Cytochemical staining procedures selective for sarcotubular systems of muscle: Modifications and applications

Forbes, Michael S.; Plantholt, Barbara A.; Sperelakis, Nick

doi:10.1016/s0022-5320(77)80016-6

Cited by 130 publications

(64 citation statements)

References 31 publications

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“…For T-tubule staining, small fiber bundles were placed overnight in 3.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2, 4°C) containing 75 mM CaCl 2 . These specimens were then postfixed in a mixture of 2% OsO 4 and 0.8% K 3 Fe(CN) 6 for 1-2 h followed by a rinse with 0.1 M sodium cacodylate buffer with 75 mM CaCl 2 (Sommer and Waugh, 1976;Forbes et al, 1977). Samples were then dehydrated, infiltrated, and embedded as before.…”

Section: Fixation and Embedding Of Samples For Electron Microscopy (Em)mentioning

confidence: 99%

Mitochondria Are Linked to Calcium Stores in Striated Muscle by Developmentally Regulated Tethering Structures

Boncompagni¹,

Rossi

Micaroni

et al. 2009

MBoC

249

272

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Bi-directional calcium (Ca 2؉ ) signaling between mitochondria and intracellular stores (endoplasmic/sarcoplasmic reticulum) underlies important cellular functions, including oxidative ATP production. In striated muscle, this coupling is achieved by mitochondria being located adjacent to Ca 2؉ stores (sarcoplasmic reticulum [SR]) and in proximity of release sites (Ca 2؉ release units [CRUs]). However, limited information is available with regard to the mechanisms of mitochondrial-SR coupling. Using electron microscopy and electron tomography, we identified small bridges, or tethers, that link the outer mitochondrial membrane to the intracellular Ca 2؉ stores of muscle. This association is sufficiently strong that treatment with hypotonic solution results in stretching of the SR membrane in correspondence of tethers. We also show that the association of mitochondria to the SR is 1) developmentally regulated, 2) involves a progressive shift from a longitudinal clustering at birth to a specific CRU-coupled transversal orientation in adult, and 3) results in a change in the mitochondrial polarization state, as shown by confocal imaging after JC1 staining. Our results suggest that tethers 1) establish and maintain SR-mitochondrial association during postnatal maturation and in adult muscle and 2) likely provide a structural framework for bi-directional signaling between the two organelles in striated muscle.

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Section: Fixation and Embedding Of Samples For Electron Microscopy (Em)mentioning

confidence: 99%

Mitochondria Are Linked to Calcium Stores in Striated Muscle by Developmentally Regulated Tethering Structures

Boncompagni¹,

Rossi

Micaroni

et al. 2009

MBoC

249

272

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show abstract

“…In general, the peripheral SR is located close to the plasma membrane and sometimes in apposition with the caveolae (equivalent to junctional SR described above). This SR element is in contact with the deep SR positioned near the myofilaments and is in continuity with the central SR deeper within the cell and associated with the nuclear membrane (Forbes et al, 1977(Forbes et al, , 1979. Notable details of the analogy of smooth muscle SR to that of striated muscle have been painstakingly pointed out by Forbes et al (1979) who described as "peripheral SR" the collection of saccules, tubules, and cisternae lying in close apposition (gap of 10 -20 nm) to the inner plasmalemmal surface.…”

Section: Capacitative Ca 2ϩ Entrymentioning

confidence: 99%

Pharmacological Modulation of Sarcoplasmic Reticulum Function in Smooth Muscle

Laporte

Hui

Laher

2004

Pharmacological Reviews

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“…Finally, and perhaps most importantly, the limited number of selective stains available until recently for use with HVEM restricted its use. Most of the available stains were based on enzyme activity [32] or some nebulous reaction of unknown chemistry that induced the deposition of metallic precipitates within certain cells or cellular compartments [8,9]. Fortunately, recent advances in instrumentation, computational tools and specimen preparation techniques have overcome each of these problems, reinvigorating high voltage microscopy and once again placing these instruments in the forefront of biological microscopy.…”

mentioning

confidence: 99%