Cytogenetic analysis on eight human breast tumor cell lines: High frequencies of 1q, 11q and HeLa-like marker chromosomes

Satya-Prakash, K. L.; Pathak, Sen; Hsü, T. C.; Olivé, Matilde; Cailleau, Relda

doi:10.1016/0165-4608(81)90057-1

Cited by 61 publications

(27 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The deletion of ErbB2 in MCF-7 cells (Szö llö si et al 1995;Shadeo and Lam 2006) and the normal ErbB2 copy number in MDA-MB-231 cells (Satya-Prakash et al 1981;Grushko et al 2002;Lottner et al 2005) were also previously documented and are in keeping with our data. However, no data showing the TOP2A status in MCF-7 and MDA-MB-231 cells were previously reported.…”

Section: Discussionsupporting

confidence: 80%

Phenotypic and Genetic Characterization of Circulating Tumor Cells by Combining Immunomagnetic Selection and FICTION Techniques

Campos

Prior²,

Warleta

et al. 2008

J Histochem Cytochem.

View full text Add to dashboard Cite

S U M M A R Y The presence of circulating tumor cells (CTCs) in breast cancer patients has been proven to have clinical relevance. Cytogenetic characterization of these cells could have crucial relevance for targeted cancer therapies. We developed a method that combines an immunomagnetic selection of CTCs from peripheral blood with the fluorescence immunophenotyping and interphase cytogenetics as a tool for investigation of neoplasm (FICTION) technique. Briefly, peripheral blood (10 ml) from healthy donors was spiked with a predetermined number of human breast cancer cells. Nucleated cells were separated by double density gradient centrifugation of blood samples. Tumor cells (TCs) were immunomagnetically isolated with an anti-cytokeratin antibody and placed onto slides for FICTION analysis. For immunophenotyping and genetic characterization of TCs, a mixture of primary monoclonal anti-pancytokeratin antibodies was used, followed by fluorescent secondary antibodies, and finally hybridized with a TOP2A/HER-2/CEP17 multicolor probe. Our results show that TCs can be efficiently isolated from peripheral blood and characterized by FICTION. Because genetic amplification of TOP2A and ErbB2 (HER-2) in breast cancer correlates with response to anthracyclines and herceptin therapies, respectively, this novel methodology could be useful for a better classification of patients according to the genetic alterations of CTCs and for the application of targeted therapies. (J Histochem Cytochem 56:667-675, 2008)

show abstract

Section: Discussionsupporting

confidence: 80%

Phenotypic and Genetic Characterization of Circulating Tumor Cells by Combining Immunomagnetic Selection and FICTION Techniques

Campos

Prior²,

Warleta

et al. 2008

J Histochem Cytochem.

View full text Add to dashboard Cite

show abstract

“…The karyotypes deduced by reverse painting agreed to some extent with those by banding analysis published previously (Engel et al, 1978;Keydar et al, 1979;Satya-Prakash et al, 1981). Since other aspects of the cell lines, including their morphology and the mode and range of chromosomes, generally agreed with published data, the karyotype discrepancies can be attributed to misinterpretations of chromosome identity by banding analysis.…”

Section: Comparison With Banded Karyotypessupporting

confidence: 81%

“…The breast carcinoma cell lines T-47D (Keydar et al, 1979), MDA-MB-361 (Satya-Prakash et al, 1981), and ZR-75-1 (Engel et al, 1978) …”

Section: Cell Culturementioning

confidence: 99%

Cytogenetic analysis of three breast carcinoma cell lines using reverse chromosome painting

Morris

Carter

Ferguson‐Smith

et al. 1997

Genes Chromosom. Cancer

View full text Add to dashboard Cite

“…While in general the cultured samples do reflect the same 'categories' of chromosome change found in the uncultured samples (6-9 and Figure 2), karyotypic selection unquestionably can and does occur in vitro (10,11). Summarizing from unpublished cases from my own laboratory, as well as several recent reports utilizing chromosome banding (12)(13)(14)(15), primary and metastatic breast tumors invariably demonstrate structural chromosome alterations and usually demonstrate marker chromosomes.…”

Section: General Commentsmentioning

confidence: 89%

Cytogenetic and molecular biologic alterations in human breast cancer: A review

Trent

1985

Breast Cancer Res Tr

View full text Add to dashboard Cite

show abstract

Cytogenetic analysis on eight human breast tumor cell lines: High frequencies of 1q, 11q and HeLa-like marker chromosomes

Cited by 61 publications

References 24 publications

Phenotypic and Genetic Characterization of Circulating Tumor Cells by Combining Immunomagnetic Selection and FICTION Techniques

Phenotypic and Genetic Characterization of Circulating Tumor Cells by Combining Immunomagnetic Selection and FICTION Techniques

Cytogenetic analysis of three breast carcinoma cell lines using reverse chromosome painting

Cytogenetic and molecular biologic alterations in human breast cancer: A review

Contact Info

Product

Resources

About