Cytochrome oxidase and alternative oxidase pathways of mitochondrial electron transport chain are important for the photosynthetic performance of pea plants under salinity stress conditions

Analin, Benedict; Mohanan, Akhil; Bakka, Kavya; Challabathula, Dinakar

doi:10.1016/j.plaphy.2020.05.022

Cited by 46 publications

(18 citation statements)

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“…The production of amino acids and other organic compounds by plants during respiration leads to the production of ATP in the mitochondria and can be accompanied by the production of energy during protein synthesis ( Tcherkez et al, 2020 ). Photosynthesis and ribosome synthesis are two important metabolic processes in plants; their interaction requires the sharing of energy intermediates and is crucial for plant growth and energy metabolism ( Analin et al, 2020 ). In plants, salt stress affects the main processes of growth, such as germination, photosynthesis, nutrient transport, or production metabolism, and ultimately reduces the rate of vegetative growth ( Dinakar et al, 2010a , b ).…”

Section: Discussionmentioning

confidence: 99%

Growth, Stoichiometry, and Palatability of Suaeda salsa From Different Habitats Are Demonstrated by Differentially Expressed Proteins and Their Enriched Pathways

et al. 2021

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Suaeda salsa (L.) Pall., a medicinal and edible plant, has green and red-violet ecotypes that exhibit different phenotypes, tastes, and growth characteristics. However, few studies have focused on these differences from the aspect of differentially expressed proteins under the conditions of different habitats in the field. In this study, two ecotypes of S. salsa from the intertidal (control) and supratidal (treatment) habitats of the Yellow River Delta were selected. A total of 30 individual leaves were mixed into six samples (three biological replicates for each) and subjected to protein extraction by using tandem mass tag-labeled quantitative proteomic technology. A total of 4771 proteins were quantitated. They included 317 differentially expressed proteins (2.0-fold change, p < 0.05), among which 143 were upregulated and the remaining 174 were downregulated. These differentially expressed proteins mainly participated in biological processes, such as response to stimulus, stress, and biotic stimulus; in molecular functions, such as methyltransferase activity, transferase activity, one-C group transfer, and tetrapyrrole binding; and in cell components, such as non-membrane-bound organelles, intracellular non-membrane-bound organelles, chromosomes, and photosystems. The differentially expressed proteins were mainly enriched in eight pathways, among which the ribosome, phenylpropanoid biosynthesis, and photosynthesis pathways had higher protein numbers than the other pathways. The upregulation of differentially expressed proteins related to the ribosome and photosynthesis increased the relative growth rate and reduced the N:P ratio of S. salsa from the supratidal habitat, thereby improving its palatability. By contrast, most of the differentially expressed proteins involved in phenylpropanoid biosynthesis were downregulated in S. salsa from the intertidal habitat. This result indicated that S. salsa from the intertidal habitat might accumulate flavonoids, lignin, and other secondary metabolites in its leaves that confer a bitter taste. However, these secondary metabolites might increase the medicinal value of S. salsa from the intertidal habitat. This work could provide a theoretical basis and data support for the sustainable and high-value utilization of medicinal and edible plants from coastal wetlands.

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Section: Discussionmentioning

confidence: 99%

Growth, Stoichiometry, and Palatability of Suaeda salsa From Different Habitats Are Demonstrated by Differentially Expressed Proteins and Their Enriched Pathways

et al. 2021

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“…AOX is a nonenergy-conserving terminal oxidase in the mitochondrial ETC, and previous studies have shown that AOX functions to balance energy stability and keep the ETC flowing through mitochondria by limiting the formation of mitochondrial ROS [ 35 , 36 ]. In plants, aox plays important roles in countering abiotic stresses, including drought, light, temperature and salinity [ 32 , 69 , 70 ]. For example, in tobacco, under drought stress, the recoverability of aox knockdown plants is strongly compromised [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Alternative oxidase gene induced by nitric oxide is involved in the regulation of ROS and enhances the resistance of Pleurotus ostreatus to heat stress

