Cytotoxicity Evaluation and Subcellular Location of Titanium Dioxide Nanotubes

Wang, Yanli; Sui, Keke; Fang, Jie; Yao, Chenjie; Yuan, Lulu; Wu, Qiuxia; Wu, Minghong

doi:10.1007/s12010-013-0447-0

Cited by 6 publications

(5 citation statements)

References 31 publications

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“…The viability of GES-1 cells in each well was measured using a CCK-8 kit according to the manufacturer’s instructions. Cell viability is expressed as the percentage of viable cells in the total number of cells …”

Section: Methodsmentioning

confidence: 99%

“…Cell viability is expressed as the percentage of viable cells in the total number of cells. 33 Cell Uptake Study. After three passages, GES-1 cells under good growth conditions were seeded into a culture dish and cultured at 37 °C in a 5% CO 2 atmosphere for 24 h. Then, the culture medium was removed and GES-1 cells were washed with D-Hanks buffer several times.…”

Section: ■ Introductionmentioning

confidence: 99%

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Acute and Subacute Toxicity Study of Graphene-Based Tumor Cell Nucleus-Targeting Fluorescent Nanoprobes

et al. 2020

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Graphene-based tumor cell nuclear targeting fluorescent nanoprobes (GTTNs) were synthesized in our laboratory as a kind of nanomaterial and showed good performance for both in vivo and in vitro imaging. GTTNs directly cross the cell membrane and specifically target the tumor cell nucleus via a cell membrane permeability targeting (CMPT) mechanism, which takes advantage of the increased permeability of the tumor cell membranes. GTTNs with a CMPT mechanism achieve high targeting efficiency in tumor tissues. With the tumor cell nucleus-targeting characterization, the GTTN distinguishes tumor cells at the single-cell level and recognizes the tumor tissue interface in a very early stage and shows great potential in clinical applications. Toxicity studies are extremely critical for clinical applications. Therefore, we studied the acute and subacute toxicity of GTTNs using an in vivo method and examined the following experimental indicators: mouse body weight, organ coefficients, serum biochemical parameters, and histological changes. The results showed that there were no significant differences in any indicators between the experimental and control mice. We also used an in vitro method to study the cytotoxicity of GTTNs in GES-1 (gastric epithelial cell) cells. Surprisingly, the results demonstrated over 80% cell viability when the incubation time reached up to 72 h under a 200 mg/L concentration of GTTNs, which indicated that GTTNs had low cytotoxicity. GTTNs barely showed any acute or subacute toxicity or cytotoxicity in vivo and in vitro, respectively, which supports their use for clinical applications.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Acute and Subacute Toxicity Study of Graphene-Based Tumor Cell Nucleus-Targeting Fluorescent Nanoprobes

et al. 2020

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“…The rate of cellular uptake can be boosted through post-fabrications and surface modifications. A common size-dependent intracellular mechanism and localization roughly show an intracellular trafficking pathway, mostly ending up in endosomes and lysosomes [117,118]. The incubation of TiO 2 nanomaterials can induce both cytotoxic and genotoxic effects on mammalian cells by disrupting mitochondrial membranes [119,120].…”

Section: In Vitro Cytotoxicity Assessmentsmentioning

confidence: 99%

Section: Biological Responses To Tio 2 Nssmentioning

confidence: 99%

Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

Kafshgari

Goldmann

2020

Nano-Micro Lett.

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HIGHLIGHTS • Multifunctional TiO 2 nanostructures hold promise for advancing a wide range of biomedical applications due to a feasible integration of distinct theranostic features. • Fabrication and post-fabrication strategies implemented to generate multifunctional TiO 2 nanostructures for a broad range of biomedical applications are briefly outlined. The opportunities and challenges of TiO 2 nanomaterials are highlighted in order to open the possibility of clinical translation. ABSTRACT Titanium dioxide (TiO 2 ) nanostructures exhibit a broad range of theranostic properties that make them attractive for biomedical applications. TiO 2 nanostructures promise to improve current theranostic strategies by leveraging the enhanced quantum confinement, thermal conversion, specific surface area, and surface activity. This review highlights certain important aspects of fabrication strategies, which are employed to generate multifunctional TiO 2 nanostructures, while outlining post-fabrication techniques with anemphasis on their suitability for nanomedicine. The biodistribution, toxicity, biocompatibility, cellular adhesion, and endocytosis of these nanostructures, when exposed to biological microenvironments, are examined in regard to their geometry, size, and surface chemistry. The final section focuses on recent biomedical applications of TiO 2 nanostructures, specifically evaluating therapeutic delivery, photodynamic and sonodynamic therapy, bioimaging, biosensing, tissue regeneration, as well as chronic wound healing.

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“…Interactions between the cells and NPs, including cellular uptake, subcellular localization, and metabolism present the most primitive fundamental phenomenon, which are very important for the study of NPs toxicity. Wang et al [37] studied the sub-cellular location of TiO2 nanotubes (TiO2-NTs) in neural stem cells. It is reported that TiO2-NTs can be internalized into the cells after 24 h of incubation.…”

Section: Titanium Dioxide Nanoparticlesmentioning

confidence: 99%

Toxic effects of metal oxide nanoparticles and their underlying mechanisms

et al. 2017

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Nanomaterials have attracted considerable interest owing to their unique physicochemical properties. The wide application of nanomaterials has raised many concerns about their potential risks to human health and the environment. Metal oxide nanoparticles (MONPs), one of the main members of nanomaterials, have been applied in various fields, such as food, medicine, cosmetics, and sensors. This review highlights the bio-toxic effects of widely applied MONPs and their underlying mechanisms. Two main underlying toxicity mechanisms, reactive oxygen species (ROS)-and non-ROS-mediated toxicities, of MONPs have been widely accepted. ROS activates oxidative stress, which leads to lipid peroxidation and cell membrane damage. In addition, ROS can trigger the apoptotic pathway by activating caspase-9 and-3. Non-ROS-mediated toxicity mechanism includes the effect of released ions, excessive accumulation of NPs on the cell surface, and combination of NPs with specific death receptors. Furthermore, the combined toxicity evaluation of some MONPs is also discussed. Toxicity may dramatically change when nanomaterials are used in a combined system because the characteristics of NPs that play a key role in their toxicity such as size, surface properties, and chemical nature in the complex system are different from the pristine NPs.

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Cytotoxicity Evaluation and Subcellular Location of Titanium Dioxide Nanotubes

Cited by 6 publications

References 31 publications

Acute and Subacute Toxicity Study of Graphene-Based Tumor Cell Nucleus-Targeting Fluorescent Nanoprobes

Acute and Subacute Toxicity Study of Graphene-Based Tumor Cell Nucleus-Targeting Fluorescent Nanoprobes

Insights into Theranostic Properties of Titanium Dioxide for Nanomedicine

Toxic effects of metal oxide nanoparticles and their underlying mechanisms

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