Cytokinin activity during early kernel development corresponds positively with yield potential and later stage ABA accumulation in field-grown wheat (Triticum aestivum L.)

Nguyen, Hai Ngoc; Perry, Laura E.; Kisiała, Anna; Olechowski, Henry; Emery, R. J. Neil

doi:10.1007/s00425-020-03483-2

Cited by 21 publications

(42 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At any stage of development, there is expression of a CGT, with the most limited expression being in grain at soft dough. As a group, the cCOGTs show little expression in the developing grain which is in general agreement with the relatively low expression detected in the RT-qPCR analyses from both Song et al (2012) and Nguyen et al (2020). However, expression of CNOGT3A, B and D, CONGT3A010300, and several COGTs is evident in the developing grain.…”

Section: Expression Of Cgts In Wheatsupporting

confidence: 83%

“…Generally, the expression of cZOGT1, cZOGT2-1 and cZOGT2-2 was elevated across the three stages examined with cZOGT2-2 being the most strongly expressed and particularly elevated at 1-2 DAA. Importantly, Nguyen et al (2020) note that the levels were variable among their five wheat cultivars, with the higher yielding cultivars having lesser expression of the cZOGTs.…”

Section: Expression Of Cgts In Wheatmentioning

confidence: 96%

“…TraesCS6A02G059300.1 TaCNGT6A059300.1 TacZOG2-2 (2B) showed strong expression across a range of cultivars immediately post-anthesis (Nguyen et al, 2020;Song et al, 2012). As this wheat GFM is closely related to rice cZOG1, which had increased yield when expression was decreased by RNAi, it may be a useful GFM to target via TILLING or gene editing.…”

Section: Expression Of Cgts In Wheatmentioning

confidence: 99%

See 2 more Smart Citations

Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement

Chen

Zhao

Song

et al. 2021

Plant Biotechnology Journal

View full text Add to dashboard Cite

The cytokinins, which are N 6 -substituted adenine derivatives, control key aspects of crop productivity. Cytokinin levels are controlled via biosynthesis by isopentenyl transferase (IPT), destruction by cytokinin oxidase/dehydrogenase (CKX), and inactivation via glucosylation by cytokinin glucosyl transferases (CGTs). While both yield components and tolerance to drought and related abiotic stressors have been positively addressed via manipulation of IPT and/or CKX expression, much less attention has been paid to the CGTs. As naming of the CGTs has been unclear, we suggest COGT, CNGT, CONGT and CNOGT to describe the O-, N-and dual function CGTs. As specific CGT mutants of both rice and arabidopsis showed impacts on yield components, we interrogated the wheat genome database, IWGSC RefSeq v1.0 & v2.0, to investigate wheat CGTs. Besides providing unambiguous names for the 53 wheat CGTs, we show their expression patterns in 70 developmental tissues and their response characteristics to various stress conditions by reviewing more than 1000 RNA-seq data sets. These revealed various patterns of responses and showed expression generally being more limited in reproductive tissues than in vegetative tissues. Multiple cis-regulatory elements are present in the 3 kb upstream of the start codons of the 53 CGTs. Elements associated with abscisic acid, light and methyl jasmonate are particularly over-represented, indicative of the responsiveness of CGTs to the environment. These data sets indicate that CGTs have potential value for wheat improvement and that these could be targeted in TILLING or gene editing wheat breeding programmes.genes controlling the formation of the deactivated cytokinin Oand N-glucosides. After introducing the cytokinins more generally, this article focuses on the cytokinin O-and N-glucosyl transferases and their potential roles in modifying yield in bread wheat, under ambient and stress conditions. Cytokinin biosynthesis and metabolismNaturally occurring cytokinins, which are adenine derivatives, fall into two groups: those with an isoprenoid side chain and those with an aromatic one. The isoprenoid cytokinins are considered to be the predominant forms (Jaworek et al., 2019;Sakakibara, 2006). The aromatics are not covered in this review as, while not exhaustively looked for in all cereals, they were not detected in either maize leaves (Lacuesta et al., 2018) or in rice (Kudo et al., 2012), although low levels of 6-benzylamino purine were recently reported in wheat spikes (Jabło nski et al., 2020) and low levels of various aromatic metabolites have been detected in germinating seeds of maize and oats (Stirk et al., 2012).

