Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling

Liu, Shu Chen; Hsu, Tien; Chang, Yu Sun; Chung, An Ko; Jiang, Shih Sheng; OuYang, Chun Nan; Yuh, Chiou-Hwa; Hsueh, Chuen; Liu, Ya Ping; Tsang, Ngan Ming

doi:10.1038/s41467-018-07660-6

Cited by 29 publications

(29 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LIF can maintain stem cell self‐renewal through the YAS–YAP–TEAD signaling pathway by binding membrane LIFR in embryonic stem cells 38 . LIF also enhanced NPC dissemination and invasion through promotion of nuclear YAP accumulation 39 . In GC, some factors were found to promote carcinogenesis by increasing nuclear YAP 33,40,41 .…”

Section: Discussionmentioning

confidence: 99%

Leukemia inhibitory factor promotes gastric cancer cell proliferation, migration, and invasion via the LIFR–Hippo–YAP pathway

Bian

Yang

Liang

et al. 2020

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

The long‐term outcome of gastric cancer (GC) patients remains unsatisfactory despite some recent improvements. Leukemia inhibitory factor (LIF) is a prognostic biomarker for some solid tumors, however its role in GC remains unknown. In this study, we demonstrated that LIF and LIF receptor (LIFR) are overexpressed in GC tissues and established that a correlation exists between them. LIF and LIFR expression are associated with tumor differentiation, lymphovascular invasion, tumor stage, lymph node metastasis, and pTNM stage, indicating that they may be useful prognostic factors. LIF promoted GC cell proliferation, colony formation, invasion, migration, and tumor growth; it also promoted cell cycle progression and inhibited apoptosis; and knocking out the LIFR gene reversed the effects of LIF. LIF inhibited the activity of the Hippo pathway, resulting in reduced phosphorylation of YAP, increased YAP nuclear translocation, and increased cell proliferation. Finally, silencing YAP mRNA expression suppressed cell proliferation. Overall, the results demonstrate that LIF promotes the malignant biological behavior of GC cells through LIFR–Hippo–YAP signaling. LIF may therefore be a useful biomarker for GC.

show abstract

Section: Discussionmentioning

confidence: 99%

Leukemia inhibitory factor promotes gastric cancer cell proliferation, migration, and invasion via the LIFR–Hippo–YAP pathway

Bian

Yang

Liang

et al. 2020

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

show abstract

“…The correlation between these pathway and tumor progression, including NPC, has been proven in previous studies. Especially, focal adhesion (Liu et al, 2018;Yuan et al, 2019;Yu et al, 2019), apoptosis (Li M. et al, 2019;Liang et al, 2019;Wang et al, 2019;Xie et al, 2019), and the tumor necrosis factor (TNF) signaling pathway (Lu et al, 2011;Ou et al, 2015;Huang et al, 2017;Deng et al, 2018) have been reported to be closely related to NPC development.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression and Alternative Splicing of Transcripts Associated With Cisplatin-Induced Chemoresistance in Nasopharyngeal Carcinoma

et al. 2020

View full text Add to dashboard Cite

Radiotherapy and adjuvant cisplatin (DDP) chemotherapy are standard administrations applied to treat nasopharyngeal carcinoma (NPC). However, the molecular changes and functions of DDP in NPC chemo-resistance remain poorly understood. In the present study, transcriptomic sequencing between 5-8F and 5-8F/DDP cells was performed to identify differential expression and alternative splicing (AS) characteristics in DDP-resistant NPC cells. Transcriptomic profiling identified 1,757 upregulated genes and 1,473 downregulated differentially expressed genes (DEGs). Bioinformatic analysis revealed that these DEGs were associated with or participated in important biological regulatory functions in NPC. Validation of 20 significant DEGs using quantitative real-time reverse transcription PCR showed that the expression patterns of 17 mRNAs were in accordance with the sequencing data. Intron retention was identified as the major AS event in chemoresistant cells. Furthermore, the expression level of matrix metalloproteinase 1 (MMP1), which was one of the most upregulated mRNAs in the chemoresistant cell lines, was significantly associated with the migration, invasion, and proliferation of NPC cells in vitro. Our study revealed that dysregulated genes and AS-mediated DDP chemoresistance might play important roles in NPC development and progression. Targeting aberrantly expressed genes might clarify the pathogenesis of NPC and contribute to developing new therapeutic strategies for NPC.

show abstract

“…#8418S) 19 , YAP (1:1000 dilution, Cell Signaling Technology, Cat. #14074S) 56 , and GAPDH (1:10,000 dilution, Cell Signaling Technology, Cat. #2118S).…”

Section: Methodsmentioning

confidence: 99%

A new, easily generated mouse model of diabetic kidney fibrosis

Zhang

Tolosa

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Our understanding of diabetic kidney disease pathogenesis has been hampered by the lack of easily generated pre-clinical animal models that faithfully recapitulate critical features of human disease. While most standard animal models develop manifestations of early stage diabetic injury such as hyperfiltration and mesangial matrix expansion, only a select few develop key late stage features such as interstitial fibrosis and reduced glomerular filtration rate. An underlying theme in these late stage disease models has been the addition of renin-angiotensin system hyperactivation, an important contributor to human disease pathogenesis. Widespread use of these models has been limited, however, as they are either labour intensive to generate, or have been developed in the rat, preventing the use of the many powerful genetic tools developed for mice. Here we describe the Akita +/− Ren +/− mouse, a new, easily generated murine model of diabetic kidney disease that develops many features of late stage human injury, including not only hyperglycemia, hypertension, and albuminuria, but also reduced glomerular filtration rate, glomerulosclerosis, and interstitial fibrosis.

show abstract

Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling

Cited by 29 publications

References 63 publications

Leukemia inhibitory factor promotes gastric cancer cell proliferation, migration, and invasion via the LIFR–Hippo–YAP pathway

Leukemia inhibitory factor promotes gastric cancer cell proliferation, migration, and invasion via the LIFR–Hippo–YAP pathway

Differential Expression and Alternative Splicing of Transcripts Associated With Cisplatin-Induced Chemoresistance in Nasopharyngeal Carcinoma

A new, easily generated mouse model of diabetic kidney fibrosis

Contact Info

Product

Resources

About