Cytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07

Štofilová, Jana; Langerholc, Tomaž; Botta, Cristian; Treven, Primož; Gradišnik, Lidija; Salaj, Rastislav; Šoltésová, Alena; Bertková, Izabela; Hertelyová, Zdenka; Bomba, Alojz

doi:10.1016/j.biopha.2017.07.138

Cited by 32 publications

(21 citation statements)

References 41 publications

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“…The beneficial effects of probiotics in the GI tract have been demonstrated for various disorders [19,27,42,43]. In this study, we investigated the effects of Lactobacillus reuteri DSM 17938 supplementation on the protection of gastric damage induced by 50% ethanol in mice.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus reuteri DSM 17938 Protects against Gastric Damage Induced by Ethanol Administration in Mice: Role of TRPV1/Substance P Axis

et al. 2019

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This study aimed to evaluate the effect of Lactobacillus reuteri DSM 17938 (DSM) on ethanol-induced gastric injury, and if its possible mechanism of action is related to inhibiting the transient receptor potential vanilloid type 1 (TRPV1). We evaluated the effect of supplementing 108 CFU•g body wt−1•day−1 of DSM on ethanol-induced gastric injury. DSM significantly reduced the ulcer area (1.940 ± 1.121 mm2) with 3 days of pretreatment. The effects of DSM supplementation were reversed by Resiniferatoxin (RTX), TRPV1 agonist (3 nmol/kg p.o.). Substance P (SP) (1 μmol/L per 20 g) plus 50% ethanol resulted in hemorrhagic lesions, and DSM supplementation did not reverse the lesion area induced by administering SP. TRPV1 staining intensity was lower, SP, malondialdehyde (MDA) and nitrite levels were reduced, and restored normal levels of antioxidant parameters (glutathione and superoxide dismutase) in the gastric mucosa in mice treated with DSM. In conclusion, DSM exhibited gastroprotective activity through decreased expression of TRPV1 receptor and decreasing SP levels, with a consequent reduction of oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus reuteri DSM 17938 Protects against Gastric Damage Induced by Ethanol Administration in Mice: Role of TRPV1/Substance P Axis

et al. 2019

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“…Furthermore, such anti-inflammatory bacteria also upregulate heat shock protein expression on colon IEC [ 48 ]. Recent data suggest that anti-inflammatory action of probiotics in the gut may also be mediated by IL-10 secreted by monocytes and macrophages [ 49 ].…”

Section: Iec and Immunitymentioning

confidence: 99%

Bacteriophages targeting intestinal epithelial cells: a potential novel form of immunotherapy

Górski

Jończyk‐Matysiak

Łusiak-Szelachowska

et al. 2017

Cell. Mol. Life Sci.

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In addition to their established role as a physical barrier to invading pathogens and other harmful agents, intestinal epithelial cells (IEC) are actively involved in local immune reactions. In the past years, evidence has accumulated suggesting the role of IEC in the immunopathology of intestinal inflammatory disorders (IBD). Recent advances in research on bacteriophages strongly suggest that—in addition to their established antibacterial activity—they have immunomodulating properties that are potentially useful in the clinic. We suggest that these immunomodulating phage activities targeting IEC may open novel treatment perspectives in disorders of the alimentary tract, particularly IBD.

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“…In addition, TLR9 signaling activated by immunostimulatory bacterial CpG DNA sequences is essential for mediating the anti-inflammatory effects of probiotic strains. IL-10 is an antiinflammatory cytokine that can promote B lymphocyte activation, produce a large number of antibodies, and promote humoral immune responses (Bae et al, 2016;Štofilová et al, 2017). IFN-α and IFN-γ can activate macrophages, enhance phagocytosis of pathogenic bacteria, and play an important role in anti-tumor immune regulation (Thakur et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Two strains of probiotic Lactobacillus enhance immune response and promote naive T cell polarization to Th1

Ren

Wang

Liu

et al. 2019

Food and Agricultural Immunology

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We co-cultured Caco-2 cells with Lactobacillus salivarius and L. plantarum in vitro to investigate their immunoregulatory mechanisms and detected Lactobacillus strains adhering to Caco-2 cells expressing mRNAs of cytokines and Toll-like receptors (TLRs) by fluorescent quantitative reverse transcription-polymerase chain reaction. The strains were intragastrically administered to mice. Thymus/spleen indexes were measured on different days. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, flow cytometry, and enzyme-linked immunosorbent assay were performed to assess different variables. Results showed increased mRNA expressions of T helper 1 (Th1), various cytokines, surface receptors, TLRs (2, 4, and 9), thymus/spleen indexes, spleen lymphocyte transformation rate, macrophage energy metabolism and phagocytosis, CD11c + CD80 + cell number, interferon-γ secretion, and sIgA secretion levels; inhibition of nuclear factor-κB inflammation signal pathway; and downregulated mRNA expression of specific interleukins. Both Lactobacillus strains can promote naive T cell differentiation (Th1) and participate in immunomodulatory responses, featuring a potential in prevention and management approaches for foodborne diseases. ARTICLE HISTORY

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Cytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07

Cited by 32 publications

References 41 publications

Lactobacillus reuteri DSM 17938 Protects against Gastric Damage Induced by Ethanol Administration in Mice: Role of TRPV1/Substance P Axis

Lactobacillus reuteri DSM 17938 Protects against Gastric Damage Induced by Ethanol Administration in Mice: Role of TRPV1/Substance P Axis

Bacteriophages targeting intestinal epithelial cells: a potential novel form of immunotherapy

Two strains of probiotic Lactobacillus enhance immune response and promote naive T cell polarization to Th1

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