Cytogenetic and oxidative alterations after exposure of cultured human whole blood cells to lithium metaborate dehydrate

Çelikezen, Fatih Çağlar; Toğar, Başak; Özgeriş, Fatma Betül; İzgi̇, Mehmet Sait; Türkez, Hasan

doi:10.1007/s10616-014-9833-x

Cited by 17 publications

(8 citation statements)

References 44 publications

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“…The obtained data showed that there was no significant increase in MN rates in erythrocytes exposed to COL as compared to control values. These findings are consistent with previous evaluations (Celikezen, Turkez, Togar, & Izgi, 2014;Celikezen et al, 2016). At the end of the study, it was determined that COL had no geno-toxic damage potential.…”

Section: Micronucleus In Zebrafish Erythrocytessupporting

confidence: 91%

“…Alterations in enzyme activity and DNA damage are widely used as important biomarkers to reduce the geno-toxicity and oxidative stress of natural and / or man-made chemical materials (Celikezen, Toğar, Özgeriş, Izgi, & Türkez, 2016). Increased levels of MDA and MPO obtained at the highest dose of COL (20 mg/l) indicated the occurance of oxidative stress.…”

Section: Dna Damage Grade and Apoptosis Levelmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative and DNA Damage Potential of Colemanite on Zebrafish: Brain, Liver and Blood

Alak¹,

Parlak²,

Uçar³

et al. 2020

Turkish Journal of Fisheries and Aquatic Sciences

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Recently, boron has been used in animal feeding due to its significant biological roles. In this study, the action mechanism of colemanite (COL), a commercially important borate mineral, was aimed to investigate via evaluating parameters related to oxidative alterations on the brain, liver and blood tissues of zebrafish. For this purpose, zebrafish were exposed to different doses of COL (5, 10 and 20 mg/L) in a static test apparatus for 96 hours. Multiple biochemical analysis including determination of DNA damage (8-OHdG), apoptosis (Caspase-3), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), myeloperoxidase (MPO), paraoxonase (PON), arylesterase (AR) and lipid peroxidation (MDA) levels were performed in brain and liver tissues for assessing oxidative responses. In addition to micronucleus (MN) assay was performed in obtained blood tissues. The results indicated that low doses of COL supported antioxidant system and did not lead to oxidative stress in zebrafish brain and liver. Again, our results showed colemanite did not cause DNA damage or apoptosis at all tested concentrations. Besides the statistically insignificant changes (P>0.05) of MN rates of erythrocytes between the control and experimental groups revealed the non-genotoxic feature of COL on zebrafish. In conclusion, boron compounds especially COL can be used safely and provide positive impacts on aquatic environments.

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Section: Micronucleus In Zebrafish Erythrocytessupporting

confidence: 91%

Section: Dna Damage Grade and Apoptosis Levelmentioning

confidence: 99%

Oxidative and DNA Damage Potential of Colemanite on Zebrafish: Brain, Liver and Blood

Alak¹,

Parlak²,

Uçar³

et al. 2020

Turkish Journal of Fisheries and Aquatic Sciences

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“…Currently, more and more research on the therapeutic effect of the borax, mainly including anti-tumor effect and affecting the tumor growth, development and metastasis [26,27]. A great number of studies have concentrated on the application of borax for tumor prevention and illustrated a strong inverse correlation between borax and various types of cancer, including lung cancer, cervical cancer, prostate cancer and hepatocellular carcinoma (HCC) [2,8,9]. But, the underlying mechanisms of anti-tumor effect remain unidentified and borax administered altered the expression of miRNAs in HepG2 cells has been reported less.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, it demonstrated that those alterations were ascribed to certain cellular toxicities and genetic defects by borax. In recent years, numerous studies have focused on the application of borax for various types of cancer prevention, including liver cancer, prostate cancer, cervical cancer, and lung cancer [2,8,9]. Our previous results revealed that HepG2 cell proliferation was inhibited following 24-h borax (4 mM) treatment and demonstrated borax-induced apoptotic in a concentration-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA expression profiling involved in Borax-induced anti-tumor effect using gene-chip analysis

