Cytokine expression in the gilt oviduct: Effects of seminal plasma, spermatozoa and extender after insemination

Jiwakanon, Jatesada; Berg, Mikael; Persson, Erik; Fossum, Caroline; Dalin, Anne-Marie

doi:10.1016/j.anireprosci.2010.01.005

Cited by 26 publications

(51 citation statements)

References 59 publications

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“…The authors suggested these increments could influence cytokine synthesis and leukocyte trafficking, and play a beneficial effect in regulating embryonic pre-implantation. Moreover, Jiwakanon et al (2010) found TGFβ1 mRNA expression to be significantly increased in the isthmus 40 hours after SP infusion compared to controls, and suggested these changes might benefit immune modulation, as previously suggested by Robertson (2005).…”

Section: Entry Of Semen Induces Gene Expression Changes In Female Gensupporting

confidence: 67%

“…sperm-peak fraction has already reached the tip of the uterine horns and probably a fraction of these spermatozoa already entered the sperm reservoir in the UTJ. Thus, it is logical to expect that the cytokines play differential roles in interacting with the local immune system in the female genitalia (Rozeboom et al, 2001;O'Leary et al, 2004;Jiwakanon et al, 2010;Rodriguez-Martinez et al, 2011). Even though few cytokines were reported previously in the boar SP (O'Leary et al, 2011;Jiwakanon et al, 2012), a full battery of cytokines in the SP of well-identified fractions of the boar ejaculate have been lacking until Paper I was published.…”

Section: Discussionmentioning

confidence: 99%

“…For this reason, many studies focused on the changes experienced by genes governing production of particular cytokines. For instance, polymerase chain reaction (PCR) studies revealed that cytokine expression in the female reproductive tract is modified by mating or artificial insemination (AI) in poultry (Das et al, 2006;Das et al, 2009) and pigs (O'Leary et al, 2004;Jiwakanon et al, 2010). For instance, AI of hens increased mRNA levels of interleukin-1β and lipopolysaccharide-induced TNF factor (LITAF) in vaginal tissues as early as 1-6 hours post insemination, while such changes were not evident in other segments of the oviduct (Das et al, 2009).…”

Section: Entry Of Semen Induces Gene Expression Changes In Female Genmentioning

confidence: 99%

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Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

Atikuzzaman¹

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Internal fertilization occurs in birds and eutherian mammals. Foetal development, however, is either extra-respectively intra-corpore (egg vs uterus). In these animal classes, the female genital tract stores ejaculated spermatozoa into a restricted oviductal segment; the functional pre-ovulatory sperm reservoir, where they survive until ovulation/s occur. Paradoxically, this immunologically foreign sperm suspension in seminal fluid/plasma, often microbiologically contaminated, ought to be promptly eliminated by the female local immune defence which, instead, tolerates its presence. The female immune tolerance is presumably signalled via a biochemical interplay of spermatozoa, as well as the peptides and proteins of the extracellular seminal fluid, with female epithelial and immune cells. Such interplay can result in gene expression shifts in the sperm reservoir in relation to variations in fertility. To further aid our understanding of the underlying mechanisms, this thesis studied the proteome of the seminal fluid (using 2D SDS-PAGE and mass spectrometry) including cytokine content (using Luminex and/or ELISA) of healthy, sexually mature and fertile boars and cocks. As well, gene expression changes (using cDNA microarray) in the oviductal sperm reservoirs of sexuallymature females, mated or artificially infused with homologous sperm-free seminal fluid/plasma were studied. Pigs were of commercial, fertility-selected modern breeds (Landrace), while chicken belonged to the ancestor Red Junglefowl (RJF, low egg laying-capacity), a selected egg-layer White Leghorn (WL) and of their Advanced Intercross Line (AIL). Ejaculates were manually collected as single sample in cocks or as the sperm-rich fraction [SRF] and the post-SRF fraction in boars to harvest seminal fluid/plasma for proteome/cytokine and infusionstudies. Oviducts were retrieved for gene-expression analyses via microarray immediately post-mortem (chicken) or at surgery (pig), 24 h after mating or genital infusion. In pigs, the protein-rich seminal plasma showed the highest amounts of cytokines [interferon-γ, interferon gamma-induced protein 10 (IP-10/CXCL10), macrophage derived chemokine (MDC/CCL22), growth-regulated oncogene (GRO/CXCL1), granulocyte-macrophage colony-stimulating factor (GM-CSF), monocyte chemo-attractant protein-1 (MCP-1/ CCL2), interleukin (IL)-6, IL-8/CXCL8, IL-10, IL-15, IL-17 and transforming growth factor (TGF)-β1-3) in the larger, protein-rich and sperm-poor post-SRF, indicating its main immune signalling influence. Chicken showed also a plethora of seminal fluid proteins with serum albumin and ovotransferrin being conserved through selection/evolution. However, they showed fewer cytokines than pigs, as the anti-inflammatory/immune-modulatory TGF-β2 or the proinflammatory CXCL10. The RJF contained fewer immune system process proteins and lacked TGF-β2 compared to WL and AIL, suggesting selection for increased fertility could be associated with higher expression of immune-regulating peptides/proteins. The oviductal sperm re...

