Cytogenetic study in cultured human lymphocytes treated with three commonly used preservatives

Mpountoukas, Panagiotis; Vantarakis, Apostolos; Sivridis, Efthimios; Lialiaris, Theodoros

doi:10.1016/j.fct.2008.03.021

Cited by 89 publications

(69 citation statements)

References 27 publications

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“…Mukherjee et al (1988) revealed that sorbic acid increased SCEs and frequency of MN at the highest dose (150 mg/kg body weight) in bone marrow cells of mice. Similar results about damaging effects of sorbic acid salts potassium sorbate were also obtained by Mpountoukas et al (2008) and by Mamur et al (2010) in human lymphocytes in vitro. Some authors compared the genotoxic effect of sorbic acid and its sodium and potassium salts.…”

Section: Introductionsupporting

confidence: 81%

Genotoxicity of food preservative sodium sorbate in human lymphocytes in vitro

et al. 2012

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Section: Introductionsupporting

confidence: 81%

Genotoxicity of food preservative sodium sorbate in human lymphocytes in vitro

et al. 2012

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“…The most common aberrations are chromatid breaks which indicates benzoic acid caused DNA double strand breaks and sister chromatid union which is the breakage followed by reunion of both sister chromatids at an identical site (Murli 2003). There are many studies that showed the genotoxicity of different food additives in different cell lines (Macioszek and Kononowicz 2004;Sarıkaya and Ç akır 2005;Yılmaz et al 2008;Mpountoukas et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…A food additive is any substance or mixture of substances, other than basic food components, added to food in a scientifically controlled amount (Mpountoukas et al 2008). These additives are used widely for various purposes, including preservation, coloring and sweetening.…”

Section: Introductionmentioning

confidence: 99%

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

2009

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The chromosomal aberration (CA), sister chromatid exchange (SCE) and micronucleus test (MN) were employed to investigate the in vitro effect of antimicrobial food additive benzoic acid on human chromosomes. Lymphocytes were incubated with various concentrations (50, 100, 200 and 500 lg/ mL) of benzoic acid. The results of used assays showed that benzoic acid significantly increased the chromosomal aberration, sister chromatid exchange and micronucleus frequency (200 and 500 lg/mL) without changing the pH of the medium in a dosedependent manner. Also this additive significantly decreased the mitotic index (MI) at the highest concentration for 24 h and 100, 200 and 500 lg/mL for 48 h. This decrease was dose-dependent as well. However, it did not effect the replication (RI) and nuclear division (NDI) indices.

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“…Sister chromatid exchanges are indicators of a pre-existing DNA damage, remaining unrepaired during the duration of both cell cycles required for the incorporation of BrdU in vitro. Consequently, SCEs assessment is a sensitive and efficient method for evaluating the mutagenic action of various substances (Mpountoukas et al 2008;Lialiaris et al 2009b). …”

Section: Discussionmentioning

confidence: 99%

The cytogenetic action of ifosfamide, mesna, and their combination on peripheral rabbit lymphocytes: an in vivo/in vitro cytogenetic study

et al. 2013

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Ifosfamide (IFO) is an alkylating nitrogen mustard, administrated as an antineoplasmic agent. It is characterized by its intense urotoxic action, leading to hemorrhagic cystitis. This side effect of IFO raises the requirement for the co-administration with sodium 2-sulfanylethanesulfonate (Mesna) aiming to avoid or minimize this effect. IFO and Mesna were administrated separately on rabbit's lymphocytes in vivo, which were later developed in vitro. Cytogenetic markers for sister chromatid exchanges (SCEs), proliferation rate index (PRI) and Mitotic Index were recorded. Mesna's action, in conjunction with IFO reduces the frequency of SCEs, in comparison with the SCEs recordings obtained when IFO is administered alone. In addition to this, when high concentrations of Mesna were administered alone significant reductions of the PRI were noted, than with IFO acting at the same concentration on the lymphocytes. Mesna significantly reduces IFO's genotoxicity, while when administered in high concentrations it acts in an inhibitory fashion on the cytostatic action of the drug.

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Cytogenetic study in cultured human lymphocytes treated with three commonly used preservatives

Cited by 89 publications

References 27 publications

Genotoxicity of food preservative sodium sorbate in human lymphocytes in vitro

Genotoxicity of food preservative sodium sorbate in human lymphocytes in vitro

The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes

The cytogenetic action of ifosfamide, mesna, and their combination on peripheral rabbit lymphocytes: an in vivo/in vitro cytogenetic study

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