Cytokeratin Expression and Proliferative Activity of Keratocystic Odontogenic Tumor

Hayakawa, Masahide; Okada, Hiroyuki

doi:10.5466/ijoms.5.43

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…According to Stoll et al, the expression rates for CK13 in the lining epithelia were 100% in radicular cysts, 87% in DC, and 80% KCOT (11). Hayakawa et al reported that almost the entire spinous layer in KCOT was CK13 positive (13). The CK13 positivity may indicate their odontogenic origins as demonstrated also in ameloblastoma by Wato et al (18).…”

Section: Discussionmentioning

confidence: 96%

“…CK13 is acidic with a molecular weight of 54 kDa and is expressed in the spinous layer of the non-keratinized squamous epithelium (11,12). Specific CK subtype expression is associated with the development of odontogenic tumors and cysts and also aids in distinguishing these two cell types at the time of diagnosis (8,(13)(14)(15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

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Comparative expression profiles of keratins and apoptosis regulating proteins in keratocystic odontogenic tumor, orthokeratinized odontogenic cyst, and dentigerous cyst

et al. 2012

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Background: In addition to keratocystic odontogenic tumor (KCOT), orthokeratinized odontogenic cyst (OOC) has recently been recognized as a different jaw cyst entity whose lining epithelia are markedly keratinized. However, the odontogenic background of OOC remains still controversial. To clarify the difference in the lining epithelial natures of KCOT and OOC, we investigated differentiation modes for keratinization and neoplastic characteristics of these two cysts by immunohisto chemistry for keratin subtypes and apoptosisrelated proteins. Methods: Surgical specimens of KCOT (n = 20) and OOC (n = 15), as well as dentigerous cyst (DC, n = 15), which was used as a control, were examined by immunohistochemistry and western blotting for their expressions for cytokeratin (CK) 1, CK13, Bcl2, and Bax. Immunohistochemical reactions were evaluated separately in two epithelial layers: upper spinous and basal. Results: Most of the spinous cells in the upper layer of KCOT and DC were CK13 positive (90100%), while they were not as conspicuous in OOC (20%). In contrast, CK1 was positive in OOC (100%), while it was not positive in KCOT and DC. Basal layer cells in KCOT were positive for Bcl2 (100%) but not for Bax (0%). Instead, they were not positive for Bcl2 but positive Bax in OOC and DC. These immunohistochemical profiles in the three cystic lesions were confirmed by western blot analyses showing CK13 in KCOT and DC and CK1 in OOC. Conclusions: The CK expression modes were similar between KCOT and DC, indicating their odontogenic characteristics, which were not evident in OOC. The Bcl2 positivity in KCOT suggested its neoplastic nature, though there was no neoplastic evidence in OOC and DC.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Comparative expression profiles of keratins and apoptosis regulating proteins in keratocystic odontogenic tumor, orthokeratinized odontogenic cyst, and dentigerous cyst

et al. 2012

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The expression of cytokeratin in keratocystic odontogenic tumor, orthokeratinized odontogenic cyst, dentigerous cyst, radicular cyst and dermoid cyst

et al. 2013

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The epithelial lining of odontogenic keratocysts exhibits either parakeratosis or orthokeratosis. In 2005, the WHO classified odontogenic keratocysts with parakeratosis as keratocystic odontogenic tumors (KCOT). Odontogenic keratocysts with orthokeratosis were not classified as odontogenic tumors, but instead referred to as orthokeratinized odontogenic cysts (OOC). To clarify the difference between these two lesions, we investigated their biological characteristics using immunohistochemical studies for cytokeratins (CK) in KCOT and OOC as well as in dentigerous cysts (DC), radicular cysts (RC) and dermoid cysts (DMC). We examined twenty-five cases of KCOT, fifteen cases each of OOC, DC and RC, and ten cases of DMC. We studied the immunohistochemical expression of CK10, 13, 17 and 19. To evaluate the immunohistochemical staining pattern, we divided the epithelial lining of the lesions into three layers (surface layer: su, spinous layer: sp, basal layer: ba). For CK10, most OOC and DMC specimens of su and sp were positive. For CK13 and 19, most KCOT, DC and RC specimens of su and sp were positive. For CK17, most KCOT specimens of su and sp were positive. The percentages of total CK expression of su and sp, and ba of CK19 differed significantly between the lesions (P < 0.001). These results support the hypothesis that OOC originate from not the odontogenic apparatus, but the oral epithelial component.

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Expression of cytokeratin in keratocystic odontogenic tumors and orthokeratinized odontogenic cysts

Tsuji¹,

Wato²,

Matsuda³

et al. 2011

Jpn. J. Oral. Maxillofac. Surg.

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Cytokeratin Expression and Proliferative Activity of Keratocystic Odontogenic Tumor

Cited by 3 publications

References 22 publications

Comparative expression profiles of keratins and apoptosis regulating proteins in keratocystic odontogenic tumor, orthokeratinized odontogenic cyst, and dentigerous cyst

Comparative expression profiles of keratins and apoptosis regulating proteins in keratocystic odontogenic tumor, orthokeratinized odontogenic cyst, and dentigerous cyst

The expression of cytokeratin in keratocystic odontogenic tumor, orthokeratinized odontogenic cyst, dentigerous cyst, radicular cyst and dermoid cyst

Expression of cytokeratin in keratocystic odontogenic tumors and orthokeratinized odontogenic cysts

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