Cytokine gene expression patterns associated with immunization against Marek's disease in chickens

Abdul-Careem, Mohamed Faizal; Hunter, Bruce; Parvizi, Payvand; Haghighi, Hamid R.; Thanthrige-Don, Niroshan; Sharif, Shayan

doi:10.1016/j.vaccine.2006.08.006

Cited by 86 publications

(49 citation statements)

References 44 publications

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“…Transcriptional activity of IL-6 and IL-18 were enhanced in our study and this results were in consistent with earlier study (Abdul-Careem et al, 2007;Heidari et al, 2008) as this cytokines were detected in MD susceptible birds and not in MD resistant ones. This results in our opinion speculated that, this cytokines may play important role in driving immune response to MDV infection to form lymphoma in susceptible lines.…”

Section: Discussionsupporting

confidence: 93%

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Th1 and Th2 Cytokines Activity during Transformation and Lymphoma Formation Stage in Chicken Naturally Infected with Marek's disease virus.

Abd-Ellatieff¹,

Rawash²

2017

IOSR JAVS

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Marek's disease virus (MDV), a highly transmissible cell-associated neuropathic oncogenic alphaherpes virus affecting poultry health, resulting in considerable economic losses in poultry industry The present study aimed to examine the transcriptional profiling of a panel of cytokines genes in the splenic tissues of special broiler Japanese chickens (70-80 days old) contracted natural infection with MDV despite of intensive care and vaccination policy adopted by HVT and CVI988/Rispene. SYBR Green-based, real-time (RT)-PCR protocol was used to quantitate cytokine mRNA in freshly collected spleen tissue of MDV infected and control chicken. Changes in the levels of spleen interleukins (IL) as IL-6, IL-10, IL-18 and IL-12P35, IL-4, interferon-gamma (IFN-γ) and inducible nitric oxide synthase (iNOS) mRNA was determined. Relative Messenger RNA (mRNA) expression levels of the above mentioned genes, and β-actin as a reference gene, were achieved. The results of quantitative real-time PCR (qPCR) assays using revealed significant up regulation in the expression levels of

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Section: Discussionsupporting

confidence: 93%

“…Significant lower expression of these two cytokines was observed in vaccinated protected group compared to unvaccinated controls. It was hypothesized that, lower expression of these two cytokines may be associated with protection conferred by vaccines (Abdul-Careem et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Th1 and Th2 Cytokines Activity during Transformation and Lymphoma Formation Stage in Chicken Naturally Infected with Marek's disease virus.

Abd-Ellatieff¹,

Rawash²

2017

IOSR JAVS

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“…Liver, cecal tonsil, spleen, and bursa of Fabricius samples were collected and sectioned into two portions: one was placed in a sample bag and stored at Ϫ80°C for viral genome copy number determination, as described previously (24), and the other was collected in a 1.5-ml sterile Eppendorf tube containing RNAlater (Invitrogen Canada, Inc., Burlington, Ontario, Canada) and stored at Ϫ80°C. The expression levels of the IFN-␣, IFN-␥, IL-10, and IL-12 p40 cytokine genes were evaluated by real-time quantitative PCR (RT-qPCR), with ␤-actin as a reference gene, as described previously (25)(26)(27).…”

