Cytotoxic and Enzyme Inhibitory Potential of Two Potentilla species (P. speciosa L. and P. reptans Willd.) and Their Chemical Composition

Abstract

In this work, the biological and chemical fingerprints of three extracts (ethyl acetate, methanol, and water) from two Potentilla species (Potentilla reptans and P. speciosa) were investigated. Antioxidant, enzyme inhibitory, and cytotoxic activities were performed for the biological fingerprint. For the chemical characterization, total bioactive components, and individual phenolic components were determined using photometric and HPLC methods, respectively. The main identified phenolic compounds in these extracts were rutin and catechin. Methanol and water extracts contained the highest total phenolic and flavonoid content. The results of antioxidant assays showed that methanol and water extracts displayed higher antioxidant activity compared to the ethyl acetate extract. Generally, methanol and water extracts exhibited higher biological activities correlated with higher levels the bioactive components. For P. speciosa, the methanol extract exhibited the highest enzyme inhibitory activity (except BChE inhibitory activity). P. reptans exhibited also high antiproliferative activity against MCF-7 cells whilst P. speciosa had weak to moderate activity against both of A549 and MCF-7 cell lines. The results suggest that Potentilla species could be potential candidates for developing new phyto-pharmaceuticals and functional ingredients.

Untargeted metabolomic profiling of three Crataegus species (hawthorn) and their in vitro biological activities

“…The in vitro enzyme inhibitory effects of extracts on four enzymes, that are α ‐amylase, α ‐glucosidase, cholinesterases, and tyrosinase were evaluated, as previously reported . The enzyme inhibitory actions of extracts were assessed as equivalents of kojic acid (KAE) for tyrosinase, galantamine for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and acarbose for α ‐amylase and α ‐glucosidase.…”

“…The findings were given as standard compounds equivalents of EDTA or Trolox (mg EDTAE g −1 and mg TE g −1 respectively). The assay methods were given in our earlier work …”

Chemical profiling and pharmaco‐toxicological activity ofOriganum sipyleumextracts: Exploring for novel sources for potential therapeutic agents

“…Total of phenols (TPC) (by standard Folin–Ciocalteu method) and flavonoids (TFC) (by AlCl 3 method) were assayed by standard Folin‐Ciocalteu and AlCl3 method, respectively (Uysal et al, ). TPC and TFC concentrations were expressed as equivalents of gallic acid (mg GAE/g) and rutin (mg RE/g), respectively.…”

“…The inhibitory effect of the extracts on tyrosinase, α‐amylase, α‐glucosidase, and cholinesterases was investigated through colorimetric methods, as previously reported (Uysal et al, ). Acarbose (against α‐amylase and α‐glucosidase), galantamine (against or AChE and BChE), and kojic acid (against tyrosinase) were used as reference inhibitors to calculate the enzyme inhibition activity of the extracts.…”

Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis

“…The findings were given as standard compounds equivalents of ethylenediaminetetraacetic acid (EDTA) or Trolox (EDTAE g ‐1 and TE g ‐1 , respectively). The assay methods were given in our earlier work …”