Cytology of Amniotic Fluid

Wachtel, E; Gordon, H.; Olsen, E. G. J.

doi:10.1111/j.1471-0528.1969.tb06145.x

Cited by 30 publications

(16 citation statements)

References 3 publications

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“…Total and viable cells were counted using light microscopy, viable cells excluded the dye and remained golden yellow in colour whilst dead cells stained blue [3]. All cell counts were performed twice and the mean number accepted.…”

Section: Patients Methods and Resultsmentioning

confidence: 99%

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Why Cell Culture Is Successful after Early Amniocentesis

Byrne¹,

Azar²,

Nicolaides³

1991

Fetal Diagn Ther

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The current interest in early amniocentesis as a possible alternative to chorion villus sampling has driven many centres to attempt cell culture from first trimester amniotic fluid. This study examines the cellular content of 125 amniotic fluid specimens collected between 8 and 18 weeks of gestation and shows that there is a higher proportion of viable cells at the time of early amniocentesis than at traditional amniocentesis. Although there is a small increase in viable cell number from 8 to 18 weeks of gestation, this is not as dramatic as the exponential rise in total cell number seen over the same period. The similar concentration of viable cells in early pregnancy to that in the second trimester is sufficient to explain the unexpected culture success after early amniocentesis.

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Section: Patients Methods and Resultsmentioning

confidence: 99%

“…The major limitations to earlier amnio centesis were considered to be: access to the uterus, and an inadequate number of free cells in amniotic fluid to initiate a viable cul ture [3]. The former has been overcome with high resolution ultrasound for needle guid ance, but what of the latter?…”

Section: Introductionmentioning

confidence: 99%

Why Cell Culture Is Successful after Early Amniocentesis

Byrne¹,

Azar²,

Nicolaides³

1991

Fetal Diagn Ther

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“…This confirms our observation in earlier studies (Ferguson-Smith and Ferguson-Smith, 1970) that amniotic specimens obtained before 14 weeks and possibly after 30 weeks are less satisfactory than specimens obtained at 14 to 30 weeks. This is probably related to the low cell count in early pregnancy and the large number of anucleate cells in later pregnancy (Votta et al, 1968;Wachtel et al, 1969). 71 One culture (B29) is of special interest as all mitoses were tetraploid.…”

Section: Hysterotomy Seriesmentioning

confidence: 99%

Chromosome Analysis before Birth and its Value in Genetic Counselling

Ferguson-Smith¹,

Ferguson‐Smith²,

Nevin³

et al. 1971

BMJ

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“…It is now accepted that the cells present in amniotic fluid are of fetal origin (Votta et al, 1968;Wachtel et al, 1969). The exact source of these cells, however, has not yet been established (Wachtel et al, 1969), though it seems likely that skin and amnion are the major source (Votta et al, 1968).…”

Section: Introductionmentioning

confidence: 99%

“…The exact source of these cells, however, has not yet been established (Wachtel et al, 1969), though it seems likely that skin and amnion are the major source (Votta et al, 1968).…”

Section: Introductionmentioning

confidence: 99%

Amniotic Fluid Cells; Prenatal Sex Prediction and Culture

Nelson

Emery

1970

Obstetrical & Gynecological Survey

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, 1970, 1, 523-526 Summary: Sex chromatin studies were carried out on small amounts of amniotic fluid obtained by amniocentesis or from intact amniotic sacs removed at hysterotomy. Provided that satisfactory preparations were obtained the accuracy of fetal sexing was 87%. Nevertheless, in the management of a pregnancy in which there is a risk of a serious X-linked recessive disorder, repeat amniocentesis may be necessary to ensure satisfactory specimens.Of 90 samples of fluid cultured, satisfactory growth was obtained in 49; the success rate was not increased by the addition of stimulants to the culture medium. It is suggested that between the 13th and the 16th week of pregnancy is the optimum time for amniocentesis to obtain cells for culture, since sufficient cells are then present in a small volume of fluid and therapeutic abortion would still be possible once the results were available.

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Cytology of Amniotic Fluid

Cited by 30 publications

References 3 publications

Why Cell Culture Is Successful after Early Amniocentesis

Why Cell Culture Is Successful after Early Amniocentesis

Chromosome Analysis before Birth and its Value in Genetic Counselling

Amniotic Fluid Cells; Prenatal Sex Prediction and Culture

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