Cytotoxic Activity, and Molecular Docking of Indole Alkaloid Voacangine and Bisindole Alkaloid Vobtusine, Vobtusine Lactone from the Indonesian Plant: Voacanga foetida (Blume) Rolfe

Abstract

ABSTRACT
 The purpose of this study was to isolate and test its cytotoxic activity starting from extract fraction and its isolate compound, then carried out molecular docking to confirm the potential biological activity of ligands (vocangine, vobtusine, and vobtusine lactone) against inhibition proteins (Bcl-2, Bcl-xL, and Mcl-1), activation protein Bax and activation of apoptotic execution protein Caspase-3. This research is an experimental quantitative study using column chromatography and HPLC methods in the isolation process, MTT assay in determining the cytotoxic activity, and molecular docking in determining the prediction of apoptotic mechanism. The cytotoxic activity of VFB-DA, VFB-DB, VFB-BuOH, VFB-DB4 fractions, voacangine compounds, vobtusine are very strong. while vobtusine lactone is moderate cytotoxic activity. The docking score for voacangine, vobtusine, and vobtusine lactone compounds against Bcl-2 is -9.93; -10.07; -9.03 kcal/mol, against Bcl-xl is -9.77; -11.69; -9.76 kcal/mol, against Mcl-1 is -10.70; -10.77; -9.53 kcal/mol, and for Bax is -8.99; -6.87; -6.99 kcal/mol, as well as against caspase3 is -12.05; -12.21; -12.02 kcal/mol. The cytotoxic activity of voacangine, vobtusine, and vobtusine lactone compounds is thought to cause cell death by suppressing Bcl-2 activity; Bcl-xl; and Mcl-1, increased Bax activity and increased caspase3 activity.
 
 Key words: Voacanga foetida, in vitro, in silico

Isolation and Cytotoxic Activity Test of Alkaloids from Dichloromethane Fraction of Bark of Tampa Badak (Voacanga foetida (Blume) Rolfe) Against T47D Cell Line

“…Some isolated compounds from this plant such as voacristine, voacangine, lombine, and coronaridine were reported by Hadi to have antibacterial activity [6]. Our recent works also reported another alkaloids, i.e vobtusine and vobtusine lactone,two aspidosperma bis-indole alkaloids with potential cytotoxic activity [3], [4].…”

“…Estimated more than 2000 compounds classified as alkaloids [2]. Two bis-indole alkaloids from Voacanga foetida (Blume) Rolfe have been reported to have cytotoxic activity against HL60 [3] and A549 cell lines [4]. Alkaloid compounds as anticancer agents work by inducing apoptosis, inhibiting metastasis, inhibiting the cell cycle, inducing autophagy, inhibiting angiogenesis, inducing necrosis, activating mitochondrial pathway, and playing a role in tumor development [5].…”

Cytotoxic Activity of Ethyl Acetate Extract from Voacanga foetida (Bl.) Rolfe Leaves Against T47D Breast Cancer Cells

“…The greater the absorbance, the greater the number of living cells. The cytotoxicity assay using MTT assay followed the standard protocol from the previous literature 17 . In this work, in vitro cytotoxic activity of the ethyl acetate extract at various concentrations (10, 1, and 0.1 μg/mL) and various incubation times were evaluated against the T47D breast cancer cell line, and the IC50 values were calculated.…”