Cytocompatibility and cell migration evaluation of calcium silicate‐based root canal sealer compared to epoxide‐amine resin sealer in stem cells from human apical papilla: An in‐vitro study

Nguyen, L.; Pham, Loc Nguyen Gia; Pham, Khoa Van; Nguyen, Mai; Ngo, Lan Thi Quynh

doi:10.1111/aej.12786

Cited by 4 publications

(4 citation statements)

References 27 publications

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“…In fact, to the author’s knowledge, this is the first study on BrF and, to date, there is scarce evidence on the biological properties of AHPbcs and its immunomodulatory potential yet to be elucidated [ 25 , 27 , 30 , 47 ]. Parallelly, AHP was used as a comparison, given its extensive evidence and common use as a control in similar studies [ 26 , 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative bioactivity and immunomodulatory potential of the new Bioroot Flow and AH Plus Bioceramic sealer: An in vitro study on hPDLSCs

Sanz,

López-García,

García-Bernal

et al. 2024

Clin Oral Invest

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Objectives To evaluate the cytocompatibility, bioactivity, and anti-inflammatory potential of the new pre-mixed calcium silicate cement-based sealers Bioroot Flow (BrF) and AH Plus Bioceramic Sealer (AHPbcs) on human periodontal ligament stem cells (hPDLSCs) compared to the epoxy resin-based sealer AH Plus (AHP). Materials and methods Standardized discs and 1:1, 1:2, and 1:4 eluates of BrF, AHPbcs and AHP after setting were prepared. The following assays were performed: cell attachment and morphology via SEM, cell viability via a MTT assay, cell migration/proliferation via a wound-healing assay, cytoskeleton organization via immunofluorescence staining; cytokine release via ELISA; osteo/cemento/odontogenic marker expression via RT-qPCR, and cell mineralized nodule formation via Alizarin Red S staining. HPDLSCs were isolated from extracted third molars from healthy patients. Comparisons were made with hPDLSCs cultured in unconditioned (negative control) or osteogenic (positive control) culture media. Statistical significance was established at p < 0.05. Results Both BrF and AHPbcs showed significantly positive results in the cytocompatibility assays (cell metabolic activity, migration, attachment, morphology, and cytoskeleton organization) compared with a negative control group, while AHP showed significant negative results. BrF exhibited an upregulation of at least one osteo/cementogenic marker compared to the negative and positive control groups. BrF showed a significantly higher calcified nodule formation than AHPbcs, the negative and positive control groups, while AHPbcs was higher than the negative control group. Both were also significantly higher than AHP group. Conclusion BrF and AHPbcs exhibit adequate and comparable cytocompatibility on hPDLSCs. BrF also promoted the osteo/cementogenic differentiation of hPDLSCs. Both calcium silicate-based sealers favored the downregulation of the inflammatory cytokine IL-6 and the calcified nodule formation from hPDLSCs. BrF exerted a significantly higher influence on cell mineralization than AHPbcs. Clinical relevance This is the first study to elucidate the biological properties and immunomodulatory potential of Bioroot Flow and AH Plus Bioceramic Sealer. The results act as supporting evidence for their use in root canal treatment.

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Section: Discussionmentioning

confidence: 99%

Comparative bioactivity and immunomodulatory potential of the new Bioroot Flow and AH Plus Bioceramic sealer: An in vitro study on hPDLSCs

Sanz,

López-García,

García-Bernal

et al. 2024

Clin Oral Invest

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“…Regarding cellular migration, literature suggests that the mechanisms underlying wound closure in the scratch assay encompass both cellular proliferation and migration capabilities [ 20 ]. Indeed, the interaction between biomaterials and cellular structures can precipitate cellular degeneration and impede the process of wound healing [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, the interaction between biomaterials and cellular structures can precipitate cellular degeneration and impede the process of wound healing [ 21 ]. In the context of this study, cells treated with bioceramics exhibited responses analogous to those observed in the untreated control group throughout the duration of the wound healing assay [ 20 , 22 ]. Furthermore, an initial assessment of cytotoxicity was conducted through a morphological analysis utilizing immunofluorescence techniques.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility, bioactivity and immunomodulatory properties of three calcium silicate-based sealers: an in vitro study on hPDLSCs

