Cytokeratin and Vimentin Co-Expression in 21 Canine Primary Pulmonary Epithelial Neoplasms

Abstract

Co-expression of cytokeratin and vimentin has been traditionally associated with a few select tumors. However, this phenomenon is being recognized in a wider range of tumors. Twenty-one canine primary pulmonary epithelial neoplasms were evaluated for the co-expression of cytokeratin and vimentin. The histologic pattern and grade, and an immunohistochemical grade for cytokeratin and vimentin staining, were determined for each neoplasm. Adenocarcinomas predominated, and histologically, most tumors were grade II. All of the neoplasms stained positive for cytokeratin, while only 8 (38%) stained positive for both vimentin and cytokeratin. Papillary adenocarcinomas were consistently vimentin negative. The anaplastic histologic pattern had significantly more vimentin staining than the other histologic patterns. There was no significant difference in histologic grade or grading criteria between those tumors that stained with vimentin and those that did not. The present study established that cytokeratin and vimentin co-expression occurs in canine primary pulmonary epithelial tumors at a similar frequency to human pulmonary neoplasms. Further investigation will be needed to characterize the significance of this finding, particularly with respect to prognosis.

Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs

“…Moreover, co-expression of cytokeratin and vimentin has also been found in tumors which, in cases of dissemination, can be included in differential diagnosis of visceral tumors, among them thyroid carcinomas, prostatic carcinomas, endometrial adenocarcinoma, ovarian epithelial tumors and mammary gland carcinomas (Grieco et al 2003, Riccardi et al 2007, Pires et al 2010. The study performed on the canine lung carcinomas has revealed co-expression of cytokeratin and vimentin in neoplastic cells in 38% of cases (Burgess and Kerr 2009). Additionally, cancers with more anaplastic appearance showed vimentin immunoreactivity more often than in moderate or well differentiated types of cancers (Burgess and Kerr 2009).…”

“…The study performed on the canine lung carcinomas has revealed co-expression of cytokeratin and vimentin in neoplastic cells in 38% of cases (Burgess and Kerr 2009). Additionally, cancers with more anaplastic appearance showed vimentin immunoreactivity more often than in moderate or well differentiated types of cancers (Burgess and Kerr 2009). Moreover, vimentin immunoreactivity in the prostatic carcinomas in both humans and dogs was considered to be associated with more invasive phenotype and thus typical for more disseminated forms of cancer (Grieco et al 2003, Ricardi et al 2007).…”

Respiratory Pathology and Pathogens in Wild Urban Rats (Rattus norvegicus and Rattus rattus)

“…Immunohistochemical (IHC) assays were performed on 1 lung tumor using murine monoclonal antibodies against cytokeratin (AE1/AE3; Sigma-Aldrich, Oakville, Canada) and vimentin (BioGenex, Fremont, CA) at 1:200 and 1:100 dilutions, respectively. 5 Samples or sterile swabs of all grossly observed purulent lesions underwent routine aerobic and anaerobic bacterial isolation and identification.…”

Resumos Do Xvi Enapave

“…As neoplasias pulmonares de origem epitelial ainda devem ser diferenciadas quanto à malignidade e tipo histológico (Wilson e Dungworth, 2002). O adenocarcinoma pulmonar do tipo acinar apresenta diversos padrões histológicos (Burgess e Kerr, 2009). Geralmente localiza-se no tecido pulmonar periférico e dissemina-se em vários outros nódulos (Wilson e Dungworth, 2002) ou ocorre na forma de massas sólidas, brancacentas e multifocais .…”