Cyfluthrin and Deltamethrin Induce Changes in the Fat Body Composition of <i>Tenebrio molitor</i> Larvae, Males and Females

Wojciechowska, Marta; Stepnowski, Piotr; Gołębiowski, Marek

doi:10.1002/cbdv.201800515

Cited by 10 publications

(9 citation statements)

References 38 publications

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“…However, such a strategy of application would not mimic the mode of agrochemical treatments we wanted to follow. This is confirmed by the work of Wojciechowska et al [ 35 ], where the composition of the fat body changed in terms of the quality of compounds such as amino acids, fatty acids, cholesterol and carboxylic acids, in larvae treated with various types of insecticides. However, in our studies, particular fractions of lipids were not analysed.…”

Section: Resultssupporting

confidence: 65%

Solanum nigrum Fruit Extract Increases Toxicity of Fenitrothion—A Synthetic Insecticide, in the Mealworm Beetle Tenebrio molitor Larvae

Spochacz

Szymczak

Chowański

et al. 2020

Toxins

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Synthetic insecticides are widely used for crop protection both in the fields and in the food stored facilities. Due to their toxicity, and assumptions of Integrated Pest Management, we conducted two independent experiments, where we studied the influence of Solanum nigrum unripe fruit extract on the toxicity of an organophosphorus insecticide fenitrothion. In the first variant of the experiment, Tenebrio molitor larvae were fed with blended fenitrothion (LC50) and the extract in four concentrations (0.01, 0.1, 1 and 10%) in ratio 1:1 for 3 days. In the second variant, a two-day application of fenitrothion (LC40) was preceded by a one-day extract treatment. The first variant did not show any increase in lethality compared to fenitrothion; however, ultrastructure observations exhibited swollen endoplasmic reticulum (ER) membranes in the midgut and nuclear and cellular membranes in the fat body, after application of blended fenitrothion and extract. An increased amount of heterochromatin in the fat body was observed, too. In the second variant, pre-treatment of the extract increased the lethality of larvae, decreased the level of glycogen and lipids in the fat body and disrupted integrity of midgut cellular membranes. S. nigrum extract, applied prior to fenitrothion treatment can be a factor increasing fenitrothion toxicity in T. molitor larvae. Thus, this strategy may lead to decreased emission of synthetic insecticides to the environment.

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Section: Resultssupporting

confidence: 65%

Solanum nigrum Fruit Extract Increases Toxicity of Fenitrothion—A Synthetic Insecticide, in the Mealworm Beetle Tenebrio molitor Larvae

Spochacz

Szymczak

Chowański

et al. 2020

Toxins

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“…The second insecticide used was K‐othrine (1.0 %), which contains deltamethrin C 22 H 19 Br 2 NO 3 as the active substance. Cyfluthrin and deltamethrin are soluble in organic solvents . These are synthetic compounds classified as pyrethroids.…”

Section: Methodsmentioning

confidence: 99%

“…For the final determinations, a gas chromatography technique coupled with mass spectrometry (GC/MS) was used, which is characterized by high sensitivity and selectivity. The analyses were performed with a Shimadzu QP 2010s gas chromatograph using a ZB5 capillary column (Zebron 30 m×0.25 mm×0.25 mm) . Analysis conditions: carrier gas, helium; analysis time, 55 min; desorption time, 5 min; analysis temperature, 250 °C; column temperature, 40 °C; temperature of analysis, 40 °C to 250 °C with an increase of 6 °C/min; ion source temperature, 200 °C; interface temperature, 250 °C …”

Section: Methodsmentioning

confidence: 99%

SPME‐GC/MS Analysis of Volatile Compounds Contained in the Insect Larvae of Tenebrio molitor and Leptinotarsa decemlineata before and after Using Insecticides

Wojciechowska

Gołębiowski

2020

Chemistry & Biodiversity

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The larvae of two harmful insect species Tenebrio molitor and Leptinotarsa decemlineata were analyzed. The insects were sprayed with insecticides containing the active substances cyfluthrin and deltamethrin (T. molitor), and thiamethoxam and acetamiprid (L. decemlineata). The sprayed insect larvae were left for 24, 48 and 72 h. Samples were then prepared using SPME fiber to identify the volatile compounds contained in the larvae. The determinations were made by gas chromatography coupled with mass spectrometry (GC/MS). Chemical compounds were found in the analyzed samples: alkanes, aldehydes, ketones, esters, terpenes, fatty acids. In the samples prepared from T. molitor larvae, more than 20 % of aldehydes and 8 -41 % of alkanes were determined. In the samples from L. decemlineata, fatty acids were 8-65 % depending on the length of time after applying the insecticide.

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“…This might suggest that the presence of these sterols in pupae is connected with their role as substrates for cholesterol synthesis; however, again, more research is needed to confirm this thesis. Stigmastanol was also observed in the internal extract from T. molitor, after treatment with cyfluthrin-containing insecticide [108].…”

Section: Discussionmentioning

confidence: 91%

The Impact of the Entomopathogenic Fungus Conidiobolus coronatus on the Free Fatty Acid Profile of the Flesh Fly Sarcophaga argyrostoma

Kaczmarek

Boguś

2021

Insects

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The chemical composition of the insect cuticle varies remarkably between species and their life stages. It can affect host resistance and substrate utilization by invading entomopathogen fungi, such as the soil fungus Conidiobolus coronatus. In this study, Sarcophaga argyrostoma flies were exposed to sporulating C. coronatus colonies for 24 h; the pupae were resistant, but the adults demonstrated 60% mortality. Although the pupae demonstrated no sign of infection nor any abnormal development, our findings indicate that after 24 h of contact with the fungus, the pupae demonstrated a 25.2-fold increase in total cuticular free fatty acids (FFAs) and a 1.9-fold decrease in total internal FFAs. Also, the cuticular FFA increased from 26 to 30, while the internal FFA class increased from 13 to 23. In exposed adults, the total mass of cuticular FFAs increased 1.7-fold, while the number of FFAs stayed the same (32 FFAs). Also, the internal FFA class increased from 26 to 35 and the total FFA mass increased 1.1-fold. These considerable differences between adults and pupae associated with C. coronatus exposure indicate developmental changes in the mechanisms governing lipid metabolism and spatial distribution in the organism, and suggest that cuticular lipids play a vital role in the defence against pathogenic fungi.

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Cyfluthrin and Deltamethrin Induce Changes in the Fat Body Composition of Tenebrio molitor Larvae, Males and Females

Cited by 10 publications

References 38 publications

Solanum nigrum Fruit Extract Increases Toxicity of Fenitrothion—A Synthetic Insecticide, in the Mealworm Beetle Tenebrio molitor Larvae

Solanum nigrum Fruit Extract Increases Toxicity of Fenitrothion—A Synthetic Insecticide, in the Mealworm Beetle Tenebrio molitor Larvae

SPME‐GC/MS Analysis of Volatile Compounds Contained in the Insect Larvae of Tenebrio molitor and Leptinotarsa decemlineata before and after Using Insecticides

The Impact of the Entomopathogenic Fungus Conidiobolus coronatus on the Free Fatty Acid Profile of the Flesh Fly Sarcophaga argyrostoma

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