Cytogenetic variation in rye regenerated plants and their progeny

Abstract

LINACERO, R., and VAZQUEZ, A. M. 1992. Cytogenetic variation in rye regenerated plants and their progeny. Genome, 35: 428-430. Regenerated plants obtained from immature embryo-derived.talluses of four cultivars of rye, as well as their progeny, were cytologically analyzed. Chromosome number modified plants were found among the regenerants. The progeny of apparently normal diploid regenerated plants was tested and in some cases chromosomally abnormal plants appeared. Equally, meiotic abnormalities were observed in some of the regenerated plants and their progenies. The cytological variations observed were inheritable and in some cases both types of abnormalities could be related. The nature of the variations is discussed. Key words: Secale cereale, somaclonal variation, nondiploid plants, meiotic abnormalities. LINACERO, R., et VAZQUEZ, A. M. 1992. Cytogenetic variation in rye regenerated plants and their progeny. Genome, 35 : 428-430. Des plantes rCgCnCrCes, obtenues d'embryons immatures issus de cals de quatre cultivars de seigle, ont Ct C analysCes cytologiquement, ainsi que leurs descendants. Des plantes modifiCes quant au nombre de chromosomes ont Ct C trouvCes parmi les rCgCnCrants. Les descendants de plantes rCgCnCrCes, d'apparence diploi'de normale, ont Ct C testes et certaines d'entre eux se sont avCrCs anormaux sur le plan chromosomique. Des anormalitks mCiotjques ont aussi Ct C observCes chez certaines des plantes rCgCnCrCes, ainsi que chez leurs descendants. Ces variations cytologiques ont Ct C hCrCditaires et, dans certains cas, les deux types d'anormalitks ont pu Etre relies. La nature des variations est discutCe.

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

“…For detailed description of the abbreviation see Fig 2. Materials with the same small letter (indicated on graph) of the given set belong to the same group (Miguel and Marum 2011). Such stress may cause the regeneration of the off-type plants that could be detected at the morphological (Li et al 2010), cytological (Linacero and Vazquez 1992), genetic or epigenetic level (Bednarek et al 2007;Vining et al 2013). Some of the changes may subsequently arise among progeny originated from tissue culture-derived regenerants even after many generative cycles (Bregitzer et al 1998;Kirikovich et al 2003).…”

“…Tissue culture induced variation (TCIV) in regenerants (Li et al 2007;González et al 2013) and somaclonal variation (SV) in progeny of such regenerants (Linacero and Vazquez 1992;Ivanov et al 1998) have been observed in crops. TCIV may be manifested at the morphological level of regenerants (Jaligot et al 2000).…”

Cytological Investigation of R1 and R2-Generations of Spontaneously and Colchicine Induced Diploid Anther Derived Wheat Plants.

“…Culture conditions may increase sensitivity of heterochromatic regions in regenerated DH plants for such breaks (Youssef et al 1989). Similar and other chromosomal structural modifications has been mentioned in anther derived plants of many crops such as barley (Mix et al 1978), rice (Mercy andZapata 1986, Mezencev et al 1995), maize (Ting 1985, Lee andPhillips 1987), rye (Linacero and Vazquez 1992) and cabbage (Chiang et al 1989). Plants carrying chromosomal abnormalities are a main source of male-sterility and cytological instability which usually impact the whole breeding program.…”

Effect of genotype on chromosome variation in tissue culture of inbred and outbred rye

“…Chromosome breakage in regenerants of cv. Ailés has been reported previously (Linacero & Vazquez, 1992).…”

“…There have been previous studies describing somaclonal variation in 'standard' outbred rye (e.g. Linacero & Vazquez, 1992). The aim of our study was not to make any direct comparisons between the inbred and outbred lines, but to investigate how the genetic make-up of the donor material influences the outcome in terms of somaclonal variation released.…”