Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract

Zubair, Muhammad Sulaiman; Anam, Syariful; Lallo, Subehan

doi:10.1016/j.apjtb.2016.04.014

Cited by 14 publications

(8 citation statements)

References 17 publications

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“…Recent studies on different solvent extracts of this plant showed the presence of secondary metabolites like tannins, terpenoids, cardiac glycosides, steroids, phlobatannins, saponins. However, alkaloids and steroids are also known to show cytotoxicity by inhibiting cellular DNA in a concentration [17].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

Nasrin¹,

Afroz²,

Sharmin³

et al. 2019

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Commelina diffusa Burm. F. is a herbaceous tropical plant with different traditional medicinal uses. Present study is aimed to isolate the bioactive compounds from DCM-Methanol extract of the powdered whole plant of Commelina diffusa and to investigate the cytotoxic, antimicrobial and antioxidant activities of the crude extract and its twelve vacuum liquid chromatographic fraction (CD 1-12 ). Vacuum Liquid Chromatography (VLC) was employed to isolate bioactive metabolites. The physicochemical properties of the isolated compound were examined and the molecular structure was elucidated by NMR spectroscopy. Cytotoxic, antibacterial, antioxidant activities were evaluated following brine shrimp lethality bioassay, disc diffusion method and DPPH free radical scavenging assay respectively. The isolated compound was identified as steroid (stigmasterol) which had significant cytotoxic effect on vero cell line. The crude extract and its fractions (CD8-CD12) exhibited strong cytotoxicity in brine shrimp lethality bioassay having LC 50 values 3. 79, 9.19, 29.49, 16.60, 19.36, 44.58 μg/mL respectively. The crude extract showed mild to strong antibacterial activity. Fractions (CD5-CD12) showed mild to strong antibacterial activity against Escherichia coli in comparison with Kanamycin (standard). Strong antioxidant activities were found in crude extract (IC 50 value 30.52 μg/ml), CD11 (IC 50 value 39.27 μg/ml) and CD12 (IC 50 value 19.50 μg/ml). The study suggests Commelina diffusa plant extract to have strong antioxidant and cytotoxic activity which is indicative of presence of compounds with broad spectrum of curative applications. One compound namely stigmasterol has been isolated from the plant.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

Nasrin¹,

Afroz²,

Sharmin³

et al. 2019

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“…Cytotoxic activity was applied to the Human ductal breast epithelial tumor cell line (T47D), human cervical cancer cell line (HeLa), and green African monkey renal epithelial cell (Vero) cell lines by the MTT method as described in our previous study [12]. The stock samples were briefly diluted with RPMI-1640 medium to desired concentrations of 25, 12.5, 6.25, 3.125, 1.5625, 0.7812, and 0.3906 μg/mL.…”

Section: Cytotoxicity Testmentioning

confidence: 99%

In Vitro and In Silico Studies of Quercetin and Daidzin as Selective Anticancer Agents

et al. 2021

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Quercetin and daidzin are flavonoid and flavonoid glycoside type compounds that have been found in many plants and nutraceuticals. This study aims to examine the in vitro cytotoxic and selectivity properties of quercetin and daidzin on breast and cervical cancers and to study their molecular interaction and stability on epidermal growth factor receptor tyrosine kinase (EGFR-TK) by applying molecular docking and molecular dynamics (MD) simulations. In vitro anticancer activity was performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method on breast cancer cell (T47D), cervical cancer cells (HeLa), and Vero normal cells, while molecular docking and MD simulation were done by using AutoDock Vina and Amber18 package software, respectively. Quercetin and daidzin showed potent cytotoxic and high selectivity on both cell lines. Daidzin was found to has a higher IC50 and selectivity index than quercetin. Docking and MD results showed that both compounds prefer to interact with epidermal growth factor receptor tyrosine kinase (EGFR-TK). Daidzin showed better interaction than quercetin with a docking score of -9.6 kcal/mol. Also, daidzin was found more stable than quercetin with low RMSD and RMSF values.

