Cytotoxic Ribonucleases and RNA Interference (RNAi)

Ardelt, Barbara; Ardelt, Wojciech; Darżynkiewicz, Zbigniew

doi:10.4161/cc.2.1.232

Cited by 64 publications

(60 citation statements)

References 32 publications

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“…Several technical reports and hypothesis suggest that siRNA may be the targets of ribonucleases. 34,35 One problem may be an increase in the level of ribonuclease in electropulsed muscles. One way to circumvent this problem could be to use protective compounds and/or to increase the concentrations of siRNAs used for the in vivo transfection experiments.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of gene expression in mice muscle by in vivo electrically mediated siRNA delivery

et al. 2004

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Owing to their capacity to induce strong, sequence-specific, gene silencing in cells, short interfering RNAs (siRNAs) represent new potential therapeutic tools. This development requires, however, new safe and efficient in vivo siRNA delivery methods. In the present technical report, we show that electrically mediated siRNA transfer can suppress transgene expression in adult mice muscles. Using electropulsation for siRNA delivery opens the way for a targeted gene silencing on a broad range of tissues. Clinical applications of electropulsation for delivery of other classes of molecules are under trials. We reported that gene silencing was efficiently obtained in vivo in an adult mammal (mouse) with chemically synthesized siRNA after its electrical delivery. The associated gene silencing was followed on the same animal and lasted at least 11 days. Gene silencing was obtained in muscles not only on young adult mice but also on much older animals. No tissue damages were detected under our electrical conditions. Therefore, this method should provide an efficient approach for a localized delivery of siRNAs in various tissues and organs. Gene Therapy (2005) 12, 246-251.

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Section: Discussionmentioning

confidence: 99%

Inhibition of gene expression in mice muscle by in vivo electrically mediated siRNA delivery

et al. 2004

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“…The latter assumption was put forward for some other antitumor ribonucleases. 13,21,35,36,39,40 eight injections within 2 wk was administered. RLS 40 -bearing mice and mice with metastatic model of B-16 were treated by intraperitoneal administration of binase at doses of 1 and 5 mg/ kg thrice a week within 2 wk, starting on day 4 after tumor transplantation.…”

Section: Discussionmentioning

confidence: 99%

Ribonuclease binase inhibits primary tumor growth and metastases via apoptosis induction in tumor cells

et al. 2013

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“…To reveal whether cell growth on MTPs was suppressed by antitumor drugs and whether the suppression could be monitored by MTP-FR, the T-24 cells were seeded on MTPs and left in cultures either untreated (control) or treated with analog of camptothecin topotecan, the DNA topoisomerase 1 inhibitor (29), or with the cytotoxic ribonuclease onconase (30,31). A decrease in fluorescence intensity of MTPs from the cultures exposed to topotecan and onconase measured by fluorescence microscopy was 27 and 24%, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

Microtransponders, the miniature RFID electronic chips, as platforms for cell growth in cytotoxicity assays

et al. 2006

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Background:An electronic radio frequency (RF) microchip, the microtransponder (MTP), has been developed as a platform for assays in the fields of genomics and proteomics. Upon activation by light, each MTP provides a unique RF identification (ID) signal that matches a chip to the specific biological material attached to it. The MTP is powered by a photocell and has an antenna that transmits the signal. The aim of the present study was to explore utility of MTPs as a platform for cell growth in cytotoxicity assays.Methods:The MCF‐7, MCF‐116, A549, or T‐24 cells growing on MTPs placed in petri dishes or slide chambers were cultured untreated or exposed to antitumor drugs topotecan, mitoxantrone, or onconase for up to 4 days. Their attachment to‐ and growth on‐ MTPs was assessed by fluorescence microscopy and laser scanning cytometry (LSC) and compared with growth on the dish surface in the MTP neighborhood. The MTPs were fixed in ethanol, stained with propidium iodide (PI), and interrogated in flow in the instrument capable to rapidly (up to 103 MTPs/s) identify their ID signal and measure fluorescence.Results:The cells plated on MTPs exhibited similar attachment properties to those plated in culture dishes. When measured by LSC, they had similar mitotic activity, growth rate, and cell cycle distributions as the cells adhering to the culture dish in the neighborhood of MTPs. The fluorescence intensity of MTPs provided information about the cell number per MTP, which made it possible to assess cell growth rate and monitor the cytostatic/cytotoxic effects of the tested drugs.Conclusions:The MTP‐based system holds promise for the multiplexed cell assays in which numerous different cell lines can be screened for their growth rate or sensitivity while exposed to particular agents in the same vessel. Other advantages of the system are the rapidity of the screening and a very large number of ID codes. Because many cell lines/types can be assayed in a single dish, the system also offers cost savings on tissue culture reagents. © 2006 International Society for Analytical Cytology

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Cytotoxic Ribonucleases and RNA Interference (RNAi)

Cited by 64 publications

References 32 publications

Inhibition of gene expression in mice muscle by in vivo electrically mediated siRNA delivery

Inhibition of gene expression in mice muscle by in vivo electrically mediated siRNA delivery

Ribonuclease binase inhibits primary tumor growth and metastases via apoptosis induction in tumor cells

Microtransponders, the miniature RFID electronic chips, as platforms for cell growth in cytotoxicity assays

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