Cytogenetic Studies in Coffea L. and Psilanthus Hook.f. Using OMA/DAPI and FISH

Lombello, Ricardo A.; Pinto-Maglio, Cecília Alzira Ferreira

doi:10.1508/cytologia.69.85

Cited by 15 publications

(10 citation statements)

References 20 publications

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“…Allium commutatum (Beserdorfer et al 2002) and Rhoeo spathaceae (Golczyk 2011), that GC-rich heterochromatin is associated with NOR active sites. The silver staining NOR-bands observed in the four coffee species analyzed may also be associated with positive CMA 3 bands and FISH hybridization signals reported previously by Lombello and Pinto-Maglio (2004a, 2004b, 2004c for the same species.…”

Section: Discussionsupporting

confidence: 81%

“…C. congensis, C. eugenioides and C. liberica showed only one pair of chromosomes following silver impregnation, which may be chromosome number 3 as detected in C. canephora and C. dewevrei (Pierozzi et al 1999). Pinto-Maglio and Cruz (1987) and Lombello and Pinto-Maglio (2004a, 2004b, 2004c) also reported one pair of nucleolar chromosome after in situ hybridization for these species. However, C. stenophylla presented two pairs of chromosomes associated with NOR activity after silver Means followed by the same letter = differences were not significant at the level of 5% after the Tukey test.…”

Section: Discussionmentioning

confidence: 83%

“…Bouharmont (1959Bouharmont ( , 1963, Pinto-Maglio and Cruz (1998), Campa et al (2003), Lombello and Pinto-Maglio (2004a, 2004b, 2004c, Hamon et al (2009) and Razafinarivo et al (2012). The studies regarding the total amount of DNA showed similarities between C. congensis and C. liberica (1.53 pg/1.54 pg and 1.59 pg, respectively) and also between C. eugenioides and C. stenophylla (1.36 pg/1.45 pg and 1.28 pg, respectively), despite the differences between these two pairs of species (Cros et al 1994;Noirot et al 2003;Razafinarivo et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…The wild and cultivated coffee species (Coffea L., Rubiaceae family) have been extensively studied over the last 70 years, with different approaches, including molecular cytogenetics (Lombello and Pinto-Maglio 2004a, 2004b, 2004c, to improve species characterization, to establish feasible evolutionary trends in the genus, and also to investigate the origin of the wellknown polyploid C. arabica (2n = 44 chromosomes), the most important commercially available coffee.…”

Section: Introductionmentioning

confidence: 99%

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Orcein, C- and NOR-banding on mitotic chromosomes of some wild diploid coffee species (CoffeaL.)

Pierozzi

2013

Caryologia

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The mitotic chromosomes of four diploid and auto-incompatible coffee species -Coffea congensis, C. eugenioides, C. liberica and C. stenophylla have been comparatively analyzed by acetic orcein, C-banding and nucleolar organizer region (NOR)-banding techniques in order to complete the chromosome characterization in the Coffea genus, by determining morphometric parameters and heterochromatin localization, and to establish karyotype traits, including the degree of symmetry/asymmetry of the karyotypes of the four studied species. The subject is of practical interest because botanical classification of coffee species is still controversial and the reported results can contribute to a better comprehension of interspecific relationships as well as of the evolutionary trends in Coffea. Some species differed from others based on some karyomorphometric parameters. The karyotype formula obtained was the same for all coffee species analyzed, 3m + 6sm + 2sm s . All species displayed a moderate karyotype asymmetry and according to the Stebbins system, C. congensis and C. liberica were classified as 2A while C. eugenioides and C. stenophylla, classified as 2B, were more asymmetrical than the two former ones. Among the four indices used to assess karyotype asymmetry, Stebbins and Paszko AI indices were the best suited to individualize the species. C-bands occurred preferentially at pericentromeric/ centromeric position. Two pairs of chromosomes with secondary constriction and satellite segments were observed in the species following acetic orcein staining. C. congensis, C. eugenioides and C. liberica displayed one pair of chromosomes with the NOR-band after silver staining procedure, while C. stenophylla showed two pairs. Data on morphometric chromosome and NOR-banding results were suitable for the characterization of the species.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Orcein, C- and NOR-banding on mitotic chromosomes of some wild diploid coffee species (CoffeaL.)

