Cytochrome P450IA induction by a coplanar PCB, a PAH mixture, and PCB-contaminated sediment extracts following microinjection of rainbow trout sac-fry

Engwall, Magnus; Brunström, Björn; Brewer, Andrea; Norrgren, Leif

doi:10.1016/0166-445x(94)00047-6

Cited by 41 publications

(22 citation statements)

References 21 publications

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“…An increased number of hepatocytes and binucleate and polynucleated cells were observed in fish liver treated with 50 and 100 g sediment after 2 weeks. This agrees with results of Engwall et al (1994), who found numerous mitotic figures in liver after microinjection of an extract of PCB-contaminated sediment into rainbow trout.…”

Section: Discussionsupporting

confidence: 92%

“…They probably exert most of their toxic effects, with variable potency, through the Ah receptor (Safe 1990;Ahlborg et al 1992). One of the most sensitive biochemical responses to these compounds is the induction of specific cytochrome P450 1A1 in the liver (measured as ethoxyresorufin O-deethylase [EROD] activity) (Safe 1990;Kopponen et al 1994;Engwall et al 1994). These observations validate the use of enzyme induction in vivo and in vitro as a rapid and simple bioassay for assessing the toxic effect of dioxin-like compounds.…”

mentioning

confidence: 72%

“…In this study, a decreased number of mitochondria and deformation of cristae were in all exposed fish. This phenomenon may be a common response in fish hepatocyte to various chemical stressors (Klaasseen et al 1979;Hinton et al 1988), including PCB and PAH (Norrgren et al 1993;Engwall et al 1994).…”

Section: Discussionmentioning

confidence: 99%

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Cytological and Biochemical Alterations in Carassius auratus Hepatocytes from Exposure to Sediment Containing Dioxins and Related Compounds

Zhou

et al. 1999

Archives of Environmental Contamination and Toxicology

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Abstract. Cytological and biochemical alterations of crucial carp (Carassius auratus) hepatocytes were characterized after exposure to sediments from a lake contaminated with dioxins and other industrial chemicals. Carp were exposed in 20 L water containing 25, 50, or 100 g of contaminated sediment for 2 and 4 weeks. Ultrastructural changes in the liver were characterized by severe enlargement of hepatocytes. Alterations in the cell included formation of condensed and irregular cell nucleus, polynuclei, dispersed heterochromatin, enlargement of the nucleolus, and degeneration of the nucleus. Mitochondrial numbers were reduced and cristae were deformed. Myelin figures and lysosomes were increased, and sometimes cell organelles and cell matrix were totally lost after 4 weeks of exposure. The ultrastructural alterations were correlated with exposure time and sediment concentrations. Hepatosometic index was significantly increased in experimental groups at 2 and 4 weeks as compared with the control group. EROD enzyme activities were strongly induced in liver. A trend from rough endoplasmic reticulum (RER) to SER was observed. Our results suggest that the dioxin-like compounds bound by sediment were bioavailable to C. auratus and cause sublethal effects.

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Section: Discussionsupporting

confidence: 92%

mentioning

confidence: 72%

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Cytological and Biochemical Alterations in Carassius auratus Hepatocytes from Exposure to Sediment Containing Dioxins and Related Compounds

Zhou

et al. 1999

Archives of Environmental Contamination and Toxicology

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“…The ultrastructural "ndings by TEM of hepatocytes exposed in lower dosage TCDD for 120 days were consistent with the results obtained by Norrgren et al (1993) and Engwall et al (1994), who injected PCBs to sac-fry and examined hepatic alterations.…”

Section: Discussionsupporting

confidence: 88%

“…This conclusion is consistent with the bioaccumulation regularity of TCDD in environment. Therefore, complemented with chemical analysis, this EROD bioassay can be used for monitoring long-term toxic impact of low dosage TCDD, and possesses the advantages of rapid results and cost-e$ciency.The ultrastructural "ndings by TEM of hepatocytes exposed in lower dosage TCDD for 120 days were consistent with the results obtained by Norrgren et al (1993) and Engwall et al (1994), who injected PCBs to sac-fry and examined hepatic alterations. …”

supporting

confidence: 88%

Long-Term Toxic Impact of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on the Reproduction, Sexual Differentiation, and Development of Different Life Stages of Gobiocypris rarus and Daphnia magna

et al. 2001

Ecotoxicology and Environmental Safety

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Polychlorinated dibenzo‐p‐dioxins, dibenzofurans, biphenyls and 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin equivalents in fishes from Saginaw Bay, Michigan

Giesy

Jude

Tillitt

et al. 1997

Enviro Toxic and Chemistry

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Abstract-Concentrations of polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), polychlorinated naphthalenes (PCNs), polychlorinated dibenzothiophenes (PCDTs), and 2, 3,7,3,7, equivalents were measured in composite samples of forage fishes and several sizes of walleyes (Stizostedion vitreum) and common carp (Cyprinus carpio) collected from Saginaw Bay, Michigan, USA. The concentrations of 2,3,7,8-TCDD equivalents determined from the use of TCDD equivalency factors (TEFs) and instrumentally determined concentrations of individual congeners were designated TEq. The 2,3,7,8-TCDD equivalents determined on the same extracts with the H4IIE bioassay were defined as TCDD-EQ. Concentrations of TCDD-EQ and TEq were significantly, but poorly correlated (R 2 ϭ 0.44). Concentrations of TCDD-EQ and TEq ranged from 10.6 to 348 and 13.5 to 69.5 ng/kg, wet weight, respectively. The ratio of TCDD-EQ to TEq ranged from 0.5 to 5.0, with the greatest ratio observed in common carp but the average ratio between TEq and TCDD-EQ was approximately 1.0. In common carp, larger walleye, and medium-sized alewife (Alosa pseudoharengus) there were significant concentrations of TCDD-EQ that could not be accounted for by TEq calculated from an additive model of PCBs, PCDDs, or PCDFs. As much as 75% of the TCDD-EQ in some samples could not be accounted for by TEqs. It is unlikely that PCNs, PCDTs, or polychlorinated diphenyl ethers (PCDEs) occurred at concentrations that were, based on their relative potencies, great enough to contribute significant quantities of TCDD-EQ. The discrepency between TCDD-EQs and TEqs was not due to antagonisms among congeners or extraction efficiencies.

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Cytochrome P450IA induction by a coplanar PCB, a PAH mixture, and PCB-contaminated sediment extracts following microinjection of rainbow trout sac-fry

Cited by 41 publications

References 21 publications

Cytological and Biochemical Alterations in Carassius auratus Hepatocytes from Exposure to Sediment Containing Dioxins and Related Compounds

Cytological and Biochemical Alterations in Carassius auratus Hepatocytes from Exposure to Sediment Containing Dioxins and Related Compounds

Long-Term Toxic Impact of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on the Reproduction, Sexual Differentiation, and Development of Different Life Stages of Gobiocypris rarus and Daphnia magna

Polychlorinated dibenzo‐p‐dioxins, dibenzofurans, biphenyls and 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin equivalents in fishes from Saginaw Bay, Michigan

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