Cytoskeleton and polyploidy after maturation and fertilization of cryopreserved germinal vesicle—stage mouse oocytes

Eroğlu, Ali; Toner, Mehmet; Leykin, Lucy; Toth, Thomas L.

doi:10.1007/bf02744940

Cited by 46 publications

(24 citation statements)

References 23 publications

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“…Oocyte cryopreservation at the GV stage seems to be particularly advantageous to circumvent the spindle damage, because increased digyny often experienced after cryopreservation of M-II stage oocytes [65]. In pigs, because of low survival rates, cryopreservation of immature oocytes such as at the GV stage gained little interest so far.…”

Section: Immature Oocytes; Low Survival But the Developmental Abilitymentioning

confidence: 99%

Factors Affecting Cryopreservation of Porcine Oocytes

2012

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Section: Immature Oocytes; Low Survival But the Developmental Abilitymentioning

confidence: 99%

Factors Affecting Cryopreservation of Porcine Oocytes

2012

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“…In the limited studies that have been done in the mouse, it has been shown that GV-stage oocytes can be successfully frozen and thawed [31][32][33], that they can subsequently mature in culture and form morphologically normal spindles [33], and that they can develop after fertilization and produce live births [31,32]. However, as in bovine oocytes, the developmental competence in terms of blastocyst formation and live births is approximately 50% lower than that of unfrozen controls [31,32].…”

Section: Introductionmentioning

confidence: 97%

Maturation, Fertilization, and the Structure and Function of the Endoplasmic Reticulum in Cryopreserved Mouse Oocytes1

Lowther

Weitzman

Maier

et al. 2009

Biology of Reproduction

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Oocyte cryopreservation is a promising technology that could benefit women undergoing assisted reproduction. Most studies examining the effects of cryopreservation on fertilization and developmental competence have been done using metaphase IIstage oocytes, while fewer studies have focused on freezing oocytes at the germinal vesicle (GV) stage, followed by in vitro maturation. Herein, we examined the effects of vitrifying GVstage mouse oocytes on cytoplasmic structure and on the ability to undergo cytoplasmic changes necessary for proper fertilization and early embryonic development. We examined the endoplasmic reticulum (ER) as one indicator of cytoplasmic structure, as well as the ability of oocytes to develop Ca 2+ release mechanisms following vitrification and in vitro maturation. Vitrified GV-stage oocytes matured in culture to metaphase II at a rate comparable to that of controls. These oocytes had the capacity to release Ca 2+ following injection of inositol 1,4,5-trisphosphate, demonstrating that Ca 2+ release mechanisms developed during meiotic maturation. The ER remained intact during the vitrification procedure as assessed using the lipophilic fluorescent dye DiI. However, the reorganization of the ER that occurs during in vivo maturation was impaired in oocytes that were vitrified before oocyte maturation. These results show that vitrification of GV-stage oocytes does not affect nuclear maturation or the continuity of the ER, but normal cytoplasmic maturation as assessed by the reorganization of the ER is disrupted. Deficiencies in factors that are responsible for proper ER reorganization during oocyte maturation could contribute to the low developmental potential previously reported in vitrified in vitro-matured oocytes.assisted reproductive technology, gamete biology, in vitro fertilization, meiosis

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“…In pigs, it has been confirmed that mature (MII stage) oocytes have higher survival ability during cryopreservation than immature oocytes [21]. On the other hand, cryopreservation of matured mammalian oocytes has been associated with damage of the meiotic spindle and actin microfilaments [2,3,11,19], causing the failure of second polar body (2PB) extrusion and the possible generation of embryos with abnormal numbers of chromosomes [2,6,7]. Cytoskeletal elements are known to regulate several events during fertilization in mammalian oocytes such as sperm penetration and 2PB extrusion [25,26].…”

mentioning

confidence: 98%