Mora,

García-Bernal,

Rodríguez-Lozano

et al. 2024

Clin Oral Invest

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Objectives To assess the biocompatibility, bioactivity, and immunomodulatory properties of three new calcium silicate cement-based sealers: Ceraseal (CS), Totalfill BC Sealer (TFbc) and WellRoot ST (WR-ST) on human periodontal ligament stem cells (hPDLSCs). Materials and methods HPDLSCs were isolated from extracted third molars from healthy patients. Eluates (1:1, 1:2, and 1:4 ratio) and sample discs of CS, TFbc and WR-ST after setting were prepared. A series of assays were performed: cell characterization, cell metabolic activity (MTT assay) cell attachment and morphology (SEM assay), cell migration (wound-healing assay), cytoskeleton organization (phaloidin-based assay); IL-6 and IL-8 release (ELISA); differentiation marker expression (RT-qPCR assay), and cell mineralization (Alizarin Red S staining). HPDLSCs cultured in unconditioned (negative control) or osteogenic (positive control) culture media were used as a comparison. Statistical significance was established at p < 0.05. Results All the tested sealers exhibited similar results in the cytocompatibility assays (cell metabolic activity, migration, attachment, morphology, and cytoskeleton organization) compared with a negative control group. CS and TFbc exhibited an upregulation of at least one osteo/cementogenic marker compared to the negative and positive control groups. CS and TFbc also showed a significantly higher calcified nodule formation than the negative and positive control groups. Both the marker expression and calcified nodule formation were significantly higher in CS-treated cells than TFbc treated cells. WR-ST exhibited similar results to the control group. CS and TFbc-treated cells exhibited a significant downregulation of IL-6 after 72 h of culture compared to the negative control group (p < 0.05). Conclusion All the tested sealers exhibited an adequate cytocompatibility. CS significantly enhances cell differentiation by upregulating the expression of key genes associated with bone and cementum formation. Additionally, CS was observed to facilitate the mineralization of the extracellular matrix effectively. In contrast, the effects of TFbc and WR-ST on these processes were less pronounced compared to CS. Furthermore, both CS and TFbc exhibited an anti-inflammatory potential, contributing to their potential therapeutic benefits in regenerative endodontics. Clinical relevance This is the first study to compare the biological properties and immunomodulatory potential of Ceraseal, Totalfill BC Sealer, and WellRoot ST. The results act as supporting evidence for their use in root canal treatment.

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“…It has been described that the wound healing closure in the scratch assay involves proliferation and migration capacity (Nguyen et al, 2023). In fact, biological interactions between biomaterials and cells can cause cellular degeneration and delay wound healing (Sanz et al, 2021).…”

Section: Alizarin Red Stainingmentioning

confidence: 99%

Premixed calcium silicate‐based ceramic sealers promote osteogenic/cementogenic differentiation of human periodontal ligament stem cells: A microscopy study

López‐García,

Sánchez‐Bautista,

García‐Bernal

et al. 2024

Microscopy Res & Technique

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To evaluate the effects of premixed calcium silicate based ceramic sealers on the viability and osteogenic/cementogenic differentiation of human periodontal ligament stem cells (hPDLSCs). The materials evaluated were TotalFill BC Sealer (TFbc), AH Plus Bioceramic Sealer (AHPbc), and Neosealer Flo (Neo). Standardized discs and 1:1, 1:2, and 1:4 eluates of the tested materials were prepared. The following in vitro experiments were carried out: ion release, cell metabolic activity 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, cell migration, immunofluorescence experiment, cell attachment, gene expression, and mineralization assay. Statistical analyses were performed using one‐way ANOVA followed by Tukey's post hoc test (p < .05). Increased Ca2+ release was detected in TFbc compared to AHPbc and Neo (*p < .05). Biological assays showed a discrete cell metabolic activity and cell migration in Neo‐treated cell, whereas scanning electronic microscopy assay exhibited that TFbc group had a better cell adhesion process of substrate attachment, spreading, and cytoskeleton development on the niche‐like structures of the cement than AHPbc and Neo. The sealers tested were able to induce overexpression of the CEMP‐1, ALP, and COL1A1 genes in the first days of exposure, particularly in the case of TFbc (***p < .001). All materials tested significantly increased the mineralization of hPDLSCs when compared to the negative control, although more pronounced calcium deposition was observed in the TFbc‐treated cells (***p < .001). Our results suggested that TFbc promotes cell differentiation, both by increasing the expression of key osteo/odontogenic genes and by promoting mineralization of the extracellular matrix, whereas this phenomenon was less evident in Neo and AHPbc.Research Highlights TFbc group had a better cell adhesion process of substrate attachment, spreading, and cytoskeleton development on the niche‐like structures of the cement than AHPbc and Neo. The sealers tested were able to induce overexpression of the CEMP‐1, ALP, and COL1A1 genes in the first days of exposure, particularly in the case of TFbc. All materials tested significantly increased the mineralization of hPDLSCs when compared to the negative control, although more pronounced calcium deposition was observed in the TFbc‐treated cells.

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Cytocompatibility and cell migration evaluation of calcium silicate‐based root canal sealer compared to epoxide‐amine resin sealer in stem cells from human apical papilla: An in‐vitro study

Cited by 4 publications

References 27 publications

Comparative bioactivity and immunomodulatory potential of the new Bioroot Flow and AH Plus Bioceramic sealer: An in vitro study on hPDLSCs

Comparative bioactivity and immunomodulatory potential of the new Bioroot Flow and AH Plus Bioceramic sealer: An in vitro study on hPDLSCs

Biocompatibility, bioactivity and immunomodulatory properties of three calcium silicate-based sealers: an in vitro study on hPDLSCs

Premixed calcium silicate‐based ceramic sealers promote osteogenic/cementogenic differentiation of human periodontal ligament stem cells: A microscopy study

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