Section: Methodsmentioning

confidence: 99%

Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

Deng

Sharif

Nagy

2013

Clin Vaccine Immunol

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Fowl adenoviruses (FAdVs) are a potential alternative to human adenovirus-based vaccine vectors. Our previous studies demonstrated that a 2.4-kb region at the left end of the FAdV-9 genome is nonessential for virus replication and is suitable for the insertion or replacement of transgenes. Our in vivo study showed that the virus FAdV-9⌬4, lacking six open reading frames (ORFs) at the left end of its genome, replicates less efficiently than wild-type FAdV-9 (wtFAdV-9) in chickens that were infected intramuscularly. However, the fecal-oral route is the natural route of FAdV infection, and the oral administration of a vaccine confers some advantages compared to administration through other routes, especially when developing an adenovirus as a vaccine vector. Therefore, we sought to investigate the effects of FAdV-9 in orally inoculated chickens. In the present study, we orally inoculated specific-pathogen-free (SPF) chickens with FAdV-9 and FAdV-9⌬4 and assessed virus shedding, antibody response, and viral genome copy number and cytokine gene expression in tissues. Our data showed that FAdV-9⌬4 replicated less efficiently than did wtFAdV-9, as evidenced by reduced virus shedding in feces, lower viral genome copy number in tissues, and lower antibody response, which are consistent with the results of the intramuscular route of immunization. Furthermore, we found that both wtFAdV-9 and FAdV-9⌬4 upregulated the mRNA expression of alpha interferon (IFN-␣), IFN-␥, and interleukin-12 (IL-12). In addition, there was a trend toward downregulation of IL-10 gene expression caused by both viruses. These findings indicate that one or more of the six deleted ORFs contribute to modulating the host response against virus infection as well as virus replication in vivo.

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“…cDNA was synthesized using random primers and a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). The primer pairs used for analysis of five specific genes (Table I) were designed with the PerlPrimer software according to Qiagen product instruction (http://www.qiagen.com), with b-actin as the reference gene (28,29 corresponding to 1 mg reverse-transcribed RNA, and 250 nM (optimized concentration) forward and reverse primers. Samples from three chickens were analyzed for each group.…”

Section: Real-time Pcrmentioning

confidence: 99%

Transgenic Eimeria tenella Expressing Enhanced Yellow Fluorescent Protein Targeted to Different Cellular Compartments Stimulated Dichotomic Immune Responses in Chickens

Huang

Zou

et al. 2011

The Journal of Immunology

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Eimeria tenella, one of the seven species of chicken coccidia, elicits protective immunity against challenge infection with both homologous and heterologous strains. We endeavor to use recombinant E. tenella as a vaccine vehicle for expressing and delivering pathogen Ags and investigate immune responses against these foreign Ags. In this study, two lines of transgenic E. tenella expressing a model Ag, enhanced yellow fluorescent protein (EYFP), targeted to the micronemes and to the cytoplasm of the recombinant parasites were constructed to study the impact of Ag compartmentalization on immunogenicity. The MTT assay, intracellular cytokine staining, and real-time PCR were performed to detect the EYFP-specific proliferation and effector functions of splenic lymphocytes of immunized chickens. ELISA was used to measure anti-EYFP IgG and IgA responses. The results showed that both lines of transgenic parasites stimulated EYFP-specific lymphocyte proliferation and IFN-γ expression in CD4 and CD8 T cells, whereas a higher level of Ag-specific lymphocyte proliferation was elicited by the transgenic line expressing microneme-targeted EYFP. Furthermore, this line stimulated stronger IgA response than the one expressing cytoplasm-targeted EYFP after the second immunization. Our findings are encouraging for further investigation of the effect of Ag compartmentalization in transgenic Eimeria on immunogenicity and for the development of a eukaryotic vaccine vector using genetically modified Apicomplexa parasites.

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Cytokine gene expression patterns associated with immunization against Marek's disease in chickens

Cited by 86 publications

References 44 publications

Th1 and Th2 Cytokines Activity during Transformation and Lymphoma Formation Stage in Chicken Naturally Infected with Marek's disease virus.

Th1 and Th2 Cytokines Activity during Transformation and Lymphoma Formation Stage in Chicken Naturally Infected with Marek's disease virus.

Oral Inoculation of Chickens with a Candidate Fowl Adenovirus 9 Vector

Transgenic Eimeria tenella Expressing Enhanced Yellow Fluorescent Protein Targeted to Different Cellular Compartments Stimulated Dichotomic Immune Responses in Chickens

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