Cyto-toxicity, biocompatibility and cellular response of carbon dots–plasmonic based nano-hybrids for bioimaging

Abstract

In this study, hybrid carbon dots-plasmonic nanostructures including carbon dots/polyethyleneimine/gold (C-dots/PEI/Au), and carbon dots/polyethyleneimine/silver (C-dots/PEI/Ag) have been prepared using a microwave irradiation method. The prepared hybrid nanostructures have been characterized via optical spectroscopy, high resolution transmission electron microscopy (HRTEM), and X-ray diffraction (XRD).A remarkable enhancement in the optical parameters, such as absorptivity and quantum yield (QY), has been observed for the hybrid nanostructure compared to pure carbon dots. This plasmonic enhancement was more pronounced in the presence of silver (C-dots/PEI/Ag nanohybrid) than that of gold (C-dots/PEI/Au nanohybrid). This is referred to the low intrinsic loss and the degree of the overlap between the absorption spectra of silver nanoparticles and carbon dots. Furthermore, the biocompatibility assay and cellular response on epithelial kidney (Vero) normal cell has been investigated.The results showed that the optimal dose of treatment is about $200 mg ml À1 using both C-dots/PEI/Au or C-dots/PEI/Ag, nano-hybrids could be used safely in diagnostic bioimaging applications.

Carbon dots derived from algae as H2O2 sensors: the importance of nutrients in biomass

“…This PLE peak is attributed to the n-p* transition of -C]O, C-N or -C-OH bonds in the sp 3 hybridized domains, which originated from the carboxyl (-COOH), hydroxyl or amine (-NH 2 ) groups on the C-dot surface. 45 This PLE peak is in accordance with the parasitic peak at a similar position in the UV-vis light-absorption. It is known that the p-p* transition of the aromatic -C]Cunder sp 2 hybridization shows an absorption peak at 250 nm, 45,46 but no PLE peak was found in this region, indicating that sp 3 hybridized bonds are responsible for the uorescence.…”

Biological and Spectroscopic Investigations of New Tenoxicam and 1.10-Phenthroline Metal Complexes

“…The cytotoxicity activity against HCT-116, HepG2, and MCF-7 human cancer cell lines was estimated using the 3-[4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, which is based on the cleavage of the tetrazolium salt by mitochondrial dehydrogenases in viable cells [50][51][52]. Cells were dispensed in a 96 well sterile microplate (5 × 10 4 cells/well), and incubated at 37 • C with series of different concentrations (3.12, 6.25, 12.5 and 25.0 µM), in DMSO, of each tested compound or Doxorubicin (positive control) for 48 h in a serum free medium prior to the MTT assay.…”

<p>Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines</p>

“…Compusyn software (version 1.0, ComboSyn, Inc., Paramus, NJ, USA) was used to predict and simulate the combination index (CI) and fraction effect (fa) of the CuCs nanocomposite to estimate its activity and the amount of drug released into cells. 28 Simulated CuCs nanocomposite was used to investigate its cellular response using a response additivity model (Linear Interaction Effect). Such a model can demonstrate the positive effect of a drug combination (chitosan and curcumin) that might occur when the combination effect (E AB ) is greater than the expected additive effect given by the sum of the individual effects (E A +E B ).…”