Cytochalasin-B modulation of intestinal epithelial tight junction is mediated by myosin light chain kinase

Ma, Thomas Y.; Bui, Vuong Nghia; Mills, Susan; Pedram, Ali; Dat, Tran Do

doi:10.1016/s0016-5085(98)84185-1

Cited by 2 publications

(3 citation statements)

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“…22,25,26) The permeability of the Caco-2 cell monolayers to Di-4S and Di-6S was markedly increased when the TER value of the monolayers was reduced by Cyto B (Fig. 4) and also by IFN- (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Permeation of Disaccharides Derived from Chondroitin Sulfate through Human Intestinal Caco-2 Cell Monolayersviathe Paracellular Pathway

Jin

Satsu

Yamada

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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The intestinal absorption of chondroitin sulfate (CS) and its hydrolysate, particularly the disaccharides (Di-CS), was investigated by using human intestinal epithelial Caco-2 cell monolayers. Although the transepithelial transport of CS was not detectable, dose- and time-dependent transport of Di-CS was observed. The transport of Di-CS was proved to be energy-independent and its permeability increased inversely with the transepithelial electrical resistance (TER) value of the Caco-2 cell monolayers. The transport rate for Di-CS was also increased by treating the cell monolayers with such tight junction-opening agents as interferon-gamma. These results suggest that Di-CS permeated across the Caco-2 cell monolayers mainly via the paracellular pathway. The permeability of Caco-2 cell monolayers to authentic chondroitin 4-sulfate disaccharides (Di-4S), chondroitin 6-sulfate disaccharides (Di-6S) and chondroitin 0-sulfate disaccharides (Di-0S) was almost same, suggesting that sulfation did not affect the transport rate of Di-CS.

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Section: Discussionmentioning

confidence: 99%

“…25,26) When Caco-2 cell monolayers were treated with Cyto B (5 mg/ml) on the apical side, their TER value was decreased by almost 70% (Fig. 4A top).…”

Section: Energy-dependence Of the Transepithelial Transport Of Di-csmentioning

confidence: 99%

Permeation of Disaccharides Derived from Chondroitin Sulfate through Human Intestinal Caco-2 Cell Monolayersviathe Paracellular Pathway

Jin

Satsu

Yamada

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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“…Next, we used cytochalasin B as a tight junction disruptor to investigate the involvement of the paracellular pathway in the transepithelial permeation of Abi and Ahe. Cytochalasin B increases the paracellular permeability by affecting the tight junction structure through the myosin light chain kinase activation (14). Treatment with 5 µg/ml cytochalasin B significantly decreased the TEER value of the Caco-2 cell monolayers and increased the transepithelial permeability of Abi and Ahe (Figures 5C,D), indicating that Abi and Ahe mainly permeate through the Caco-2 cell monolayers via the paracellular pathway.…”

Section: Analysis Of the Transepithelial Permeation Route Of Abi And Ahementioning

confidence: 95%

Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats

Shirai

Karasawa²,

Kodaira³

et al. 2022

Front. Nutr.

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Agaro-oligosaccharides (AOSs), even-numbered oligosaccharides prepared from agar, are applied to various food, including supplements, drinks, and jellies because of their biological activities. This study aimed to evaluate the AOS permeation in the gastrointestinal tract in vivo and in vitro. Agarobiose (Abi), agarotetraose (Ate), and agarohexaose (Ahe) were detected in rat plasma after oral administration of AOSs. The detection level of agarobiose in the plasma was higher than that of agarohexaose, which was consistent with the permeation study using Caco-2 cell monolayers. Further, the adenosine triphosphate inhibitor (sodium azide) or endocytosis inhibitor (colchicine) did not inhibit AOS permeation through Caco-2 cell monolayers. Conversely, AOS permeation enhanced upon treatment with cytochalasin B, a tight junction disrupter, suggesting that AOSs might have passed mainly through the tight junctions between the intestinal epithelial cells. These results indicate that AOSs, especially agarobiose, can be absorbed as an intact form via the gastrointestinal tract across the intestinal epithelium through the paracellular pathway.

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Cytochalasin-B modulation of intestinal epithelial tight junction is mediated by myosin light chain kinase

Cited by 2 publications

References 0 publications

Permeation of Disaccharides Derived from Chondroitin Sulfate through Human Intestinal Caco-2 Cell Monolayersviathe Paracellular Pathway

Permeation of Disaccharides Derived from Chondroitin Sulfate through Human Intestinal Caco-2 Cell Monolayersviathe Paracellular Pathway

Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats

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