Cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities of <i>Hygrophila schulli</i>

Abstract

The crude methanol extract of Hygrophila schulli and its petroleum ether, chloroform, ethyl acetate and aqueous soluble Kupchan partitionates were investigated for in vitro cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities. In the brine shrimp lethality bioassay, the petroleum ether soluble extract of H. schulli showed significant cytotoxicity (LC50 = 0.1 µg/mL and LC90 = 15 µg/mL) as compared to vincristine sulfate (LC50 = 0.4 µg/mL and LC90 = 9 µg/mL). Among all the partitionates, chloroform soluble fraction demonstrated the highest thrombolytic activity with 10.5% clot lyses. Moreover, in hypotonic-and heat-induced conditions, the chloroform soluble extractive inhibited hemolysis of human erythrocyte by 113.7% and 14.3%, respectively as compared to 71.9% and 42.2% demonstrated by standard acetylsalicylic acid. On the other hand, in anti-oxidant activity test, chloroform soluble fraction revealed mild antioxidant activity (IC50 = 195.1 µg/mL) as compared to standard tert-butyl-1-hydroxytoluene (IC50 = 27.5 µg/ mL).

Screening Phytochimique, Étude Toxicologique, Évaluation des Activités Antiplasmodiale et Antiradicalaire de la Tige Feuillée de Senna occidentalis Linn (Fabaceae)

“…En effet, lors d'une évaluation toxicologique d'extraits de plantes par ce test de toxicité, une valeur de CL50 < 1000 g/mL est considérée comme bioactive (Meyer et al, 1982). Il est un dépistage préliminaire de la toxicité des extraits végétaux (Ghosh et al, 2015;Kibiti et Afolayan, 2016;Oberlies et al, 1998;Sufian et Haque, 2015;Syahmi et al, 2010), toxines fongiques (Harwing et Scott., 1971), métaux lourds (Saliba et Krzyz, 1976), toxines cyanobactériennes (Hisem et al, 2011), pesticides (Michael et al, 1956), tests de cytotoxicité sur des matériaux dentaires (Pelka et al, 2000) et nanostructures (Maurer-Jones et al, 2013). Pour le composé bioactif d'origine naturelle ou synthétique, il s'agit d'un test rapide et complet (Quazi et al, 2017).…”

Brine shrimp lethality assay

“…It is a preliminary toxicity screening of plant extracts (Ghosh et al, 2015;Kibiti and Afolayan, 2016;Oberlies et al, 1998;Sufian and Haque, 2015;Syahmi et al, 2010), fungal toxins (Harwing and Scott, 1971), heavy metals (Saliba and Krzyz, 1976), cyanobacteria toxins (Hisem et al, 2011), pesticides (Michael et al, 1956), cytotoxicity testing of dental material (Pelka et al, 2000) and nanostructures (Maurer-Jones et al, 2013). Subsequently animal model is recommended for its establishment.…”

Pharmacognostic, phytochemical, biological and spectroscopic analyses of Capparis decidua (Forsk.) Edgew root and stem bark

“…Brine shrimp (Artemia salina) lethality assay is a preliminary toxicity screening test commonly used to check the cytotoxic effect of bioactive chemicals [22]. In the present research work, methanolic root bark extract showed significant activity at higher concentration, while methanolic stem bark shows no activity.…”