Cytomorphological grading and feulgen DNA-analysis of metaplastic and neoplastic bronchial cells

Nasiell, M; Kato, Harubumi; Auer, Gert; Zetterberg, Anders; Roger, V.; Karlen, Lena

doi:10.1002/1097-0142(197804)41:4<1511::aid-cncr2820410440>3.0.co;2-i

Cited by 66 publications

(17 citation statements)

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“…Cytomorphology: The cytological criteria used are based on a grading system for squamous bronchial epithelium in Papanicolaoustained smear preparations developed at the Sabbatsberg Hospital, Stockholm, Sweden (1,10,(12)(13)(14). Squamous metaplastic and neoplastic bronchial cells were graded into regular squamous metaplastic cells, squamous metaplastic cells with mild, moderate and severe atypia, and cancer cells.…”

Section: Methodsmentioning

confidence: 99%

The significance of DNA measurements for the early detection of bronchial cell atypia

Ono

Auer

1983

Cytometry

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Investigations in an experimental model system and in clinical material indicate that squamous bronchial carcinoma is regularly preceded by the occurrence of bronchial cells with DNA values exceeding significantly the normal 4c region. The studies also show that cytomorphological methods do not allow the discrimination between milder forms of atypia and nonspecific cellular alterations. W e describe a combined cytomorphological and cytochemical diagnostic procedure which facilitates the distinction between atypical squamous cells and nonspecific cellular changes in the bronchial tree.

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Section: Methodsmentioning

confidence: 99%

The significance of DNA measurements for the early detection of bronchial cell atypia

Ono

Auer

1983

Cytometry

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“…The morphologic features of these tumor types have been reviewed elsewhere (75,84,105,188,203). A conversion of the normal pseudostratified columnar tracheobronchial epithelium (TBE) (103) to atypical forms of squamous metaplasias (i.e., mild, moderate, and severe (42,118,119,144,145)) precedes the appearance of cancers arising in bronchial epithelium. Quantitative methods (e.g., cytomorphology, chromatin texture) could be of value in further defining the stage of malignant progression of atypical squamous metaplasias (2,4,43,134).…”

Section: Morphologic Development Of Lung Cancermentioning

confidence: 99%

Intercellular Communication in Bronchial Epithelial Cells: Review of Evidence for a Possible Role in Lung Carcinogenesis

et al. 1990

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A challenging aspect of lung carcinogenesis is the elucidation of the mechanisms which permit initiated bronchial epithelial cells to attain a growth advantage over normal bronchial epithelial cells, and subsequently evolve into a malignant phenotype. In this review, the effects of interactions between normal and transformed cells, and the potential role of representative extrinsic factors on cell-cell communication are discussed. Evidence is presented to show how cell injury and the effects of serum and calcium may affect morphology and communication, and tumor development. A large number of autocrine-paracnne factors (e.g., TGFp, TGFa) are released by bronchial epithelial cells. These factors may inhibit or promote the proliferation of normal and transformed bronchial epithelial cells, respectively. The ability of certain injurious and tumor promoting agents (e.g., formaldehyde, TPA) to select for the transformed phenotype may involve selective cell injury, the induction of terminal differentiation and an inhibition of gap junction communication among normal BE cells.

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“…29,31 This has been used to facilitate the distinction between atypical squamous cells (precancer) and nonspecific cellular changes by measuring the nuclear DNA content of dysplastic bronchial epithelial cells. 30,32 The morphological criteria of dysplasia and carcinoma were applied for both cytology and DNA-image cytometry according to Saccomanno.…”

Section: Discussionmentioning

confidence: 99%

“…27,28 Diagnostic application in pulmonary pathology has been reported for identification of prospective malignant lesions (ie, dysplasia) and prediction of prognosis in manifest cancers. [29][30][31][32][33][34][35] . Both, FISH and DNA-image cytometry can be performed on the same specimen subsequent to a cytological diagnosis.…”

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confidence: 99%

Equivocal cytology in lung cancer diagnosis

Schramm

Wrobel

Born

et al. 2011

Cancer Cytopathology

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BACKGROUND:Sometimes, cytological lung cancer diagnosis is challenging because equivocal diagnoses are common. To enhance diagnostic accuracy, fluorescent in situ hybridization (FISH), DNA‐image cytometry, and quantitative promoter hypermethylation analysis have been proposed as adjuncts.METHODS:Bronchial washings and/or brushings or transbronchial fine‐needle aspiration biopsies were prospectively collected from patients who were clinically suspected of having lung carcinoma. After routine cytological diagnosis, 70 consecutive specimens, each cytologically diagnosed as negative, equivocal, or positive for cancer cells, were investigated with adjuvant methods. Suspicious areas on the smears were restained with the LAVysion multicolor FISH probe set (Abbott Molecular, Des Plaines, Illinois) or according to the Feulgen Staining Method for DNA‐image cytometry analysis. DNA was extracted from residual liquid material, and frequencies of aberrant methylation of APC, p16INK4A, and RASSF1A gene promoters were determined with quantitative methylation‐specific polymerase chain reaction (QMSP) after bisulfite conversion. Clinical and histological follow‐up according to a reference standard, defined in advance, were available for 198 of 210 patients.RESULTS:In the whole cohort, cytology, FISH, DNA‐image cytometry, and QMSP achieved sensitivities of 83.7%, 78%, 79%, and 49.6%, respectively (specificities of 69.8%, 98.2%, 98.2%, and 98.4%, respectively). Subsequent to cytologically equivocal diagnoses, FISH, DNA‐image cytometry, and QMSP definitely identified malignancy in 79%, 83%, and 49%, respectively. With QMSP, 4 of 22 cancer patients with cytologically negative diagnoses were correctly identified.CONCLUSIONS:Thus, adjuvant FISH or DNA‐image cytometry in cytologically equivocal diagnoses improves diagnostic accuracy at comparable rates. Adjuvant QMSP in cytologically negative cases with persistent suspicion of lung cancer would enhance sensitivity. Cancer (Cancer Cytopathol) 2011;. © 2011 American Cancer Society.

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Cytomorphological grading and feulgen DNA-analysis of metaplastic and neoplastic bronchial cells

Cited by 66 publications

References 4 publications

The significance of DNA measurements for the early detection of bronchial cell atypia

The significance of DNA measurements for the early detection of bronchial cell atypia

Intercellular Communication in Bronchial Epithelial Cells: Review of Evidence for a Possible Role in Lung Carcinogenesis

Equivocal cytology in lung cancer diagnosis

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