show abstract

Section: Expression Of Cgts In Wheatsupporting

confidence: 83%

Section: Expression Of Cgts In Wheatmentioning

confidence: 96%

Section: Expression Of Cgts In Wheatmentioning

confidence: 99%

See 1 more Smart Citation

Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement

Chen

Zhao

Song

et al. 2021

Plant Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…TaCKX1, TaCKX2.2.1 and TaCKX2.2.2 are mainly expressed in developing spikes, TaCKX3 and TaCKX10 in seedling roots, TaCKX5, TaCKX9 and TaCKX8 in younger organs of developing plants from seedling roots to 0 days after pollination (DAP) spikes, and TaCKX11 and TaCKX4 in all organs [32,33]. Similar tissue specificity of expression of TaCKX GFMs was presented based on RNA-seq data [12], as well as for TaCKX1 and TaCKX2 during early kernel development [13].…”

Section: Introductionsupporting

confidence: 61%

“…The content of ABA in seven DAP spikes, which is the milk/early dough stage of wild-type wheat plants was low, about 2.6 ng/g biomass [11] and decreased by 50% in TaCKX2.2.2 silenced plants. Since the highest concentrations of ABA were detected in the late dough stage [13], we may suppose that the lower level of ABA in seven DAP spikes resulted in extending chlorophyll content in flag leaves and also the maturation time of the spikes, which led to higher TGW. High content of cZ was observed during early embryo development of barley, underlying its important role in this stage of development [57].…”

Section: Regulation Of Developmental Processes and Yield-related Traitsmentioning

confidence: 99%

TaCKX2.2 Genes Coordinate Expression of Other TaCKX Family Members, Regulate Phytohormone Content and Yield-Related Traits of Wheat

Jabłoński

Szala

Przyborowski

et al. 2021

IJMS

View full text Add to dashboard Cite

TaCKX gene family members (GFMs) play essential roles in the regulation of cytokinin during wheat development and significantly influence yield-related traits. However, detailed function of most of them is not known. To characterize the role of TaCKX2.2 genes we silenced all homoeologous copies of both TaCKX2.2.1 and TaCKX2.2.2 by RNAi technology and observed the effect of silencing in 7 DAP spikes of T1 and T2 generations. The levels of gene silencing of these developmentally regulated genes were different in both generations, which variously determined particular phenotypes. High silencing of TaCKX2.2.2 in T2 was accompanied by slight down-regulation of TaCKX2.2.1 and strong up-regulation of TaCKX5 and TaCKX11, and expression of TaCKX1, TaCKX2.1, and TaCKX9 was comparable to the non-silenced control. Co-ordinated expression of TaCKX2.2.2 with other TaCKX GFMs influenced phytohormonal homeostasis. Contents of isoprenoid, active cytokinins, their conjugates, and auxin in seven DAP spikes of silenced T2 plants increased from 1.27 to 2.51 times. However, benzyladenine (BA) and abscisic acid (ABA) contents were significantly reduced and GA3 was not detected. We documented a significant role of TaCKX2.2.2 in the regulation of thousand grain weight (TGW), grain number, and chlorophyll content, and demonstrated the formation of a homeostatic feedback loop between the transcription of tested genes and phytohormones. We also discuss the mechanism of regulation of yield-related traits.

show abstract

Cytokinin seed priming mediated induction of terminal heat stress tolerance and expression profiling of SKP1 transcripts, a component of ubiquitin proteasome system in bread wheat (Triticum aestivum L.): a transgenerational analysis

Jaiswal

Sahi²,

Barthakur³

2022

Plant Growth Regul

View full text Add to dashboard Cite

Cytokinin activity during early kernel development corresponds positively with yield potential and later stage ABA accumulation in field-grown wheat (Triticum aestivum L.)

Cited by 21 publications

References 75 publications

Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement

Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement

TaCKX2.2 Genes Coordinate Expression of Other TaCKX Family Members, Regulate Phytohormone Content and Yield-Related Traits of Wheat

Cytokinin seed priming mediated induction of terminal heat stress tolerance and expression profiling of SKP1 transcripts, a component of ubiquitin proteasome system in bread wheat (Triticum aestivum L.): a transgenerational analysis

Contact Info

Product

Resources

About