Cytotoxic constituents from Andrographis paniculata induce cell cycle arrest in jurkat cells

Abstract

Herbal medicines are now attracting attention as potential sources of anticancer agents. Andrographis paniculata is a traditionally used anticancer herb in Indian and Chinese herbal medicine. Phytochemical investigation of the ethanol extract of the aerial parts of this herb resulted in the isolation of 14 compounds including flavonoids and labdane diterpenoids. This is the first isolation of compound 6 from a natural source, and the aerial parts of A. paniculata are a rich source for the molecule andrographolide (9, 1.375%, w/w). The structures of the isolated compounds were established by means of spectral data. The cytotoxic activities of these isolates were evaluated against Jurkat, PC-3, HepG2 and Colon 205 tumor cells, and normal cells PBMCs. The bioactivity assays showed that metabolites 1-4 and 6-8 exhibited moderate cytotoxic activity against Jurkat, PC-3 and Colon 205 cell lines, where compound 6 had IC(50) values of 0.05, 0.07 and 0.05 mm, respectively. Further, among these effective compounds, 3 and 6 selectively blocked the cell cycle progression at G0/G1, while 1, 2, 4, 7 and 8 blocked the same at G2/M phase of the Jurkat cell line. This is the first cell cycle analysis for the above mentioned isolates on the Jurkat cells. Therefore, these plant-derived compounds may play a role in the prevention and/or management of cancer.

In vivo antitumor potential of extracts from different parts of Bauhinia variegata linn. Against b16f10 melanoma tumour model in c57bl/6 mice

“…For therapeutic effectiveness, drugs are being developed that act as biological modifiers, regulating the cell cycle and promoting cell death [59]. Plant derived compounds have been reported to induce cell cycle arrest and cell death in many tumor cell lines [60][61][62][63][64][65].…”

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“…The cytotoxic activities of these compounds have been evaluated against various cell lines and found that these isolates have a potent tumour inhibitory activity against all investigated cell lines 35 .…”

In vitro evaluation of anti-proliferative properties of the methanolic extracts of Andrographis echioides Nees on skin melanoma cell lines

“…However toxicity related side effects are more common and are highly expensive and thus are far beyond reach of people who live in underdeveloped and poor countries [12][13][14]. Recent studies demonstrated that Andrographis plants particularly, A. paniculata possess anticancerous properties against several cancers [15][16][17]. However, recent studies revealed that there are side effects associated with A. paniculata that includes gastric upset, headache, bitter taste and fatigue and high concentrations of A. paniculata may disturb normal liver function.…”