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“…Kandungan bioaktif tumbuhan memainkan peranan penting dalam mengaruh pembaikan libido jantan. Kandungan alkaloid L. amara yang tinggi seperti quinoline, 3-dimethylallyl-2-quinolone, furokuinolin, 2-arilkuinolin dan 4-kuinolon (Sharma et al 2010;Zubair et al 2016)…”

Section: Analisis Aras Testosteronunclassified

Potensi Afrodisiak Lunasia amara ke atas Tikus Jantan Teraruh Diabetes

Rahmat

Kamalrudin

Fazry³

et al. 2018

JSM

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Potensi Afrodisiak Lunasia amara ke atas Tikus Jantan Teraruh Diabetes (Aphrodisiac Potential of Lunasia amara on Diabetic-Induced Male Rats) NOR-RAIDAH RAHMAT, AMIRA KAMALRUDIN, SHAZRUL FAZRY & MAHANEM MAT NOOR* ABSTRAK Diabetes melitus telah terbukti mengganggu penghasilan testosteron dan menyebabkan masalah libido dalam kalangan lelaki. Sehingga kini, tiada kajian mengenai potensi Lunasia amara dalam membaiki aktiviti seksual tikus jantan teraruh diabetes. Oleh itu, kajian ini dijalankan untuk mengenal pasti potensi afrodisiak L. amara ke atas tikus jantan teraruh diabetes. Empat kumpulan tikus teraruh diabetes masing-masing diberi perlakuan ekstrak L. amara (250 dan 500 mg/ kg berat tubuh), 500 mg/kg metformin dan air suling. Tikus kumpulan kawalan normal tanpa aruhan diabetes menerima perlakuan air suling. Perlakuan diberikan secara suap paksa selama 30 hari untuk melihat kesan L. amara ke atas status libido, aras testosteron serum, berat tubuh tikus, morfometri testis dan epididimis kauda serta aktiviti enzim antioksida testis tikus teraruh diabetes berbanding kawalan. Keputusan kajian menunjukkan berlaku penurunan libido, aras testosteron dan aktiviti khusus enzim antioksida (glutation peroksidase, katalase dan superoksida dismutase) testis tikus teraruh diabetes secara signifikan (p<0.05) pada kedua-dua dos tersebut berbanding kawalan normal. Sementara itu, perlakuan L. amara didapati tidak menjejaskan morfometri testis, epididimis kauda dan berat tubuh tikus yang menerima perlakuan L. amara berbanding kawalan normal. Kajian ini membuktikan bahawa ekstrak akuas batang L. amara pada dos 250 dan 500 mg/kg berat tubuh tidak berupaya memperbaiki aktiviti seksual tikus jantan teraruh diabetes. ABSTRACT Accumulating evidence shows that diabetes mellitus alters the production of testosterone and contributes to the reduction of libido in male. Formerly, no study has been conducted on the effect of Lunasia amara on sexual activity in diabeticinduced male rats. Therefore, present study looked into the aphrodisiac potential of L. amara on diabetic-induced male rats. Four groups of diabetic-induced male rats were given L. amara aqueous extract (250 and 500 mg/kg body weight), 500 mg/kg metformin and distilled water, respectively. A group of control rats was given distilled water. Treatments were given for 30 days to investigate the effect of L. amara aqueous extract on libido, serum testosterone level, body weight, morphometry of testis, cauda epididymis and antioxidant enzymes activity in diabetic-induced rats in comparison to control. The result showed diabetic-induced rats treated with both doses of L. amara extract exhibit significant reduction (p<0.05) in libido, testosterone level, testis antioxidant enzymes activity (glutathione peroxidase, catalase and superoxide dismutase) compared to control. On the other hand, the administration of L. amara did not affect body weight, morphometry of testis and cauda epididymis of diabetic-induced rats compared to normal control. This findings suggested that L. amara at ...

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Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract

Cited by 14 publications

References 17 publications

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

In Vitro and In Silico Studies of Quercetin and Daidzin as Selective Anticancer Agents

Potensi Afrodisiak Lunasia amara ke atas Tikus Jantan Teraruh Diabetes

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Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract

Cited by 14 publications

References 17 publications

Cytotoxic, Antimicrobial and Antioxidant Properties of &lt;i&gt;Commelina diffusa&lt;/i&gt; Burm. F.

Cytotoxic, Antimicrobial and Antioxidant Properties of &lt;i&gt;Commelina diffusa&lt;/i&gt; Burm. F.

In Vitro and In Silico Studies of Quercetin and Daidzin as Selective Anticancer Agents

Potensi Afrodisiak Lunasia amara ke atas Tikus Jantan Teraruh Diabetes

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Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.

Cytotoxic, Antimicrobial and Antioxidant Properties of <i>Commelina diffusa</i> Burm. F.