Cytokinins and leaf development in sweet pepper (Capsicum annuum L.)

Ulvskov, Peter; Nielsen, Theodor; Seiden, Piet; Marcussen, Jan

doi:10.1007/bf01160714

Cited by 27 publications

(4 citation statements)

References 21 publications

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“…The treatments in which NAA was applied individually yielded better results. Similar observations were reported by Ulskov et al (1992). Our results have indicated that NAA has a positive effect on vegetative growth of spinach plants when applied at the rate of 10 µM both alone and in combination with BAP ( Figure 2).…”

Section: Discussionsupporting

confidence: 90%

Naphthalene Acetic Acid and Benzylaminopurine Enhance Growth and Improve Quality of Organic Spinach in Kitchen Gardens

2015

J. Bioresource Manage.

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The present study was conducted at the Kitchen Gardening Training Centre, Faisalabad, Pakistan to evaluate the effect of the plant growth regulators Naphthalene Acetic Acid (NAA) and Benzylaminopurine (BAP), on the growth and yield of organic spinach (Spinacia oleracea L.) in kitchen gardening. Different combinations and concentrations of NAA and BAP were tested to evaluate different vegetative parameters. The data were recorded at 40 and 60 days after sowing (DAS). Maximum plant height at 40 DAS was recorded for the combined effect of NAA and BAP at a concentration of 1000 µM (each), while BAP alone at 100 µM concentration showed maximum plant height at 60 DAS. Maximum number of leaves was shown by NAA (10 µM) both at 40 and 60 DAS. Increase in leaf length was observed for NAA (10 µM) and BAP (100 µM) both at 40 and 60 DAS. Significant increases in root length were recorded where maximum root length was in plots treated with BAP (1000 µM) applied 40 DAS while the mixture of BAP (10 µM) and NAA (10000 µM) induced significant increases in root length when applied 60 DAS. Maximum fresh weight of shoot was observed for NAA (1000 µM) and BAP (1000 µM) while maximum shoot dry weight was observed in plots treated with NAA and BAP at a concentration of (1000 µM) each. Similarly, a significant effect of plant growth regulators was observed on root dry weight where highest dry weight was noted in the plants treated with NAA at the rate of 1000 µM.

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Section: Discussionsupporting

confidence: 90%

Naphthalene Acetic Acid and Benzylaminopurine Enhance Growth and Improve Quality of Organic Spinach in Kitchen Gardens

2015

J. Bioresource Manage.

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“…After the columns were washed with water, the fraction containing the cytokinin bases and ribosides were eluted from the RP C 18 column with 10 ml of 80% methanol. The eluate was concentrated and applied to an immunoaffinity, prepared with monoclonal anti-ZR antibodies, which are able to bind a broad spectrum of cytokinins (Ulvskov et al 1992 ). After washing with 10 ml of water, the immunoaffinity column was eluted with 4 ml of ice-cold 100% methanol and immediately reconditioned with water; the eluate, containing the cytokinin free bases, ribosides and N 9 -glucosides, was dried and redissolved in 100 μl 100% methanol before storage at −70°C, until further analysis by ACQUITY TM Tandem Quadrupole Ultra Performance Liquid Chromatography-Mass spectrometry (ACQUITY TM TQD UPLC-MS/MS (Waters)).…”

Section: Methodsmentioning

confidence: 99%

Selection of plastid- and nuclear-encoded reference genes to study the effect of altered endogenous cytokinin content on photosynthesis genes in Nicotiana tabacum

et al. 2009

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Selection and use of appropriate reference genes as internal controls in real-time reverse transcription PCR (RT-PCR) assays is highly important for accurate quantification of gene expression levels. Since some photosynthetic genes are encoded in the nuclear genome and others in the chloroplast genome, we evaluated both nuclear-and plastid-encoded candidate reference genes. Six plastid-encoded candidate reference genes were derived from Arabidopsis microarray data and three plastid-and five nuclear-encoded reference genes were derived from literature. Cytokinins influence photosynthetic gene expression, so we evaluated the expression stability of the candidate reference genes in transgenic Nicotiana tabacum plants with elevated or diminished cytokinin content. We found that the most reliable strategy makes use of plastidencoded genes for normalizing plastid photosynthetic genes and nuclear-encoded reference genes for normalizing nuclear photosynthetic genes. Compared to the use of nuclear reference genes only, this approach assimilates any effects on transcriptional activity of chloroplasts or number of chloroplast. The best expression stabilities in Nicotiana tabacum were observed for the plastid-encoded references genes Nt-RPS3, Nt-NDHI and Nt-IN1 and for the nuclear-encoded genes Nt-ACT9, Nt-aTUB and Nt-SSU. These genes may be suitable for normalization of photosynthetic genes under other experimental conditions in Nicotiana tabacum, and orthologues of these genes may be suitable candidates for normalizing photosynthetic gene expression in other species.

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“…The eluate was dried in vacuo to remove the methanol and then applied to a combination of a cytokinin immunoaffinity and a RP-C18 column. The immunoaffinity columns were prepared with monoclonal ZR antibodies able to bind a broad spectrum of cytokinins [21]. After washing with 10 ml of water, the immunoaffinity column was eluted with 4 ml of ice-cold 100% methanol and immediately rinsed with water.…”

Section: Extraction and Purification Of Cytokinins Iaa And Abamentioning

confidence: 99%

Levels of endogenous cytokinins, indole‐3‐acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY‐2 cells

Redig¹,

et al. 1996

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Correlation between cell cycle progression and endogenous levels of plant hormones was studied in synchronized tobacco BY-2 cell suspension cultures. Sixteen different cytokinins, indole-3-acetic acid (IAA) and abscisic acid (ABA) were extracted using solid-phase anion exchange chromatography in combination with immunoaffinity purification, and quantified by mass spectrometry. No significant correlation could be identified for IAA and ABA. In contrast, there were sharp peaks in the levels of specific cytokinins (zeatin-and dihydrozeatin-type) at the end of the S phase and during mitosis. The levels of other cytokinins analyzed, including zeatins N-and O-glucosides, remained low, suggesting that the increased amounts of their corresponding non-glucosylated form resulted from de novo synthesis. These f'mdings suggest that zeatin-and dihydrozeatintype cytokinins might play a specific regulatory role in the progression of the plant cell cycle. One hypothesis to explain cytokinin action is based on a specific interaction with kinases that regulate cell cycle progression, as has been recently shown for the cytokinin analogue olomoucine.

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Cytokinins and leaf development in sweet pepper (Capsicum annuum L.)

Cited by 27 publications

References 21 publications

Naphthalene Acetic Acid and Benzylaminopurine Enhance Growth and Improve Quality of Organic Spinach in Kitchen Gardens

Naphthalene Acetic Acid and Benzylaminopurine Enhance Growth and Improve Quality of Organic Spinach in Kitchen Gardens

Selection of plastid- and nuclear-encoded reference genes to study the effect of altered endogenous cytokinin content on photosynthesis genes in Nicotiana tabacum

Levels of endogenous cytokinins, indole‐3‐acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY‐2 cells

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