Cytotoxic and anticancer activity of a novel synthesized tet-AuNPs simultaneously activates p53 and inhibits NF-kB signaling in SKBR3 cell line

Abstract

Objective The objective of this study was to synthesize novel gold nanoparticles (Au(0)NPs) that simultaneously activates p53 and inhibits NF-kB signaling in SKBR3 breast cancer cells. Methods The tetracycline-based Au(0)NPs were synthesized by chemical method. These Au(0)NPs were characterized by different authentic techniques. The first characterization technique was UV-Visible (UV-Vis) spectroscopy to monitor Plasmon absorption maxima at 529 nm. The second technique was X-ray powder diffraction (XRD) pattern to confirm the crystalline nature of the prepared the Au(0)NPs. Finally, the Fourier transformed infra-red (FT-IR) spectroscopy was used to study the bonding patterns of the prepared the prepared Au(0)NPs with a size range of 10-50 nm. These Au(0)NPs were used as a nanomedicine to treat SKBR3 breast cancer cells using relative gene and protein expression studies. Results In response to Au(0)NPs, the level of caspases (3, 8 and 9) were changed, p53 was activated and NF-κB was inhibited simultaneously. The viability and proliferation of breast cancer cells (SKBR3) were downregulated as compared to the normal breast cells (CRL-4010). In addition, the gene and protein expressions of caspases supported the data. The inverse relationship between p53 and NFκB was found in AuNPs treated breast cancer cells. Conclusions The study laid down a preliminary step to employ newly synthesized AuNPs as a chemotherapeutic agent that stimulated cell death via both intrinsic and extrinsic apoptosis and inhibited the cell survival via suppressing the NFκB and recommended to have better insight.

Anti-Inflammatory and Antiatopic Effects of Rorippa cantoniensis (Lour.) Ohwi in RAW 264.7 and HaCaT Cells

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Polyopes affinis Suppressed IFN-γ- and TNF-α-Induced Inflammation in Human Keratinocytes via Down-Regulation of the NF-κB and STAT1 Pathways

“…Previous studies have shown that IFN-γ and TNF-α can activate NF-κB, which is responsible for the expression of pro-inflammatory chemokines and cytokines [ 42 ]. Among these cytokine stimuli, IκB proteins are phosphorylated and degraded, which allows the translocation of NF-κB into the nucleus, where it can bind to specific promoter regions of target genes and activate the expression of inflammatory-cytokine genes [ 43 ]. Thus, the inhibition of NF-κB activation plays a key anti-inflammatory role in AD.…”

Light-Responsive Layer-By-Layer Film Containing Gold Nanorods for Sequential Drug Release

“…Using controlled and sequenced release mechanisms to prevent undesirable outcomes associated with simultaneous codelivery, local DDSs can restrict drug–drug interactions, improve therapeutic effectiveness, and minimize drug resistance in skin cancer treatment. − The field of sequential and on-demand DDSs has seen a growth in the development of responsive biomaterials with configurable physical and chemical characteristics. − Because of their reversible phase transformation in reaction to stimulation and physiochemical influences such as pH, temperature, and enzymatic activity, and external forces such as UV light, near-infrared (NIR), ultrasound, electric, and magnetic radiation, these materials can be used as drug-carrier frameworks for the effective delivery of pharmaceuticals. − Because most drugs must be given repeatedly and consistently over long periods of time to be effective, and their concentrations must remain within the desired effective range to prevent overuse or underdosing, it is necessary to develop and improve new adaptive systems that can be easily controlled externally and capable of being spatially programmed. ,, …”