Cytosolic factors which affect microsomal lipid peroxidation in lung and liver

Wright, Jo Rae; Colby, Howard D.; Miles, P. R.

doi:10.1016/0003-9861(81)90095-3

Cited by 422 publications

(175 citation statements)

References 38 publications

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“…Lipid per-oxidation was analyzed by the determination of thiobarbituric acid reactive substnces (TBARS) 16 with some modifications 17 and GSH contents liver tissue homogenate by using the method of.…”

Section: Lipid Peroxidation Assessmentmentioning

confidence: 99%

Protective Effect of Aerva jevanica Against Ethanol Induced Hepatic Stress in Rats: A Randomized Control Report

Khan¹

2017

IJPER

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Background: Aerva jevanica (AJME) possess antioxidant activity and hepatoprotective effects, and traditionally in Pakistan for liver diseases. Methods: 24 male albino rats were divided into five groups of 4 chicks of each, having free access to food and water. Group I (control) while group II, III and IV were treated with ethanol 24 hrs for 2 weeks. Rats group II received only ethanol group III received silymarin at a dose of 50 mg/kg while group IV was given AJME (200 mg/kg). The activities of alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and were determined in serum. Catalase (CAT), peroxidase (POD), glutathione-S transferase (GST), glutathione peroxidase (GSH-Px), glutathione reductase (GSR) activity was measured in liver homogenates. Thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) concentration were also evaluated in liver homogenates. Results: Hepatotoxicity induced with ethanol was evidenced by significant increase in lipid peroxidation (TBARS) and serum, ALT, ALP, LDH. Level of GSH determined in liver was significantly reduced, as were the activities of antioxidant enzymes; CAT, POD, GSH-Px, GSR, GST treated with ethanol. AJME (200 mg/kg bw) and silymarin (50 mg/kg bw) co-treatment prevented all the changes observed with ethanol-treated rats. Conclusion: The results of present work indicate that AJME has a significant protective effect against ethanol induced hepatotoxicity in chicks, which may be due to their antioxidant potential.

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Section: Lipid Peroxidation Assessmentmentioning

confidence: 99%

Protective Effect of Aerva jevanica Against Ethanol Induced Hepatic Stress in Rats: A Randomized Control Report

Khan¹

2017

IJPER

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“…Creatinine was estimated by the method of Hare (1950). Membrane lipid peroxidation LPO was done following the modified protocol of Wright et al (1981). Reduced glutathione was measured by the method of Jollow et al (1974).…”

Section: Biochemical Measurementsmentioning

confidence: 99%

Toxicity Evaluation of Crop Plants Irrigated with Treated Municipal Wastewater

Dhir¹,

Nasim²,

Nafees³

et al. 2015

Research J. of Environmental Toxicology

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In the present investigation, tomato plants irrigated with treated wastewater were evaluated for their toxicity using short term (acute) animal studies. Male wistar rats fed with tomato plants at the dosage of 150 and 300 mg kgG 1 b.wt. for 14 consecutive days showed no symptoms of toxicity. Assessment of haematological parameters such as RBC, WBC, haemoglobin, platelet count showed no significant changes. Plasma and serum analysis also indicated no significant differences in parameters such as urea, AST, ALT, ALP, bilirubin in all the treatments. The present investigations suggested that short term wastewater irrigation in plants do not pose any toxicity to animals. The toxicity depends upon the level of composition of treated wastewater and its transfer to the soil and then plants. Further studies are required to assess toxic effect of long term irrigation with treated wastewater.

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“…Estimation of LPO LPO was estimated by the method of Wright et al (1981). The reaction mixture, in a total volume of 2.0 mL, contained 1.8 mL phosphate buffer (0.1 M, pH 7.4), 0.2 mL of liver homogenate (10% w/v).…”

Section: Assay For Serum Alt Activitymentioning

confidence: 99%

Protection against experimental salmonellosis by recombinant 49 kDa OMP of Salmonella enterica serovar Typhi: biochemical aspects

Kaur

Jain

2013

Biologia

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Typhoid fever is systemic illness caused by Salmonella enterica serovar Typhi (S. Typhi) in humans. Increasing multidrug resistant strains of S. Typhi and limited effect of available vaccines has necessitated exploring of new immunogens for protection against it. Earlier studies have shown that a crude preparation of outer membrane proteins (OMPs) of S. Typhi evokes strong immune response and induces a protective immunity against infection caused by diverse Gram-negative bacteria. In the present study we have evaluated the protective effect of a purified recombinant 49 kDa (r49kDa) OMP of S. Typhi alone or along with alum or complete Freund's adjuvant, against a challenge by S. Typhi (0.4 × 50% lethal dose) by biochemical estimation of serum enzymes and oxidative stress enzymes in Swiss albino mice. There was a decrease in activity of alanine aminotransferase by 14.28%, 38.09%, 23.80%; aspartate aminotransferase by 6.25%, 25%, 16.25%; lipid peroxidation by 4.34%, 18.84%, 11.59%; and catalase by 8%, 14%, 10%, respectively, whereas increase in activity of reduced glutathione by 33.33%, 61.11%, 44.44%; glutathione peroxidase by 7%, 16%, 10%; and glutathione reductase by 8%, 20%, 12%, respectively, as compared to control animals challenged with bacteria without pre-immunization. The results indicated that immunization of mice with r49kDa OMP alone or in combination with adjuvants protected and normalized the liver. It reduces the development of oxidative stress in mice against Salmonella infection and the risk of getting typhoid. These results represent an additional supplement to our earlier reported data on protective immunity evoked by this protein.

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Cytosolic factors which affect microsomal lipid peroxidation in lung and liver

Cited by 422 publications

References 38 publications

Protective Effect of Aerva jevanica Against Ethanol Induced Hepatic Stress in Rats: A Randomized Control Report

Protective Effect of Aerva jevanica Against Ethanol Induced Hepatic Stress in Rats: A Randomized Control Report

Toxicity Evaluation of Crop Plants Irrigated with Treated Municipal Wastewater

Protection against experimental salmonellosis by recombinant 49 kDa OMP of Salmonella enterica serovar Typhi: biochemical aspects

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