Cytometric Analysis of DNA Changes Induced by Sulfur Mustard

Protective effects of the thiol compounds GSH and NAC against sulfur mustard toxicity in a human keratinocyte cell line

“…However, 1000 mM SM induced a near 100% cytotoxicity. Doses of 30, 100 and 300 mM SM which induced toxic effects in a dose-dependent manner were thus chosen for further investigation, in line with previous data obtained by others (Smith et al, 1993). In general, toxic effects of SM exposure can be reduced by treatment with the thiol compounds GSH and NAC which is in agreement with the other studies using SM or the monofunctional alkylating compound CEES (Amir et al, 1998;Jugg et al, 2013;Paromov et al, 2011;Paromov et al, 2007;TewariSingh et al, 2011).…”

Acute effects of sulfur mustard injury—Munich experiences

“…The toxic mechanism of SM poisoning has been linked to DNA damage and resulting cell signalling Ruff and Dillman, 2007). As SM is mainly not directly cytotoxic the exposed cells halt in the cell cycle, initiate repair and either survive or execute apoptosis which needs several hours to be completed (Matijasevic and Volkert, 2007;Rosenthal et al, 1998;Smith et al, 1993). Even the release of proinflammatory cytokines is detectable only after several hours (Arroyo et al, 2000).…”

Comparison of cell size in sulfur mustard-induced death of keratinocytes and lymphocytes†

“…24 Sulfur mustard does inhibit NHEK from undergoing mitosis; it has been shown that HD blocks NHEK cell cycle progression. 7,8,25 The NHEK enlargement would account for the decrease in NHEK recovery seen even at HD concentrations as low as 0.01 mM, 6 effectively decreasing the number of NHEK that can occupy a culture flask.…”