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Section: Entry Of Semen Induces Gene Expression Changes In Female Gensupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Entry Of Semen Induces Gene Expression Changes In Female Genmentioning

confidence: 99%

See 1 more Smart Citation

Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

Atikuzzaman¹

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“…The presence of cytokines has been demonstrated in the mammalian oviduct (Fahey et al 2005, Jiwakanon et al 2010, an important site of gamete transport, fertilization, early embryo development and egg transport (Croxatto 1996). In contrast to other species (Hunt et al 1993, Srivastava et al 1996, Wijayagunawardane et al 2003, Reyes et al 2007), we did not find expression of TNF-a mRNA in the endosalpinx and myosalpinx of the rat oviduct, indicating that the regulation of the transcription of the TNF-a mRNA in the mammalian oviduct could be species specific.…”

Section: Discussionmentioning

confidence: 99%

Tumour necrosis factor-α is the signal induced by mating to shutdown a 2-methoxyestradiol nongenomic action necessary to accelerate oviductal egg transport in the rat

et al. 2013

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Mating shut down a 2-methoxyestradiol (2ME) nongenomic action necessary to accelerate egg transport in the rat oviduct. Herein, we investigated whether tumour necrosis factor-α (TNF-α) participates in this mating effect. In unmated and mated rats, we determined the concentration of TNF-α in the oviductal fluid and the level of the mRNA for Tnf-a (Tnf) and their receptors Tnfrsf1a and Tnfrsf1b in the oviduct tissues. The distribution of the TNFRSF1A and TNFRSF1B proteins in the oviduct of unmated and mated was also assessed. Finally, we examined whether 2ME accelerates oviductal egg transport in unmated rats that were previously treated with a rat recombinant TNF-α alone or concomitant with a selective inhibitor of the NF-κB activity. Mating increased TNF-α in the oviductal fluid, but Tnf transcript was not detected in the oviduct. The mRNA for TNF-α receptors as well as their distribution was not affected by mating, although they were mainly localized in the endosalpinx. Administration of TNF-α into the oviduct of unmated rats prevented the effect of 2ME on egg transport. However, the NF-κB activity inhibitor did not revert this effect of TNF-α. These results indicate that mating increased TNF-α in the oviductal fluid, although this not associated with changes in the expression and localization of TNF-α receptors in the oviductal cells. Furthermore, TNF-α mimicked the effect of mating on the 2ME-induced egg transport acceleration, independently of the activation of NF-κB in the oviduct. We concluded that TNF-α is the signal induced by mating to shut down a 2ME nongenomic action in the rat oviduct.

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“…We have previously studied the effects of SP on the cytokines TGF-β1, IL-6 and IL-10 in different parts of the oviduct [17] and endometrium [18] both shortly after insemination (5–6 h) and later (35–40 h). The results showed that at 35–40 h after infusion, not only SP but also semen extender (BTS) and spermatozoa in BTS, up-regulated TGF-β1 mRNA expression compared with the control (catheter insertion) in the oviduct (isthmus and infundibulum).…”

Section: Introductionmentioning

confidence: 99%

Seminal plasma did not influence the presence of transforming growth factor-β1, interleukine-10 and interleukin-6 in porcine follicles shortly after insemination

2013

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BackgroundThe effects of seminal plasma on the presence of the cytokines transforming growth factor (TGF)-β1, interleukin (IL)-10 and IL-6 in ovarian follicles and follicular fluid were studied shortly after insemination in gilts.Ovaries from gilts were sampled 5–6 h after insemination with either seminal plasma (SP), fresh semen in extender (Beltsville thawing solution, BTS), spermatozoa in extender (Spz), or only BTS (control).ResultsImmunohistochemical (IHC) labeling of TGF-β1, IL-10 and IL-6 was evident in the ovarian oocytes and granulosa cells independent of stage of follicular development (antral follicles). Theca interna cells were labeled to a high degree in mature follicles. No consistent differences between treatment groups could be observed for any of the cytokines.In follicular fluid, high concentrations of TGF-β1 were found while the levels of IL-10 and IL-6 were low. There were no differences between treatment groups.ConclusionsOur results show a presence of the cytokines TGF-β1, IL-6 and IL-10 in oocytes, granulosa and theca cells, as well as in the fluid of mature follicles suggesting a role of these cytokines in intra-ovarian cell communication. However, treatment (SP, fresh semen in BTS, spermatozoa in BTS or BTS) did not influence the IHC-labeling pattern or the levels of these cytokines in follicular fluid shortly after insemination.

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Cytokine expression in the gilt oviduct: Effects of seminal plasma, spermatozoa and extender after insemination

Cited by 26 publications

References 59 publications

Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

Seminal Influence of the Oviduct : Mating and/or semen components induce gene expression changes in the pre-ovulatory functional sperm reservoir in poultry and pigs

Tumour necrosis factor-α is the signal induced by mating to shutdown a 2-methoxyestradiol nongenomic action necessary to accelerate oviductal egg transport in the rat

Seminal plasma did not influence the presence of transforming growth factor-β1, interleukine-10 and interleukin-6 in porcine follicles shortly after insemination

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