Cytotoxic Effect of Natural trans-Resveratrol Obtained from Elicited Vitis vinifera Cell Cultures on Three Cancer Cell Lines

Abstract

trans-Resveratrol (trans-R) has been reported to be a potential cancer chemopreventive agent. Although its cytotoxic activity against different cancer cell lines has been tested, its effect on human acute leukemia cell lines has scarcely been investigated, and only a few in vitro studies were performed using human breast epithelial cell lines. Due to its potential value for human health, demand for trans-R has rapidly increased, and new biotechnological strategies to obtain it from natural edible sources have been developed. Thus, grapevine cell cultures represent a reliable system of trans-R production since they biosynthesize trans-R constitutively or in response to elicitation. In addition, there are no studies deepen on the inhibitory effect of trans-R, produced by elicited grapevine cell cultures, on growth of human tumor cell lines. In this work, the effect of trans-R extracted from the culture medium, after elicitation of grapevine cell cultures, was tested on two human acute lymphocytic and monocytic leukemia cell lines, and one human breast cancer cell line. The effect of trans-R on cell proliferation was not only dose- and time-dependent but also cell type-dependent, as seen from the different degrees of susceptibility of cancer cell lines tested. As regards the effect of trans-R on cell cycle distribution, low trans-R concentrations increased cells in the S phase whereas a high trans-R concentration increased G₀/G₁ phase in all cell lines. Perturbation of the cell cycle at low trans-R concentrations did not correlate with the induction of cell death, whereas a high trans-R concentration, cell proliferation decreased as a result of increasing apoptosis in the three cell lines. In leukemia cells, trans-R up-regulated the expression of caspase-3 while trans-R-induced apoptosis in breast cells occur through a caspase-3-independent mechanism mediated by a down-regulation of Bcl-2.

Unraveling the Anticancer Effect of Curcumin and Resveratrol

“…In all four-cell lines, this polyphenol was able to induce apoptosis by reducing Bcl-2 levels and increase caspase-3 activity. Furthermore, in pancreatic cells was also demonstrated an up-regulation in Bax and downregulation in Bcx-xL and XIAP, and in liver cancer cells an increase in p53 expression protein was also detected [299,300,301]. …”

Structure Identification of Triterpene from the Mushroom Pleurotus eryngii with Inhibitory Effects Against Breast Cancer

“…The proliferation inhibition activity of the three isolated compounds against the human breast cancer cell line MCF-7 was evaluated using the MTT assay [22]. As a colourimetric assay, the MTT assay uses cellular metabolic activity to convert the colourless tetrazolium to the purple-coloured formazan salt, which can be quantified by measuring the absorbance at 570 nm.…”

Pomegranate Seed Oil Exerts Synergistic Effects with <i>trans</i>-Resveratrol in a Self-nanoemulsifying Drug Delivery System

“…An in vitro anticancer study showed that the inhibitory rate of RES SNEDDS-PSO against MCF-7 breast cancer cells was 2.03-and 1.24-fold that of RES SNEDDS-IP at a concentration of 12.5 and 25 µg/mL, respectively. This study demonstrated that the newly developed SNEDDS may be a prospective formulation in the functional food and clinical fields.Key words pomegranate seed oil; self-nanoemulsification; trans-resveratrol; anti-inflammation; anticancer activity trans-Resveratrol (RES), a natural component, possesses broad therapeutic activities in the treatment of cancer, 1,2) inflammation, 3,4) neurodegenerative disorders, 5) hyperlipemia, 6) and so on. 7) However, trans-resveratrol application in clinic is scare due to its poor water-solubility, short half-life of 109 min, 8) and undesirable oral bioavailability.…”

“…Key words pomegranate seed oil; self-nanoemulsification; trans-resveratrol; anti-inflammation; anticancer activity trans-Resveratrol (RES), a natural component, possesses broad therapeutic activities in the treatment of cancer, 1,2) inflammation, 3,4) neurodegenerative disorders, 5) hyperlipemia, 6) and so on. 7) However, trans-resveratrol application in clinic is scare due to its poor water-solubility, short half-life of 109 min, 8) and undesirable oral bioavailability.…”