Cytoplasmic ABCG2 and Podoplanin Expression in Oral Squamous Cell Carcinoma Correlates with Lymph Node Metastasis

Abstract

The cervical lymph node metastasis of Oral Squamous Cell Carcinoma (OSCC) has been considered to be the main causes of death. The aim of our study was to clarify the expression pattern of ABCG2 and podoplanin in OSCC tissues and corresponding cervical lymph node, and evaluate the diagnostic value of ABCG2 and podoplanin in OSCC for tumorigenesis, histological stage and clinical prognosis. ABCG2 showed a mixed membranous and cytoplasmic pattern of staining in specifi c regions of stratum corneum and the center of well diff erentiated cancer nest, while only expressed in cytoplasm of poorly differentiated tumor nests. Podoplanin showed strong staining in LV endothelia, the periphery of well diff erentiated cancer nests, and tumor stromal fi broblasts surrounding tumor nests. The expression of ABCG2, especially cytoplasmic ABCG2, and podoplanin protein showed signifi cantly higher tendency to lymph node metastasis (P<0.05). Meanwhile, podoplanin positive rate increased with the decreasing degree of histological diff erentiation (P<0.05). ABCG2 and podoplanin expression pattern is correlated with lymph node metastasis of OSCC. This result suggested that cytoplasmic ABCG2 with podoplanin have clinical potential in reliable molecule diagonsis for OSCC.

Prognostic and clinicopathological significance of podoplanin immunoexpression in oral and oropharyngeal squamous cell carcinoma: A systematic review

“…The search strategy resulted in 721 records without duplicates. After full‐text reading, 58 studies were excluded (Appendix ) and 29 7,8,10,20‐45 were included (Figure 1). The same tissue samples were used in two included studies 23,29 (confirmed by personal communication, Dr Denise Oliveira, April 30, 2019).…”

“…A total of 2173 tumors were investigated across the 29 included studies. Males were usually more affected by OSCC than females 7,8,10,21‐31,33‐36,39‐42,44,45 . The participants' age ranged from 19 42 to 96 years, 36 with a mean age ranging from 50 39 to 69 years 22 .…”

“…The participants' age ranged from 19 42 to 96 years, 36 with a mean age ranging from 50 39 to 69 years 22 . Twenty‐two studies included intraoral tumors only, 7,8,10,20‐22,24‐28,32,33,35‐39,41‐43,45 two lip only, 23,29 two lip and intraoral, 31,34 two oropharyngeal only, 40,44 and one oropharyngeal and intraoral tumors 30 (Appendix ).…”

“…Twenty studies investigated histological grade, 7,20‐23,25‐30,33‐39,43,45 from which six found a significant association between less differentiated tumors and positive/high PDPN expression 7,25,26,28,33,35,38 . De Vicente et al 26 showed that well and moderately differentiated intraoral tumors presented higher expression of PDPN in the invasive front, while in poorly differentiated tumors, the expression of PDPN was more frequently difuse.…”

“…For histopathological grade analysis, “events” were considered moderately and poorly differentiated tumors and “non‐events” well‐differentiated, due to data grouping performed in the most of the studies, which resulted in nine studies included in this analysis 22,25,26,33,35‐37,39,45 . Three studies showed a significant positive association 25,26,33 and one 37 a significant negative association between PDPN expression and histological grade (Figure ).…”