Cytologic changes in the nasal secretions during the late nasal response

Dynamics of nasal eosinophils in response to a nonnatural allergen challenge in patients with allergic rhinitis and control subjects: A biopsy and brush study1

“…Studies performed until now were mainly focused on the acute 5,1~ and late phase reactions. [6][7][8]24 Only a few studies have been concerned with activated eosinophils in various mucosal tissues. 2~ Different results have been obtained by various investigators, depending on the cell sampling technique used.…”

Cellular changes in tears associated with keratoconjunctival responses induced by nasal allergy

“…These links allow the nasal allergic reaction to affect the conjunctiva in various ways upon the involvment of various mechanisms. [3][4][5][7][8][9][10][12][13][14][15][16][17][18][19][20][21][22][29][30][31]37 Diagnostic confirmation of hypersensitivity mechanism(s) underlying the allergic keratoconjunctivitis consists usually of (1) an ophthalmologic examination, evaluation of the cornea by slit-lamp vital staining with fluorescein, and cytologic examination of the conjunctiva using brush, impression, or scraping techniques; (2) determination of specific Keratoconjunctival response-cytologic changes in tears Z Pelikan…”

“…7,[29][30][31] Specimens were dehydrated by methyl alcohol, mounted in Canada balsam and scanned microscopically. 7,[29][30][31] The absolute cell numbers were counted per microscopic field at magnification  250 and mean values were calculated from 20 fields for each eye separately. The mean values from both the eyes were finally expressed in number of cells per microscopic field (  250 magnification), per one eye.…”

“…The first air-dried series was fixed by polyethylene glycol and stained by Hansel's method, the second air-dried series was stained by May-Grü nwald-Giemsa, modified by us, and the third methanol fixed series was stained by the toluidine blue method. 7,[29][30][31] Specimens were dehydrated by methyl alcohol, mounted in Canada balsam and scanned microscopically. 7,[29][30][31] The absolute cell numbers were counted per microscopic field at magnification  250 and mean values were calculated from 20 fields for each eye separately.…”

“…In these 61 patients, 94 NPTs with inhalant allergens (Supplementary Table 2S), with respect to the positive skin tests and/or suspect disease history, and 61 phosphate-buffered saline (PBS) control tests were performed by means of rhinomanometry [7][8][9][10]26,29,30 combined with the recording of ocular signs and symptoms. [7][8][9][10]26,27 The patients were investigated in a period without acute ocular and nasal complaints and outside the allergen-relevant season.…”