Cytoprotective Effect of Ethanol Extract from Maesil (Prunus mume Sieb. et Zucc.) on Alloxan-induced Oxidative Damage in Pancreatic-cell, HIT-T15

Abstract

-The present study was designed to examine the potential antidiabetic and antioxidant effect of ethanol extract from Prunus mume fruit (PME) against alloxan-induced oxidative stress in pancreatic -cells, HIT-T15. To evaluate the antidiabetic effect of PME, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliu bromide (MTT) cell proliferation assay, lactate dehydrogenase (LDH) release assay, NAD + /NADH ratio and insulin secretion were assessed. We also measured its antioxidant effect against alloxan-induced oxidative stress in the cells by assessing the levels of the antioxidant enzymes including superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx). The results of this analysis showed that alloxan significantly decreased cell viability, increased LDH leakage, and lowered NAD + /NADH ratio and insulin secretion in HIT-T15 cells. However, PME significantly increased the viability of alloxan-treated cells and lowered LDH leakage. The intracellular NAD + /NADH ratio and insulin secretion were also increased by 1.5~1.9-fold and 1.4-fold, respectively, after treatment with the PME. The HIT-T15 cells treated with alloxan showed significant decreases in the activities of antioxidant enzymes, while PME significantly elevated the levels of antioxidant enzymes. Based on these results, we suggest that PME could have a protective effect against the cytotoxicity and dysfunction of pancreatic β-cells in the presence of alloxan-induced oxidative stress.

Quantitation of Phenolic Compounds Related to Antioxidant and Antiosteoporosis Activities in Ripe and Unripe Maesil (Prunus mume)

“…Also, the unripe maesil treated by smoking or steaming process is called as ome in Korea, and it has been used as a traditional medicine for the treatment of emetic, tussis, sputum, diarrheal, and pyretic disease for long time [1]. And maesil has been reported to have various biological activities such as antioxidant [2][3][4][5][6][7][8][9][10][11], antimicrobial, and antibacterial [12][13][14][15], mitogenesis [16], inhibition of growth signals of vascular smooth muscle cells [17], apoptosis [18], anti-inflammatory activity [19,20], anticancer cell [9,21], immune function enhancing, therapeutic activities for cognitive impairments by Alzheimer's disease [22], cytoprotective [23], antidiabetic activity [24], antiobesity activity [25], and antiosteoporosis activity [26,27].…”