Pierozzi

2013

Caryologia

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“…Observations at the meiotic pachytene phase provided a significantly better chromosomal characterization and allowed the identification of most bivalents of C. arabica (Pinto-Maglio and Cruz 1998). Characterization of the longitudinal differentiation of the mitotic chromosomes of coffee has progressed using other techniques such as fluorescent banding (Lombello and Pinto-Maglio 2004). In addition, the fluorescence in situ hybridization (FISH) method that opened up new perspectives.…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Genomics of Coffee One of the World’s Largest Traded Commodities

Lebailly

Andrade

Etienne

Genomics of Tropical Crop Plants

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Coffee is one of the world's most valuable agricultural export commodities. In particular, coffee is a key export and cash crop in numerous tropical and subtropical countries having a generally favorable impact on the social and physical environment. While coffee species belong to the Rubiaceae family, one of the largest tropical angiosperm families, commercial production relies mainly on two species, Coffea arabica L. and Coffea canephora Pierre, known as Robusta. Although a considerable genetic diversity is potentially available, coffee breeding is still a long and difficult process. Nevertheless, genomic approaches offer feasible strategies to decipher the genetic and molecular bases of important biological traits in coffee tree species that are relevant to the growers, processors, and consumers. This knowledge is fundamental to allow efficient use and preservation of coffee genetic resources for the development of improved cultivars in terms of quality and reduced economic and environmental costs. This review focuses on the recent progress of coffee genomics in relation to crop improvement.

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Protocols for Chromosome Preparations: Molecular Cytogenetics and Studying Genome Organization in Coffee

Li,

Schwarzacher,

Tomaszewska

et al. 2023

Mutation Breeding in Coffee With Special Reference to Leaf Rust

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Cytological preparations from cell nuclei are required to count the number of chromosomes (including determining ploidy or aneuploidy), to investigate their morphology and organization. The results are valuable for genetic and evolutionary studies, and in breeding programs to understand species relationships, polyploidy, and potential introgression of chromosomes in hybrids between different species. Preparation of good chromosome spreads with well-separated metaphase chromosomes is the foundation of cytogenetic research including chromosomal mapping based on FISH (fluorescence in situ hybridization). FISH combined with specific locus probes correlated with molecular markers to specific chromosomes for integrating physical and linkage maps as well as studying the genetic evolution of allopolyploidization, has rarely been applied in Coffea spp. despite being a global high-value crop. Cytogenetic studies of Coffea are limited by the small size and similar morphology of the chromosomes, but FISH can help to map sequences to chromosome arms and identify individual chromosomes. This chapter presents protocols for germinating seeds and growing coffee plants involving pre-treatment and fixation of root-tips where the meristems of actively growing roots have many divisions. Mitotic metaphase chromosome preparation on microscope slides is described, as well as preparing probes of 5S and 18S rDNA to be used for FISH. The FISH experiments involve a two-step protocol with pre-treatments and setting up the hybridization on day 1 and the detection of probe sites on day 2 after overnight hybridization. A final section gives advice about visualization using a fluorescent microscope and capturing images.

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Cytogenetic Studies in Coffea L. and Psilanthus Hook.f. Using OMA/DAPI and FISH

Cited by 15 publications

References 20 publications

Orcein, C- and NOR-banding on mitotic chromosomes of some wild diploid coffee species (CoffeaL.)

Orcein, C- and NOR-banding on mitotic chromosomes of some wild diploid coffee species (CoffeaL.)

Genomics of Coffee One of the World’s Largest Traded Commodities

Protocols for Chromosome Preparations: Molecular Cytogenetics and Studying Genome Organization in Coffee

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