthodia camphorataNon-Polysaccharide Based Extracts on Human Hepatoma Cells

“…Taking the data from the HPLC and GPC analyses together, we categorized AC-PS as one group with abundant polysaccharides and AC-FB (or AC-SS) as another group with abundant triterpenoids and unidentified constituents. 31,32) In view of the different constituents of AC-PS and AC-FB (and AC-SS) a discrepancy in their antiproliferation effects plausible. This further suggests that AC-FB and AC-SS elicit different immunomodulatory responses from the macrophages than AC-PS.…”

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

“…A large number of CE‐UV analysis have been performed in pharmaceutical and herbal product applications 165–195. Most of these were applied for the separation of bioactive diterpenoids, triterpenes, flavonoids, and phenolic compounds.…”