Cyclophilin A Is Required for Retinoic Acid-induced Neuronal Differentiation in p19 Cells

Song, Jun; Lu, Yingchun; Yokoyama, Kazunzri K.; Rossi, John J.; Chiu, Robert

doi:10.1074/jbc.m311406200

Cited by 35 publications

(26 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 B, panels f and i; and E). Thus, these results are consistent with a previous report except for the suppression of gliogenesis (45), suggesting that CypA participates in the early stages of neural differentiation.…”

Section: Resultssupporting

confidence: 83%

“…12 and 14). It has also been reported that, in P19 cells, knockdown of CypA by RNA interference causes loss of RA-primed neuronal differentiation, but not dimethyl sulfoxide-induced mesodermal differentiation, and a marked reduction of RA-induced RARE-mediated reporter activity, showing that CypA promotes RARE-mediated neurogenic gene expression (45). Thus, the enhancement of astrocytogenesis by the forced expression of Prp19␤ RNA might be due at least in part to the inhibition of neurogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Sequence analysis of the clones showed that three of the 10 clones were novel cDNA clones, and six were identified as known cDNA clones, such as CypA; prothymosin-␣; endometrial bleeding-associated factor; proteasome activator subunit-4; and ribosomal proteins S7, S15a, and S26. Among them, CypA is specifically expressed in neurons and participates in the neuronal (but not astroglial) differentiation of P19 cells (44,45). Therefore, in this study, we focused on the functional analysis of CypA in neural differentiation regulated by PRP19␣ and PRP19␤.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Involvement of the Mouse Prp19 Gene in Neuronal/Astroglial Cell Fate Decisions

Urano

Iiduka

Sugiyama

et al. 2006

Journal of Biological Chemistry

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Involvement of the Mouse Prp19 Gene in Neuronal/Astroglial Cell Fate Decisions

Urano

Iiduka

Sugiyama

et al. 2006

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…Furthermore, CypA is involved in multiple signaling events, for example, it regulates IL-2 tyrosine kinase activity (Brazin et al, 2002), is required for retinoic acid-induced neuronal differentiation (Song et al, 2004) and modulates prolactin receptor signaling (Syed et al, 2003). CypA also affects gene expression by physically interacting with transcription factors and histone-modifying enzymes (Arevalo-Rodriguez et al, 2000;Cui et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Cyclophilins contribute to Stat3 signaling and survival of multiple myeloma cells

et al. 2009

View full text Add to dashboard Cite

Signal transducer and activator of transcription 3 (Stat3) is the major mediator of interleukin-6 (IL-6) family cytokines. In addition, Stat3 is known to be involved in the pathophysiology of many malignancies. Here, we show that the cis-trans peptidyl-prolyl isomerase cyclophilin (Cyp) B specifically interacts with Stat3, whereas the highly related CypA does not. CypB knockdown inhibited the IL-6-induced transactivation potential but not the tyrosine phosphorylation of Stat3. Binding of CypB to Stat3 target promoters and alteration of the intranuclear localization of Stat3 on CypB depletion suggested a nuclear function of Stat3/CypB interaction. By contrast, CypA knockdown inhibited Stat3 IL-6-induced tyrosine phosphorylation and nuclear translocation. The Cyp inhibitor cyclosporine A (CsA) caused similar effects. However, Stat1 activation in response to IL-6 or interferon-c was not affected by Cyp silencing or CsA treatment. As a result, Cyp knockdown shifted IL-6 signaling to a Stat1-dominated pathway. Furthermore, Cyp depletion or treatment with CsA induced apoptosis in IL-6-dependent multiple myeloma cells, whereas an IL-6-independent line was not affected. Thus, Cyps support the anti-apoptotic action of Stat3. Taken together, CypA and CypB both play pivotal roles, yet at different signaling levels, for Stat3 activation and function. These data also suggest a novel mechanism of CsA action.

show abstract

“…We then examined the biological effects of the knockdown of CypA. We previously found that CypA is required for retinoic acid-induced neuronal differentiation of p19 cells (4). We examined the expression β-tubulin III, a marker for neuronal differentiation in different knockdown cells with or without retinoic acid treatment.…”

Section: Gene Silencing By Multiple Rnai Targetingmentioning

confidence: 99%