et al. 2021

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Background In China, during the cultivation process of Pleurotus ostreatus, the yield and quality of fruiting bodies are easily affected by high temperatures in summer. Nitric oxide (NO) plays an important regulatory role in the response to abiotic stress, and previous studies have found that NO can induce alternative oxidase (aox) experssion in response to heat stress (HS) by regulating aconitase. However, the regulatory pathway of NO is complex, and the function and regulation of the aox gene in the response to HS remain unclear. Results In this study, we found that NO affected nicotinamide adenine dinucleotide (NADH) and adenosine triphosphate (ATP) levels, reduced hydrogen peroxide (H2O2) and superoxide anion (O2−) contents, and slowed O2− production. Further RNA-Seq results showed that NO regulated the oxidation-reduction process and oxidoreductase activity, affected the cellular respiration pathway and activated aox gene expression. The function of aox was determined by constructing overexpression (OE) and RNA interference (RNAi) strains. The results showed that the OE-aox strains exhibited obviously improved growth recovery after exposure to HS. During exposure to HS, the OE-aox strains exhibited reduced levels of NADH, the product of the tricarboxylic acid (TCA) cycle, and decreased synthesis of ATP, which reduced the production and accumulation of reactive oxygen species (ROS), whereas the RNAi-aox strains exhibited the opposite result. In addition, aox mediated the expression of antioxidant enzyme genes in the mycelia of P. ostreatus under HS through the retrograde signaling pathway. Conclusions This study shows that the expression of the aox gene in P. ostreatus mycelia can be induced by NO under HS, that it regulates the TCA cycle and cell respiration to reduce the production of ROS, and that it can mediate the retrograde signaling pathway involved in the mycelial response to HS. Graphical abstract

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“…AOX is a nonenergy-conserving terminal oxidase in the plant mitochondrial ETC, and previous studies have shown that AOX functions to balance energy stability and keep the ETC owing through mitochondria by limiting the formation of mitochondrial ROS [24,25]. The function of aox in plants has been studied in more depth than that in fungi, and these studies have shown that aox plays important roles in countering abiotic stresses, including drought, light, temperature and salinity [21,58,59]. In Medicago truncatula, high aox expression can regulate ROS, protect the light system and enhance the resistance to salt stress [20], and under drought stress, the recoverability of tobacco to aox knockdown is strongly compromised [60].…”

Section: Discussionmentioning

confidence: 99%

Alternative Oxidase Gene Induced by Nitric Oxide is Involved in the Regulation of ROS and Enhance the Resistance of Pleurotus Ostreatus to Heat Stress

Hou¹,

Zhao

Huang

et al. 2020

Preprint

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Background: Pleurotus ostreatus is easily affected by temperature during its cultivation. Nitric oxide (NO) plays an important regulatory role in the response to abiotic stress, and previous studies have found that NO can induce alternative oxidase (aox) in response to heat stress (HS) by regulating aconitase. However, the regulatory pathway of NO is complex, and the function and regulation of the aox gene in the response to HS remain unclear.Results: In this study, we found that NO affected the NADH and ATP levels, reduced the H2O2 and O2- contents, and slowed the production of O2-. Further RNA-Seq results showed that NO regulated the oxidation-reduction process and oxidoreductase activity, affected the cellular respiration pathway and activated aox gene expression. The function of aox was determined by constructing overexpression (OE) and RNA interference (RNAi) strains. The results showed that the OE-aox strains showed obvious advantages in experiencing growth recovery after exposure to HS. During exposure to HS, the OE-aox strains exhibited reduced levels of NADH, the product of the TCA cycle, and decreased synthesis of ATP, which reduced the production and accumulation of ROS, whereas the RNAi-aox strains exhibited the opposite result. In addition, aox mediated the expression of antioxidant enzyme genes in the mycelia of P. ostreatus under HS through the retrograde signaling pathway. Conclusions: This study shows that the aox gene in P. ostreatus mycelia can be induced by NO under HS, regulates the TCA cycle and cell respiration to reduce the production of ROS, and can mediate the retrograde signaling pathway involved in the mycelial response to HS.

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Cytochrome oxidase and alternative oxidase pathways of mitochondrial electron transport chain are important for the photosynthetic performance of pea plants under salinity stress conditions

Cited by 46 publications

References 46 publications

Growth, Stoichiometry, and Palatability of Suaeda salsa From Different Habitats Are Demonstrated by Differentially Expressed Proteins and Their Enriched Pathways

Growth, Stoichiometry, and Palatability of Suaeda salsa From Different Habitats Are Demonstrated by Differentially Expressed Proteins and Their Enriched Pathways

Alternative oxidase gene induced by nitric oxide is involved in the regulation of ROS and enhances the resistance of Pleurotus ostreatus to heat stress

Alternative Oxidase Gene Induced by Nitric Oxide is Involved in the Regulation of ROS and Enhance the Resistance of Pleurotus Ostreatus to Heat Stress

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