Cytokinetic analysis of human leukemic cells in vivo with bromodeoxyuridine using air-dried smear.

A technique is described for rapid detection of S-phase cells of tumor tissues in smear specimens using bromodeoxyuridine (BrdU) immunostaining. Mouse NR-S1 tumors and human tumor specimens were prepared for smear cytology after incubation in RPMI 1640 culture medium containing 200 microM BrdU at 37 degrees C under 3 atm for 1 hr. Samples were fixed in 70% ethanol for 30 min and used immediately or air dried for 30 min. Samples were then denatured in either 4 N HCl or 0.07 N NaOH to prepare partially single-stranded DNA. Fixation with air drying for 30 min followed by 30 min in 70% ethanol and 1 min denaturation with 0.07 N NaOH resulted in satisfactory staining quality. Cultured tumor specimens were processed for routine paraffin sections after smears were made for cytology. The labeling indices of the smear specimens and of the paraffin sections gave similar results. This technique should be useful in evaluating the cell proliferative potential of tumor tissue in smear cytology without processing paraffin sections.

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Detection of S-Phase Cells in Smear Cytology Using in Vitro Bromodeoxyuridine Labeling

Sekine

Sano

Uehara

et al. 1996

Biotechnic & Histochemistry

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Cytokinetic analysis of human leukemic cells in vivo with bromodeoxyuridine using air-dried smear.

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Detection of S-Phase Cells in Smear Cytology Using in Vitro Bromodeoxyuridine Labeling

Detection of S-Phase Cells in Smear Cytology Using in Vitro Bromodeoxyuridine Labeling

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