Cytokine mucosal expression in ulcerative colitis, the relationship between cytokine release and disease activity

Pearl, Daniel S.; Shah, K. K.; Whittaker, Mark; Nitch-Smith, Harriet; Brown, James F.; Shute, Janis K.; Trebble, T M

doi:10.1016/j.crohns.2012.07.022

Cited by 40 publications

(21 citation statements)

References 38 publications

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“…Cytokine IL-8 is a neutrophil chemoattractant secreted by intestinal epithelial cells as a response to inflammatory stimuli, such as bacterial molecules (Lammers et al, 1994;Mitsuyama et al, 1994). Colonic IL-8 levels have been shown to correlate with the degree of intestinal inflammation and the number of neutrophils in the mucosa in UC and CD patients (Mitsuyama et al, 1994;Pearl et al, 2013). Undifferentiated HT-29 cells were used in the IL-8 assays due to the downregulation of IL-8 in LPS-induced differentiated HT-29 cells as well as due to the assay optimization in our previous studies using undifferentiated HT-29 cells (Böcker et al, 2000;Hiippala et al, 2016Hiippala et al, , 2020.…”

Section: Discussionmentioning

confidence: 99%

Novel Odoribacter splanchnicus Strain and Its Outer Membrane Vesicles Exert Immunoregulatory Effects in vitro

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Novel Odoribacter splanchnicus Strain and Its Outer Membrane Vesicles Exert Immunoregulatory Effects in vitro

et al. 2020

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“…Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) which is usually confined to the colon and rectum, with diffuse and superficial lesions of the mucosa [1]. Mucosal inflammation correlated with disease severity is UC characterised [2]. Rich evidences suggested that cytokines played an essential role in the mucosal inflammatory by promoting leukocyte migration to sites of inflammation and ultimately leading to tissue damage and destruction [3].…”

Section: Introductionmentioning

confidence: 99%

The Possible Anti‐Inflammatory Effect of Dehydrocostus Lactone on DSS‐Induced Colitis in Mice

Zhou

Zhang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Dehydrocostus lactone (DL), one of the main active constituents in Aucklandia lappa Decne. (Muxiang), reported to have anti-inflammatory, antiulcer, and immunomodulatory properties. However, the effect of DL on ulcerative colitis (UC) has not been reported. To analyze the anti-inflammatory potential role of DL in UC, we provide a mechanism for the pharmacological action of DL. Methods. The experimental model of UC was induced by using oral administration of 2% dextran sulfate sodium (DSS) with drinking water in BALB/c mice. Mesalazine (Mes, 0.52 g/kg/d), DL-high doses (DL-H, 20 mg/kg/d), DL-middle doses (DL-M, 15 mg/kg/d), DL-low doses (DL-L, 10 mg/kg/d) were gavaged once a day from day 4 to day 17. Disease activity index (DAI) was calculated daily. On day 18, mice were rapidly dissected and the colorectal tissues were used to detect the levels of UC-related inflammatory cytokines (TNF-α, IL-1β, MCP-1, MPO, SOD, IL-6, IL-17, and IL-23), IL-6/STAT3 inflammatory signaling pathway (iNOS, COX2, IL-6, GP130, L-17, and IL-23), and colorectal mucosal barrier-related regulatory factors (MUC2, XBP1s, and α-defensins) by ELISA or qRT-PCR. Results. DL reduced the colorectal inflammation histological assessment, decreased UC-related inflammatory cytokines (TNF-α, IL-1β, MCP-1, MPO, SOD, IL-6, IL-17, and IL-23), downregulated IL-6/STAT3 inflammatory signaling pathway (iNOS, COX2, IL-6, GP130, L-17, and IL-23), repaired the key colorectal mucosal barrier protein-MUC2, and inhibited the downstream pathway (XBP1s and α-defensin). Conclusions. DL possessed the potential of anti-inflammatory effect to treated colitis. The protective mechanism of DL may involve in reducing inflammation and improving colorectal barrier function via downregulating the IL-6/STAT3 signaling.

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“…IL-8 expression levels are high in the inflamed mucosa of patients with UC (17-19) and Pearl et al (12) reported that there was an association between the severity of inflammation and the mucosal concentration of IL-8. The present study showed that IL-8 expression levels were upregulated in the inflamed regions of the colon in patients with UC, and this effect was independent of patient background factors such as sex, location of lesions, type of disease, combinations of treatment, and presence/absence of recurrence.…”

Section: Discussionmentioning

confidence: 99%

“…As mentioned above, there are several reports on the role of MUC2 and LYPD8 in UC; however, only two studies have examined the role of MUC2 and LYPD8 in the context of severity of inflammation and gene expression in UC (12,13). Furthermore, to the best of our knowledge, there are no studies comparing their gene expression in the lesioned and non-lesioned regions of the colon in patients with UC.…”

Section: Introductionmentioning

confidence: 98%

IL-8 and LYPD8 expression levels are associated with the inflammatory response in the colon of patients with ulcerative colitis

et al. 2020

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Ulcerative colitis (UC) is an idiopathic chronic inflammatory disorder affecting the large intestine, which may involve mucosal degeneration. Glycoproteins, mucin2 (MUC2) and the LY6/PLAUR domain containing 8 (LYPD8) are present on the mucous layer of the colon and can hinder the invasion of bacteria, thus contributing to the prevention of colitis. The present study investigated the expression levels of interleukin-8 (IL-8), MUC2 and LYPD8 on the mucous membranes of patients with UC. A total of 18 patients with UC (6 females and 12 males) were examined. Biopsies of the lesions as well as matching normal membranes were obtained and the mRNA expression levels of IL-8, MUC2 and LYPD8 were compared. LYPD8 expression was downregulated in the lesions and the relapsing-remitting subtype of lesions was associated with higher levels of MUC2 expression compared with single attack and chronic lesions subtypes. A positive correlation between Matts' histopathological grade and IL-8, as well as a negative correlation between Matts' histopathological grade and LYPD8 were observed. The expression levels of LYPD8 were lower in highly active lesions and these levels decreased according to the intensity of the mucosal inflammation. Conversely, an increase in MUC2 expression levels may reflect the recovery of the outer mucus layer in the remission phase. Therefore, the examination of MUC2 and LYPD8 expression levels may be useful indicators of mucosal healing in patients with UC.Abbreviations: UC, ulcerative colitis; MUC2, mucin2; LYPD8, LY6/PLAUR domain containing 8; IL-8, interleukin-8

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Cytokine mucosal expression in ulcerative colitis, the relationship between cytokine release and disease activity

Cited by 40 publications

References 38 publications

Novel Odoribacter splanchnicus Strain and Its Outer Membrane Vesicles Exert Immunoregulatory Effects in vitro

Novel Odoribacter splanchnicus Strain and Its Outer Membrane Vesicles Exert Immunoregulatory Effects in vitro

The Possible Anti‐Inflammatory Effect of Dehydrocostus Lactone on DSS‐Induced Colitis in Mice

IL-8 and LYPD8 expression levels are associated with the inflammatory response in the colon of patients with ulcerative colitis

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