Cytotoxic, antitumor and leukocyte migration activities of resveratrol and sitosterol present in the hidroalcoholic extract of Cissus sicyoides L., Vitaceae, leaves

Abstract

RESUMO:Cissus sicyoides pertence à família das Vitaceae. É conhecido popularmente como "insulina, cipó-puca, bejuco caro, puci, anil trepador". Esta planta apresenta efeito vasoconstritor eatividade antibacteriana. No Brasil, C. sicyoides foi avaliado pelas suas propriedades anticonvulsivante e anti-diabética. Estudos fitoquímicos identificaram e isolaram a partir de suas partes aéreas o sitoesterol e o resveratrol compostos que são apontados por apresentar atividade antitumoral. O objetivo deste estudo foi investigar as atividades citotóxica e antitumoral do extrato hidroalcoólico Cissus sicyoides bem como a sua capacidade de recrutar leucócitos para os tecidos lesados. Cissus sicyoides não demonstrou atividade citotóxica, mas apresentou uma inibição do crescimento tumoral frente aos tumores testados. O extrato teve um forte efeito quimiotático 24 h após o tratamento. O extrato hidroalcoólico de Cissus sicyoides apresentou atividade antitumoral, relacionada ao recrutamento de linfócitos T para o local da lesão sugerindo que esta atividade esteve relacionada à ativação da linhagem linfóide. Unitermos:Cissus sicyoides, atividade antitumoral, migração de leucócitos, ativação da linhagem linfóide.ABSTRACT: Cissus sicyoides L. belongs to the Vitaceae family. It is popularly known as "insulina, cipo-pucá, bejuco caro, puci, anil trepador". A vasoconstrictor effect and an antibacterial activity have also been allocated to it. In Brazil, C. sicyoides was evaluated for its anticonvulsant and anti-diabetic properties. Phytochemistry studies identified and isolated sitosterol and resveratrol compounds from its aerial parts which are pointed out as having antitumor activities. The goal of this study was to investigate the cytotoxic and antitumor activities of Cissus sicyoides hydroalcoholic extract as well as its ability to repair leukocytes cells to injured tissue. Cissus sicyoides did not demonstrate cytotoxic activity but showed an inhibition of tumor growth in face of the tumors tested. The extract had a strong chemotactic effect on the 24 h period after treatment. The hydroalcoholic extract of Cissus sicyoides presented antitumor activity which was prompted by T lymphocytes recruitment to the local lesion and it suggests a new pathway to antitumor activity by activation of lymphoid lineage.

Bioassay-Guided Identification of the Antiproliferative Compounds of Cissus trifoliata and the Transcriptomic Effect of Resveratrol in Prostate Cancer Pc3 Cells

“…Hence, the compounds in the bioactive fraction might act in synergy and contribute to the antitumor properties associated with C. trifoliata in traditional medicine. According to other studies, the content of terpenes, flavonoids, and stilbenes plays a major role in the anticancer properties of plants from the genus Cissus [ 5 , 21 , 22 ]. For example, from the fractionation of the ethanolic extract of the aerial part of C. quadrangularis , a bioactive fraction against MCF7 breast cancer cells in vitro with a high content of the flavonoids quercetin and rutin was obtained [ 21 ].…”

“…For example, from the fractionation of the ethanolic extract of the aerial part of C. quadrangularis , a bioactive fraction against MCF7 breast cancer cells in vitro with a high content of the flavonoids quercetin and rutin was obtained [ 21 ]. The hydroalcoholic leaf extract from C. sicyoides showed inhibition of Ehrlich carcinoma in mice and the bioactivity was related to the content of sitosterol and resveratrol [ 22 ]. Some of the constituents of the bioactive fraction of C. trifoliata have been isolated from the stems of other species of the genus Cissus , such as ursolic and betulic acids in C. assamica [ 23 ] and apigenin in C. digitata [ 24 ], whereas kaempferol [ 25 ] and resveratrol have been found in C. quadrangularis [ 26 ].…”

Phytochemical screening and evaluation of cytotoxicity of stem bark extracts of <i>Anaxagorea dolichocarpa</i> and <i>Duguetia chrysocarpa</i> (Annonaceae)

“…Among the medical properties attributed to plants and a gamut of varieties encountered in tropical countries, among them Brazil, there is innumerable research with the objective of identifying efficient activities against cancer, from which several studies are being made to find active substances with anti-cancer activity [19].…”

O uso da planta Cissus Verticillata (Insulina) no tratamento do Diabetes Mellitus, em uma comunidade costeira do Pará, Amazônia, Brasil

“…Por outro lado, Alexandre (2007) destaca a tiramina como princípio ativo responsável pela ação hipoglicemiante na espécie. Em outros trabalhos pôde-se verificar que a C. verticillata apresenta ainda ações antibacterianas, anticonvulsivante, antitumoral, antioxidante, anti-inflamatória, antiepilética, anti-hipertensiva, antitérmica, antirreumática, hipotensora e fotohemolítica (Beltrame et al, 2002;Alexandre, 2007;Rosendo, 2009;Lucena et al, 2010;Madariaga et al, 2010;Feijó et al, 2012).…”