Wei

Zhou

et al. 2020

Preprint

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Background Borax, a boron compound, which is becoming widely recognized for its biological effects, including antioxidant activity, cytotoxicity, and potential therapeutic benefits. However, the specific molecular mechanisms underlying borax-induced anti-tumor effect still remain to be to further elucidated. MicroRNAs (miRNAs) may play key roles in cellular processes including tumor progression, cell apoptosis and cytotoxicity. Thus, this study aimed to investigate, whether miRNAs were involved in the borax-mediated anti-tumor effect using miRNA profiling of a human liver cancer cell line (HepG2) using gene-chip analysis.Methods Total RNA was extracted and purified from HepG2 cells that were treated with 4 mM borax for either 2 or 24 h. The samples underwent microarray analysis using an Agilent Human miRNA Array. Differentially expressed miRNAs were analysed by volcano plot and heatmap, and were validated using real-time fluorescent quantitative PCR (qPCR).ResultsAmong this, 2- or 24-h exposure to borax significantly altered the expression level of miRNAs in HepG2 cells, 4 or 14 were upregulated and 3 were downregulated compared with the control group, respectively (≥2-fold; P<0.05). GO enrichment analysis and KEGG pathway enrichment analysis revealed that target genes of differentially expressed miRNAs in HepG2 cells predominantly participated in MAPK signaling pathway, TGF-beta signaling pathway, NF-kappa B signaling pathway, etc; in 2-h borax treatment group, while Ras signaling pathway, FoxO signaling pathway, Cellular senescence, etc; involved in 24-h treatment group.Conclusions Result indicates that borax-induced anti-tumor effect may be associated with alterations in miRNAs.

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“…Thus, certain cellular toxicities indicated that those alterations were ascribed to genetic defects caused by borax in humans (14). Notably, it has been recently identified that borax treatment enhanced the resistance of DNA to titanium dioxide-induced damage (15). Taken together, numerous studies have focused on the application of borax for tumor prevention and demonstrated a strong inverse correlation between borax and various types of cancer, including prostate cancer, lung cancer, cervical cancer and hepatocellular carcinoma (HCC) (6–15).…”

Section: Introductionmentioning

confidence: 99%

Gene expression alterations of human liver cancer cells following borax exposure

Ying

Zhou

et al. 2019

Oncol Rep

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Borax is a boron compound that is becoming widely recognized for its biological effects, including lipid peroxidation, cytotoxicity, genotoxicity, antioxidant activity and potential therapeutic benefits. However, it remains unknown whether exposure of human liver cancer (HepG2) cells to borax affects the gene expression of these cells. HepG2 cells were treated with 4 mM borax for either 2 or 24 h. Gene expression analysis was performed using Affymetrix GeneChip Human Gene 2.0 ST Arrays, which was followed by gene ontology analysis and pathway analysis. The clustering result was validated using reverse transcription-quantitative polymerase chain reaction. A cell proliferation assay was performed using Celigo Image Cytometer Instrumentation. Following this, 2- or 24-h exposure to borax significantly altered the expression level of a number of genes in HepG2 cells, specifically 530 genes (384 upregulated and 146 downregulated) or 1,763 genes (1,044 upregulated and 719 downregulated) compared with the control group, respectively (≥2-fold; P<0.05). Twenty downregulated genes were abundantly expressed in HepG2 cells under normal conditions. Furthermore, the growth of HepG2 cells was inhibited through the downregulation of PRUNE1, NBPF1, PPcaspase-1, UPF2 and MBTPS1 (≥1.5-fold, P<0.05). The dysregulated genes potentially serve important roles in various biological processes, including the inflammation response, stress response, cellular growth, proliferation, apoptosis and tumorigenesis/oncolysis.

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Cytogenetic and oxidative alterations after exposure of cultured human whole blood cells to lithium metaborate dehydrate

Cited by 17 publications

References 44 publications

Oxidative and DNA Damage Potential of Colemanite on Zebrafish: Brain, Liver and Blood

Oxidative and DNA Damage Potential of Colemanite on Zebrafish: Brain, Liver and Blood

MicroRNA expression profiling involved in Borax-induced anti-tumor effect using gene-chip analysis

Gene expression alterations of human liver cancer cells following borax exposure

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