Cytological evaluation of the influence of high and low doses of bisphenol A on an erythroblastic cell line of porcine bone marrow

Snarska, Anna; Wysocka, Dominika; Rytel, Liliana; Makowska, Krystyna; Gonkowski, Sławomir

doi:10.2478/jvetres-2018-0068

Cited by 5 publications

(2 citation statements)

References 21 publications

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“…59 A harmful effect of BPA on haematopoietic processes was proved on an erythroblastic cell line of porcine bone marrow. 60 All these reports show a negative effect of BPA on an erythroid cell line that is in contrast with the results of our study, where a positive correlation of BPA concentration and erythroid cell line indices was found. The haemolytic effect of bisphenols has been reported in rats, together with an increase of bilirubin concentration.…”

Section: Discussioncontrasting

confidence: 99%

Serum concentration of bisphenol A in elderly cats and its association with clinicopathological findings

Kovaříková

Maršálek

Habánová

et al. 2020

Journal of Feline Medicine and Surgery

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Objectives Bisphenol A (BPA) has been mentioned as a possible factor contributing to feline hyperthyroidism. Nevertheless, there are no previous studies reporting on the concentration of BPA in feline serum and its association with thyroid function. The objectives of this study were to measure serum BPA concentration in cats aged ⩾7 years, considered as healthy by their owners, and to compare the results with clinicopathological findings. Methods Sixty-nine cats aged ⩾7 years considered as healthy by their owners were enrolled in the study. The concentration of BPA in feline serum was measured using liquid chromatography–tandem mass spectrometry. In all cats, signalment, living environment, diet history, and the results of haematological and biochemical analysis, including thyroxine levels, were available. Results The mean serum BPA concentration in feline serum was 1.06 ± 0.908 ng/ml. Significant correlation was found between BPA concentration and haemoglobin ( r = 0.3397; P = 0.0043), haematocrit ( r = 0.3245; P = 0.0065) and the number of red blood cells ( r = 0.2916; P = 0.0151), concentration of total protein ( r = 0.2383; P = 0.0486), concentration of calcium ( r = 0.3915; P = 0.0009) and level of bilirubin ( r = 0.3848; P = 0.0011). No other significant correlations were found. Significant differences ( P <0.01) were found between mature (1.28 ± 0.994 ng/ml) and geriatric cats (0.420 ± 0.240 ng/ml), between strictly indoor cats (1.27 ± 0.992 ng/ml) and cats with outdoor access (0.660 ± 0.529 ng/ml), and between cats fed canned food (1.23 ± 0.935 ng/ml) and cats fed non-canned food (0.774 ± 0.795 ng/ml). Conclusions and relevance Measurable serum BPA levels were found in all examined samples. The age of the cats was revealed as a significant factor affecting BPA concentration and mature cats had the highest levels. A significantly higher concentration of BPA was found in cats living strictly indoors and in cats fed canned food. No association was found between BPA and thyroid function. Further studies are needed that focus on hyperthyroid cats for better evaluation of this relationship.

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Section: Discussioncontrasting

confidence: 99%

Serum concentration of bisphenol A in elderly cats and its association with clinicopathological findings

Kovaříková

Maršálek

Habánová

et al. 2020

Journal of Feline Medicine and Surgery

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“…Even though these bodies are often present in erythrocytes of healthy animals, they occur in greater numbers of erythrocytes in animals that have been poisoned with substances commonly found in the environment, e.g . bisphenol A ( 13 ). Therefore, an important conclusion of this study is that changes in the number of erythroblasts and disturbances in ratios between them in groups receiving low and high doses of acrylamide in comparison to the control group disturb the process of erythropoiesis in porcine bone marrow.…”

Section: Discussionmentioning

confidence: 99%

The influence of high and low doses of acrylamide on porcine erythropoiesis

Snarska

Palus

Wysocka

et al. 2020

Journal of Veterinary Research

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IntroductionDue to the widespread occurrence of acrylamide in the environment, its likely carcinogen status, and the suitability of the pig model as a human analogue, the authors decided to evaluate the impact of high and low doses of this compound on the processes of erythropoiesis in swine bone marrow.Material and MethodsThe experiment was carried out on Danish Landrace pigs at the age of eight weeks and body weight about 20 kg. The animals were divided into three equal groups consisting of five pigs in each. Control animals received empty gelatin capsules (placebos). Animals from the first experimental group received a low dose of acrylamide of 0.5 μg/kg b.w./day (> 99% purity; Sigma-Aldrich, Poland), and animals from the second experimental group received a dose 10 times higher. Placebos and acrylamide capsules were administered with feed every morning for 28 days. After anaesthetisation of the animals, bone marrow from the femur was collected into tubes without an anticoagulant on days 0 and 28. After drying and staining, bone marrow smears were subjected to detailed cytological evaluation using a light microscope.ResultsThis study showed that high and low doses of acrylamide affected the process of porcine erythropoiesis. The cytotoxic effect of acrylamide on this process was demonstrated in a change of the polychromatic erythroblasts/normochromatic erythroblasts ratio.ConclusionBoth doses of acrylamide caused a decrease in the number of ortho- and polychromatic erythroblasts.

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Concentrations of bisphenol a (BPA) in fresh pork loin meat under standard stock-farming conditions and after oral exposure – A preliminary study.

et al. 2022

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Cytological evaluation of the influence of high and low doses of bisphenol A on an erythroblastic cell line of porcine bone marrow

Cited by 5 publications

References 21 publications

Serum concentration of bisphenol A in elderly cats and its association with clinicopathological findings

Serum concentration of bisphenol A in elderly cats and its association with clinicopathological findings

The influence of high and low doses of acrylamide on porcine erythropoiesis

Concentrations of bisphenol a (BPA) in fresh pork loin meat under standard stock-farming conditions and after oral exposure – A preliminary study.

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