Cytotoxic effect induced apoptosis in lung cancer cell line on Ageratina adenophora leaf extract

Suganya, Mani; Karthik, Namachivayam; Shivaji, Kavitha; Balasubramanian, M; Ponmurugan, P.

doi:10.1016/j.bcab.2019.101381

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…Sesquiterpenoids, triterpenes, flavonoids, phenolics, coumarins, steroids and phenylpropanols have been isolated and identified from E. adenophorum (Liu et al 2021 ). Various pharmacological studies revealed that E. adenophorum extract had antimicrobial (Manandhar et al 2019 ), antioxidant (Lallianrawna et al 2013 ), anticancer (Mani et al 2019 ), wound healing (Garg and Paliwal 2011 ), analgesic (Mandal et al 2005 ), antipyretic (Ringmichon and Gopalkrishnan 2017 ), anti-inflammatory (Shi et al 2019 ) activities and antiviral activity against COVID-19 main protease (Neupane et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles using Eupatorium adenophorum leaf extract: characterizations, antioxidant, antibacterial and photocatalytic activities

et al. 2023

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The green synthesis of metallic nanoparticles has tremendous impacts in various fields as found in recent years due to their low cost, easy and environmentally friendly synthesis. In this article, we report a simple and eco-friendly method for the synthesis of silver nanoparticles (AgNPs) using an aqueous Eupatorium adenophorum ( E. adenophorum ) leaf extract as a bioreductant. Interestingly, Fourier transform infrared (FTIR) spectroscopy analysis established that the E. adenophorum extract not only served as a bioreductant but also acted as a capping agent to stabilize the nanoparticles by functionalizing the surfaces. Various characterization techniques were adopted, such as X-ray powder diffraction (XRD), FTIR, ultraviolet–visible absorption (UV–Vis) spectroscopy, dynamic light scattering, scanning electron microscopy and energy-dispersive X-ray spectroscopy (EDX) to analyze the biosynthesized AgNPs. Biosynthesized nanoparticles were also explored for antioxidant, antibacterial and photocatalytic activities. The AgNPs showed improved free radical scavenging activity (IC 50 48.96 ± 0.84 µg/mL) and bacterial inhibitory effects against both gram-positive ( Staphylococcus aureus ; 64.5 µg/mL) and gram-negative ( Escherichia coli ; 82.5 µg/mL) bacteria. Photocatalytic investigation showed AgNPs were effective at degrading rhodamine dye (78.69% in 90 min) when exposed to sunlight. These findings collectively suggest that E. adenophorum AgNPs were successfully prepared without the involvement of any hazardous chemical and it may be an effective antibacterial, antioxidant and promising agent for the removal of hazardous dye from waste water produced by industrial dyeing processes.

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Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles using Eupatorium adenophorum leaf extract: characterizations, antioxidant, antibacterial and photocatalytic activities

et al. 2023

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“…The MTT assay is a commonly used method to test biocompatibility 40 – 42 . Previous reports have shown that EAS extracts were cytotoxic, such as the EASL ethanol extract to Hale cells 12 , the EASL methanol extracts to lung cancer cells (A549) 17 , and the EASL essential oil to HePG2, Hep3B, SMMC-7721, and LO2 cells 11 . However, there have been no studies on the cytotoxicity of EASL-AE on Caco-2 cells.…”

Section: Discussionmentioning

confidence: 97%

“…Despite this, EAS continues to be utilized in traditional medicine across various regions, with reported applications in treating wounds, diabetes, inflammation, fever, jaundice, and dysentery 11 – 14 . Modern pharmacological studies have highlighted the diverse biological activities of EAS extracts, including antioxidant 15 , antibacterial 16 , anticancer 17 , wound healing 18 , analgesic 19 , antipyretic 20 , and anti-inflammatory activities 21 . However, regarding the mechanism of its antioxidant action, existing studies have focused on the assessment of free radical scavenging capacity in vitro and lack in vivo validation.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and anti-inflammatory function of Eupatorium adenophora Spreng leaves (EASL) on human intestinal Caco-2 cells treated with tert-butyl hydroperoxide

Zheng-qiang,

Jun,

Xin-yu

et al. 2024

Sci Rep

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Chronic non-communicable diseases (CNCDs) pose a significant public health challenge. Addressing this issue, there has been a notable breakthrough in the prevention and mitigation of NCDs through the use of antioxidants and anti-inflammatory agents. In this study, we aim to explore the effectiveness of Eupatorium adenophora Spreng leaves (EASL) as an antioxidant and anti-inflammatory agent, and its potential applications. To construct a cellular model of oxidative damage and inflammation, Caco-2 cells were treated with tert-butyl hydroperoxide (t-BHP). The biocompatibility of EASL-AE with Caco-2 cells was assessed using the MTT assay, while compatibility was further verified by measuring LDH release and the protective effect against oxidative damage was also assessed using the MTT assay. Additionally, we measured intracellular oxidative stress indicators such as ROS and 8-OHdG, as well as inflammatory pathway signalling protein NFκB and inflammatory factors TNF-α and IL-1β using ELISA, to evaluate the antioxidant and anti-inflammatory capacity of EASL-AE. The scavenging capacity of EASL-AE against free radicals was determined through the DPPH Assay and ABTS Assay. Furthermore, we measured the total phenolic, total flavonoid, and total polysaccharide contents using common chemical methods. The chemical composition of EASL-AE was analyzed using the LC–MS/MS technique. Our findings demonstrate that EASL-AE is biocompatible with Caco-2 cells and non-toxic at experimental levels. Moreover, EASL-AE exhibits a significant protective effect on Caco-2 cells subjected to oxidative damage. The antioxidant effect of EASL-AE involves the scavenging of intracellular ROS, while its anti-inflammatory effect is achieved by down-regulation of the NFκB pathway. Which in turn reduces the release of inflammatory factors TNF-α and IL-1β. Through LC–MS/MS analysis, we identified 222 compounds in EASL-AE, among which gentianic acid, procaine and L-tyrosine were the compounds with high antioxidant capacity and may be the effective constituent for EASL-AE with antioxidant activity. These results suggest that EASL-AE is a natural and high-quality antioxidant and anti-inflammatory biomaterial that warrants further investigation. It holds great potential for applications in healthcare and other related fields.

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Production of bioactive compounds from callus of Pueraria thomsonii Benth with promising cytotoxic and antibacterial activities

Liu

Kumar

Qiu

et al. 2022

Arabian Journal of Chemistry

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Cytotoxic effect induced apoptosis in lung cancer cell line on Ageratina adenophora leaf extract

Cited by 6 publications

References 13 publications

Green synthesis of silver nanoparticles using Eupatorium adenophorum leaf extract: characterizations, antioxidant, antibacterial and photocatalytic activities

Green synthesis of silver nanoparticles using Eupatorium adenophorum leaf extract: characterizations, antioxidant, antibacterial and photocatalytic activities

Antioxidant and anti-inflammatory function of Eupatorium adenophora Spreng leaves (EASL) on human intestinal Caco-2 cells treated with tert-butyl hydroperoxide

Production of bioactive compounds from callus of Pueraria thomsonii Benth with promising cytotoxic and antibacterial activities

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