Cytochemical study of human lymphocytes stimulated by PHA in function of donor age

Abstract

The 3H-AM binding reflects the structural changes involved in the cellular differentiation. This parameter was studied during blastic transformation of human lymphocytes, in relation to the age of the donor. Although they are individual variations, the 3H-AM binding is higher in the young group than in the aged subjects, as well as the blast transformation score. These results indicate that the weak lymphocyte response to the mitogen lectine (PHA) stimulation could be related to some age-induced structural alterations of the chromatin, resulting in an irreversible blockage in G1, at least in some of the T lymphocytes.

Nuclear binding of tritiated actinomycin in surface epithelial cells from normal stomach and atrophic gastritis.

“…3) (Deschner et al, 1972;Willems et al, 1972 during transcription and replication (Ringertz and Bolund, 1969;Beato et al, 1970;Pederson and Robbins, 1972). It has also been shown, when resting and cycling cells are compared, that the capacity to bind 3H-AM by the nuclei is increased in the latter (Pederson, 1972;Preumont et al, 1977).…”

“…It has also been shown, when resting and cycling cells are compared, that the capacity to bind 3H-AM by the nuclei is increased in the latter (Pederson, 1972;Preumont et al, 1977).…”

Cell differentiation in human gastric gland as revealed by nuclear binding of tritiated actinomycin.

“…When resting and cycling cells are compared, the capacity to bind 3H-AM by the nuclei is higher in the latter (Pederson, 1972;Preumont et al, 1978).…”

“…A sample for routine histology was stained with haematoxylin erythrosine. The sections were then treated with 3H-actinomycin as previously described and submitted to the autoradiographic process (Brachet et al, 1969;Preumont et al, 1978). Twenty-four slides were prepared from the tissue biopsies obtained in each case.…”