Cytokeratin 19 expression in early oral squamous cell carcinoma and their metastasis: Inadequate biomarker for one-step nucleic acid amplification implementation in sentinel lymph node biopsy procedure

Noorlag, Rob; Bree, Remco de; Willems, Stefan M.

doi:10.1002/hed.24847

Cited by 14 publications

(6 citation statements)

References 13 publications

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“…Most of the negative OSNA/positive HES were due to an allocation bias, only two of them were due to non-expression of CK19 mRNA. As found in our study, CK19 protein expression in HNSCC primary tumor range from 39% to 80% [11,18,21]. Some authors suggested to restrain OSNA analysis to patient harboring a tumor positive for CK19 IHC [11,18] excluding tumor with no CK19 protein expression.…”

Section: Discussionsupporting

confidence: 64%

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One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma

Peigné¹,

Godey

Gallo

et al. 2020

Oral Oncology

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Section: Discussionsupporting

confidence: 64%

“…Furthermore, Norlaag et al reported that 36 to 43% of CK19 negative tumor have CK19 positive metastatic LN for [21]. This underline that CK19 protein expression in the primary tumor is not well correlated to the one of metastatic LN.…”

Section: Discussionmentioning

confidence: 99%

One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma

Peigné¹,

Godey

Gallo

et al. 2020

Oral Oncology

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“…However, some carcinomas in the process of carcinogenesis lose CK19 expression. Such phenomenon was observed in the case of some squamous cell carcinomas [25]. Two out of three H&E-positive but OSNA-negative NSCLC patients really had squamous cell carcinoma as a histological subtype.…”

Section: Comparison Of Hande and Osna Based On Relation To Prognosismentioning

confidence: 74%

Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure

Vodička

Pešta

Kulda

et al. 2020

Cells

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The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4–5 groups per patient) and examined by H&E, IHC CK19 and OSNA. The median follow-up was 44 months. Concordant results on the lymph node status of the H&E, IHC CK19 and OSNA examinations were reported in 78% of patients. We recorded shorter OS in patients with positive results provided by both OSNA and H&E. The study demonstrated a higher percentage of detected micrometastases in lymph nodes by the OSNA method. However, the higher sensitivity of the OSNA, with the cut-off value 250 copies of mRNA of CK19/µL, resulted in a lower association of OSNA positivity with progress of the disease compared to H&E. Increasing the cut-off to 615 copies resulted in an increase in concordance between the OSNA and H&E, which means that the higher cut-off is more relevant in the case of lung tumors.

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“…Consistency of CXCR4 expression within the tumour was analysed using the intraclass correlation coefficient (ICC), as earlier described 20. Associations between CXCR4 expression and tumour characteristics were tested by calculating Spearman rank correlation coefficient.…”

Section: Methodsmentioning

confidence: 99%

High CXCR4 expression in adenoid cystic carcinoma of the head and neck is associated with increased risk of locoregional recurrence

et al. 2020

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AimTreatment options for head and neck adenoid cystic carcinoma (AdCC) are limited in advanced disease. Chemokine receptor type 4 (CXCR4) is present in various tumour types, including AdCC. Upregulation is associated with tumour recurrence and metastasis. New CXCR4-specific diagnostic and therapeutic target agents have recently been available. This study aimed to analyse CXCR4 expression in a cohort of primary head and neck AdCC.MethodsAfter histopathological revision, tumour tissues of 73 consecutive patients with AdCC over 1990–2016 were sampled on a tissue microarray. Slides were immunohistochemically stained for CXCR4 and semiquantitatively scored. Associations between protein expression and cliniopathological parameters were tested. HRs were calculated using a Cox proportional hazard model.ResultsSixty-six tumours could be analysed. CXCR4 expression was present in 81% of the tumours with a median of 29% (IQR 1–70) positive cells. Expression was univariately correlated to perineural growth (Spearman ρ .26, p=0.04) and bone invasion (Spearman ρ .32, p=0.01), but not with tumour grade.CXCR4 expression in the primary tumour was significantly higher in tumours that recurred as compared with those that did not recur (median 60%, IQR 33–72 vs 12%, IQR 1–70, Kruskal-Wallis p=0.01). After dichotomisation, >25% of CXCR4 expressions proved an independent prognosticator for a reduced recurrence-free survival (RFS) (HR 7.2, 95% CI 1.5 to 72.4, p=0.04).ConclusionCXCR4 is expressed in the majority of primary AdCCs and independently correlated to worse RFS, suggesting CXCR4 as a target for imaging and therapy purposes in patients with advanced AdCC.

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Cytokeratin 19 expression in early oral squamous cell carcinoma and their metastasis: Inadequate biomarker for one-step nucleic acid amplification implementation in sentinel lymph node biopsy procedure

Cited by 14 publications

References 13 publications

One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma

One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma

Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure

High CXCR4 expression in adenoid cystic carcinoma of the head and neck is associated with increased risk of locoregional recurrence

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