Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres

Abstract

Aim: This study was performed to evaluate the biocompatibility of pure titanium and Ti-6Al-4V metals coated with hydroxyapatite (HA) by plasma spray using different plasma gas atmospheres. Materials and methods:The cell viabilities for each HA-coated sample in an atmosphere of argon, argon-hydrogen, nitrogen, and nitrogen-hydrogen were studied using MTT assay and platelet adhesion test. Results:The mean cell viabilities by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay of samples coated with HA in argon-hydrogen plasma atmosphere showed maximum cell viability at different time intervals compared with other coating atmospheres of argon-hydrogen, nitrogen, and nitrogen-hydrogen. A statistically significant value of cell viability (p < 0.001) was observed between and within the groups of argon, argon-hydrogen, nitrogen, and nitrogen-hydrogen plasma gas atmosphere. The platelet adhesion study showed agglomerates of platelet cells in some isolated regions of HA for all atmospheres. Significance:The results obtained in this study can serve as a guide for the development of new Ti-based HA-coated implants in different plasma gas atmospheres.