Cysteine proteases from plants: Utilization in foods and nutraceuticals and characterization approaches for quality management

Benito-Vázquez, Iván; Garrido-Romero, Manuel; Díez-Municio, Marina; Moreno, F. Javier

doi:10.1016/j.afres.2024.100491

Applied Food Research

2024

DOI: 10.1016/j.afres.2024.100491

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Cysteine proteases from plants: Utilization in foods and nutraceuticals and characterization approaches for quality management

Iván Benito-Vázquez,

Manuel Garrido-Romero,

Marina Díez-Municio

et al.

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Cited by 1 publication

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New Pipeline for Analysing Fruit Proteolytic Products Used as Digestive Health Nutraceuticals

Benito-Vázquez,

Muñoz-Labrador,

Garrido-Romero

et al. 2024

IJMS

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Proteolytic products are extensively used in the nutraceutical sector to improve protein digestion and muscle quality in target populations (e.g., athletes or elderly). These products are processed using techniques that often lead to low purity but competitive pricing. Despite their widespread use and well-established production methods, the industry lacks standardized analytical methods for assessing these products and detecting potential fraud. This study proposes a comprehensive and harmonized pipeline for their analysis, which includes quantifying total soluble protein and proteolytic activity, as well as the determination of product stability and protein profile using SDS-PAGE and proteomic techniques. Despite the fact that protease extracts from pineapple had the highest protein content, most of the bromelain remained inactive, unlike in kiwi and papaya. SDS-PAGE revealed partial protein degradation of pineapple extracts, whereas kiwi extracts reflected a lower purification level but a higher protein integrity. The application of proteomic approaches strengthened the identification and origin tracing of the proteases. This study contributes to the development of a robust framework for analyzing proteolytic extracts, spanning from soluble protein quantification to protein profiling and activity determination. It may also ensure reliable supplier selection, high-quality manufacturing practices, and the implementation of optimal storage and formulation strategies in the nutraceutical industry.

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New Pipeline for Analysing Fruit Proteolytic Products Used as Digestive Health Nutraceuticals

Benito-Vázquez,

Muñoz-Labrador,

Garrido-Romero

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

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Cysteine proteases from plants: Utilization in foods and nutraceuticals and characterization approaches for quality management

Cited by 1 publication

References 160 publications

New Pipeline for Analysing Fruit Proteolytic Products Used as Digestive Health Nutraceuticals

New Pipeline for Analysing Fruit Proteolytic Products Used as Digestive Health Nutraceuticals

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