Cytotoxic genes from traditional Chinese medicine inhibit tumor growth both in vitro and in vivo

Zhang, Yuan Hui; Wang, Yuan; Yusufali, Ali Hussein; Ashby, Frederick; Zhang, Dongke; Yin, Zi; Aslanidi, George; Srivastava, Arun; Ling, Chang Quan; Chen, Ling

doi:10.1016/s2095-4964(14)60057-1

Cited by 77 publications

(29 citation statements)

References 39 publications

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“…Total RNA was extracted with Trizol (Life Technologies, China) and reverse-transcribed with a first-strand cDNA synthesis kit (Roche Diagnostics). TaqMan PCR was performed as described (24,25). Stem cell marker mRNA levels were standardized by GAPDH levels and expressed as relative ratio to those of sphere cells treated with control siRNA.…”

Section: Real-time Pcr and Western Blot Analysismentioning

confidence: 99%

CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma

Chen¹,

Liang²,

Wang³

et al. 2017

Preprint

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Renal cell carcinoma (RCC) is the most common kidney cancer, accounting for approximately 80-90 % of all primary kidney cancer. Treatment for patients with advanced RCC remains unsatisfactory. Rare cancer stem cells (CSCs) are proposed to be responsible for failure of current treatment. Here, we investigate the effects of CBFA2T2 on CSCs regulation in RCC, as well as to elucidate the possible mechanisms. We showed that CBFA2T2 expression can significantly predict the survival of RCC patients. Functional assays showed that knocking-down of CBFA2T2 can inhibit cell migration and invasion in 786-O and A-498 cells in vitro, and reduce ALDH high CSCs populations. Furthermore, CBFA2T2 expression is necessary for sphere-forming ability and cancer stem cells marker expression in RCC cell lines. Collectively, our data suggest that CBFA2T2 expression correlates with aggressive characteristics of RCC and CBFA2T2 is required for maintenance of "stemness" through regulation of stem cells factors, thereby highlighting CBFA2T2 as a potential therapeutic target for RCC treatment.KEYWORDS: CBFA2T2; renal cell carcinoma; cancer stem cells; OCT-4; NANOG INTRODUCTIONRenal cell carcinoma (RCC) represents one of the most common type of renal cancer, accounting for 90% of adult renal malignancies (1). The prognosis of advanced RCC is very poor, with a 5-year survival rate of 5-10% (2). In addition, around 20-40% of RCC patients developed recurrence after treatment (3). The mechanisms of how these metastasis and recurrence occur are largely uncharacterized. Recent studies have shown the presence of cancer stem cells (CSCs) in various solid tumor tissues (4-6). CSCs are undifferentiated multipotential tumor cells that have high tumorigenic activity and the capacity to self-renew (4, 5, 7). Moreover, CSCs promote cancer resistance to treatment and recurrence, leading to high mortality (5). Therefore, CSC-targeted therapy can be an essential part of cancer therapy.Previously, CSCs in RCC have been identified by using either stem cell markers (CD133, CD44, CXCR4 and CD105) (8-11) or functional assays (sphere-forming ability, side population and ALDH activity) (12)(13)(14). Especially, in RCC cell lines, ALDH high cells showed CSC properties in vitro, such as clonogenic and self-renewal ability and increased expression of OCT4 and NANOG (15). Importantly, in the metastatic RCC cell lines, ALDH high cells formed about 15% of the total number of cells and had higher Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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Section: Real-time Pcr and Western Blot Analysismentioning

confidence: 99%

CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma

Chen¹,

Liang²,

Wang³

et al. 2017

Preprint

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“…In a murine xenograft model, the gene of the type 1 RIP trichosanthin was delivered by a viral vector to human hepatocellular carcinoma tumour via intra-tumour injection, resulting in a significant inhibition of tumour growth [170].…”

Section: Modern Medicinal Use Of Plant Producing Ribosome-inactivatinmentioning

confidence: 99%

Plants Producing Ribosome-Inactivating Proteins in Traditional Medicine

et al. 2016

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Ribosome-inactivating proteins (RIPs) are enzymes that deadenylate nucleic acids and are broadly distributed in the plant kingdom. Many plants that contain RIPs are listed in the pharmacopoeias of folk medicine all over the world, mostly because of their toxicity. This review analyses the position occupied in traditional medicine by plants from which RIPs have been isolated. The overview starts from the antique age of the Mediterranean area with ancient Egypt, followed by the Greek and Roman classic period. Then, the ancient oriental civilizations of China and India are evaluated. More recently, Unani medicine and European folk medicine are examined. Finally, the African and American folk medicines are taken into consideration. In conclusion, a list of RIP-expressing plants, which have been used in folk medicine, is provided with the geographical distribution and the prescriptions that are recommended by traditional healers. Some final considerations are provided on the present utilization of such herbal treatments, both in developing and developed countries, often in the absence of scientific validation. The most promising prospect for the medicinal use of RIP-expressing plants is the conjugation of purified RIPs to antibodies that recognise tumour antigens for cancer therapy.

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“…Human embryonic kidney cell line, HEK293, and human hepatocellular carcinoma cell line, Huh7, were described previously, 18,19 and maintained as monolayer cultures in DMEM (Invitrogen) supplemented with 10% fetal bovine serum (Sigma) and antibiotics (Lonza).…”

Section: Cells and Reagentsmentioning

confidence: 99%

Cytotoxic genes from traditional Chinese medicine inhibit tumor growth both in vitro and in vivo

Cited by 77 publications

References 39 publications

CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma

CBFA2T2 is Associated with a Cancer Stem Cell State in Renal Cell Carcinoma

Plants Producing Ribosome-Inactivating Proteins in Traditional Medicine

Development of Optimized AAV Serotype Vectors for High-Efficiency Transduction at Further Reduced Doses

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